Recombinant Chicken Semaphorin-3A (SEMA3A), partial

Code CSB-YP841905CH
MSDS
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Source Yeast
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Code CSB-EP841905CH
MSDS
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Source E.coli
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Code CSB-EP841905CH-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP841905CH
MSDS
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Source Baculovirus
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Code CSB-MP841905CH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SEMA3A
Uniprot No.
Alternative Names
SEMA3A; COLL1; Semaphorin-3A; Collapsin-1; COLL-1
Species
Gallus gallus (Chicken)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to neuropilin.
Gene References into Functions
  1. These findings suggest that by interacting with PlexA4, TrkA plays a crucial role in redirecting local Sema3A signaling to retrograde axonal transport, thereby regulating dendritic GluA2 localization and patterning. PMID: 26945060
  2. Results indicate that GSK3beta and Axin-1 are involved in Sema3A-induced bidirectional axonal transport PMID: 25528666
  3. Axon guidance in the developing ocular motor system and Duane retraction syndrome depends on Semaphorin signaling via alpha2-chimaerin. PMID: 22912401
  4. these results identify a novel mechanism of Sema3A-induced axonal transport, and further suggest that Sema3A-induced local calcium signaling in growth cone is propagated to cell body in a TTX-sensitive manner. PMID: 22575508
  5. Syx1B is involved in the regulation of macropinocytosis in semaphorin (Sema)3A-induced growth cone collapse. PMID: 21593320
  6. Recombinant Sema3A protein attenuated mechanical allodynia and heat hyperalgesia in chronic constriction injury (CCI) rats, whereas heat-inactivated Sema3A had no effect. PMID: 20888378
  7. lens-derived Sema3A mediates initial repulsion of trigeminal sensory axons from the cornea and is necessary for the proper formation of the nerve ring and positioning of the ventral plexus in the choroid fissure PMID: 17499699
  8. Sema3A overexpression induced defasciculation and exuberant growth of motor axon projections into these normally non-permissive territories. PMID: 18039974

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Subcellular Location
Secreted.
Protein Families
Semaphorin family
Tissue Specificity
Expressed at relatively high levels in brain and muscle, moderate levels in lung, bursa, and heart and virtually absent in liver. Collapsin-1, -2, -3, and -5 bind to overlapping but distinct axon tracts.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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