Recombinant Chicken Sclerostin domain-containing protein 1 (SOSTDC1)

Code CSB-YP764958CH
MSDS
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Source Yeast
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Code CSB-EP764958CH
MSDS
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Source E.coli
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Code CSB-EP764958CH-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP764958CH
MSDS
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Source Baculovirus
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Code CSB-MP764958CH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SOSTDC1
Uniprot No.
Alternative Names
SOSTDC1; WISE; Sclerostin domain-containing protein 1; Wnt-signaling modulator
Species
Gallus gallus (Chicken)
Expression Region
23-206
Target Protein Sequence
AFKNDATE ILYSHVVKPA PASPSSNSTL NQARNGGRHY AGTGSDRNNR VQVGCRELRS TKYISDGQCT SINPLKELVC AGECLPLPLL PNWIGGGYGT KYWSRRSSQE WRCVNDKTRT QRIQLQCQDG SIRTYKITVV TACKCKRYTR QHNESSHNFE GTSQAKPVQH HKERKRASKS SKHSTS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Can activate or inhibit Wnt signaling in a context-dependent manner. Activates the canonical Wnt pathway whereby acts through Disheveled proteins and beta-catenin. Antagonises Wnt signaling through the canonical pathways presumably by blocking accessibility of certain WNTs to their receptors. Induces posterior neural markers via components of the canonical Wnt pathway.
Gene References into Functions
  1. Wise is expressed in regions where other signaling molecules such as Wnt, Bmp, and Shh are known to function in morphogenesis and differentiation. PMID: 17654720
  2. While secreted Wise either synergizes or inhibits the Wnt signals depending on the partner ligand, ER-retained Wise consistently blocks the Wnt pathway. ER-retained Wise reduces LRP6 on the cell surface, making cells less susceptible to the Wnt signal. PMID: 17765217
  3. Wise is able to function cell non-autonomously on neural crest cells and promote trigeminal ganglion formation. PMID: 18538759
Subcellular Location
Secreted.
Protein Families
Sclerostin family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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