Recombinant Desulfobacterium autotrophicum Phosphomethylpyrimidine synthase (thiC)

Code CSB-YP507890DJK
MSDS
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Source Yeast
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Code CSB-EP507890DJK
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Source E.coli
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Code CSB-EP507890DJK-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP507890DJK
MSDS
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Source Baculovirus
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Code CSB-MP507890DJK
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
bzaF
Uniprot No.
Alternative Names
bzaF; thiC; HRM2_06760; 5-hydroxybenzimidazole synthase; 5-OHBza synthase; HBI synthase; EC 4.1.99.23
Species
Desulfobacterium autotrophicum (strain ATCC 43914 / DSM 3382 / HRM2)
Expression Region
1-439
Target Protein Sequence
MKTQIELARD GIISRQMEQV AADENFNPEI IRTRVAAGEI VIPCNPNRTN QKVVGIGTGL RTKINASIGT SSDICSIENE VQKAIAIEEE GADTLMELSA GGNLDKVRQE VLKAVNLPVG NVPLYQAFKE TGRKYNDPSK LDPEFLFDLI ERQLSDGLSF MAIHCGINQY TIERLRKQGF RYGGLASKGG TFMVAWMDAT GKENPLYEQF DRVCTLMKKY DAVLSLGNGI RAGAIHDSHD RAQMAEMIIN CELAELGREQ GCQMMVEGPG HVPLDEIQAN IILEKRMSGN APYYVLGPIP ADTGAGYDHI TSAIGAASST WHGADLICYI TPAEHLALPT EADVREGVRA TKLAVRIGDI AKYPDRRENE RLASMARRDM RWDDLEQHLL FPDIARKTRA SRAPEDGGTC TMCGDFCAMK KGNEIFKDDI KNDKIAPGA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the conversion of aminoimidazole ribotide (AIR) to 5-hydroxybenzimidazole (5-HBI) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction. Is thus involved in the anaerobic biosynthesis of the benzimidazole lower axial ligand of the cobamide produced by D.autotrophicum.
Protein Families
ThiC family, 5-hydroxybenzimidazole synthase subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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