Recombinant Corynebacterium glutamicum Glutamate N-acetyltransferase (argJ)

Code CSB-YP704543DWY
MSDS
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Source Yeast
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Code CSB-EP704543DWY
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Source E.coli
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Code CSB-EP704543DWY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP704543DWY
MSDS
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Source Baculovirus
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Code CSB-MP704543DWY
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
argJ
Uniprot No.
Alternative Names
argJ; Cgl1395; cg1581Arginine biosynthesis bifunctional protein ArgJ [Cleaved into: Arginine biosynthesis bifunctional protein ArgJ alpha chain; Arginine biosynthesis bifunctional protein ArgJ beta chain] [Includes: Glutamate N-acetyltransferase; EC 2.3.1.35; Ornithine acetyltransferase; OATase; Ornithine transacetylase); Amino-acid acetyltransferase; EC 2.3.1.1; N-acetylglutamate synthase; AGSase)]
Species
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Expression Region
1-182
Target Protein Sequence
MAEKGITAPK GFVASATTAG IKASGNPDMA LVVNQGPEFS AAAVFTRNRV FAAPVKVSRE NVADGQIRAV LYNAGNANAC NGLQGEKDAR ESVSHLAQNL GLEDSDIGVC STGLIGELLP MDKLNAGIDQ LTAEGALGDN GAAAAKAIMT TDTVDKETVV FADGWTVGGM GKGVGMMAPS LA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate.
Gene References into Functions
  1. To enhance l-ornithine production, the argJ gene from C. glutamicum ATCC 13032 was overexpressed. In flask cultures, the resulting strain, C. glutamicum 1006argR-argJ, produced 31.6 g/L l-ornithine, which is 54.15% more than that produced by C. glutamicum 1006. PMID: 25630515
  2. Its homologous expression provides the N-acetylglutamate synthase activity that supplies additional N-acetylglutamate for L-ornithine/L-arginine biosynthesis in Corynebacterium glutamicum. PMID: 20544254
Subcellular Location
Cytoplasm.
Protein Families
ArgJ family
Database Links

KEGG: cgb:cg1581

STRING: 196627.cg1581

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