Recombinant Epstein-Barr virus Epstein-Barr nuclear antigen 6(EBNA6) ,partial

Code CSB-YP365875EFA
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Source Yeast
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Code CSB-EP365875EFA
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Source E.coli
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Code CSB-EP365875EFA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP365875EFA
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Source Baculovirus
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Code CSB-MP365875EFA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names EBNA6
Uniprot No. P03204
Alternative Names EBNA6; BERF3-BERF4Epstein-Barr nuclear antigen 6; EBNA-6; EBV nuclear antigen 6; Epstein-Barr nuclear antigen 3C; EBNA-3C; EBV nuclear antigen 3C; Epstein-Barr nuclear antigen 4B; EBNA-4B; EBV nuclear antigen 4B
Species Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Plays an essential role for the activation and immortalization of human B-cells. Represses transcription of viral promoters TP1 and Cp through interaction with host RBPJ, and inhibits EBNA2-mediated activation of these promoters. Since Cp is the promoter for all EBNA mRNAs, EBNA6 probably contributes to a negative autoregulatory control loop. Alternatively, EBNA6 also regulates the transcription of the EBV oncogene LMP1 in a cell cycle-dependent manner. Modulates the activity of several host proteins involved in cell cycle regulation including host cyclin A, MYC, RB, p21 and p27 mainly through binding to the host SCF(SKP2) complex. Inhibits the promoter of host H2AX and targets H2AX to proteasomal degradation in order to promote latency and cell proliferation.
Gene References into Functions
  1. Our results provide novel insights into the function of EBNA3C on cell progression by regulating the cyclin D2 protein and raise the possibility of the development of new anticancer therapies against EBV-associated cancers. PMID: 29997218
  2. EBNA3B restrains the oncogenic capacity of EBV, so it acts as a tumour suppressor.[review] PMID: 26428372
  3. EBNA3C is necessary to overcome an EBV-driven increase in p16(INK4a) expression and concomitant block to proliferation 2-4 weeks post-infection. PMID: 23436997
  4. The p73-mediated apoptotic pathway is inhibited in the presence of EBNA3C. PMID: 24314664
  5. During latency, out of a subset of viral genes expressed, EBNA-3C is one of the essential antigens required for in vitro primary B-cell transformation. PMID: 23464371
  6. This study provided formal proof that when EBV represses BIM transcription in B cells, expression of both EBNA3A and EBNA3C is necessary for the assembly of PRC2 on the BIM promoter and the deposition of the epigenetic repressive mark H3K27me3. PMID: 22584624
  7. EBNA3BKO virus caused highly proliferative, monomorphic B cell lymphomas in humanized mice. EBV+ human lymphoma samples had EBNA3B mutations. EBNA3B is a virus-encoded tumor suppressor. Loss promotes immune evasion & lymphomagenesis. PMID: 22406538
  8. we have unveiled key cellular pathways repressed by EBNA 3C that are likely to contribute to the ability of EBV-immortalized cells to modulate immune responses, adhesion, and B-lymphocyte migration to facilitate persistence in the host. PMID: 22357270
  9. EBNA3C gene variations are geographically restricted rather than tumor-specific polymorphisms. PMID: 22302288
  10. we report that EBNA3C directly interacts with Gemin3 through its C-terminal domains PMID: 22174681
  11. Epstein-Barr virus nuclear antigen 3C facilitates G1-S transition by stabilizing and enhancing the function of cyclin D1. PMID: 21347341
  12. EBNA3C negatively regulate p53-mediated functions by interacting with ING4 and ING5. PMID: 21177815
  13. EBV nuclear antigen 3C (EBNA3C) is an essential transcription factor for EBV transformed lymphoblast cell line growth, regulating 550 genes, 106 of which are recognized in protein networks. PMID: 21173222
  14. EBNA3C interacts with Gadd34, activating the upstream component of the unfolded protein response UPR (eIF2alpha phosphorylation) while preventing downstream UPR events (XBP1 activation and ATF6 cleavage). PMID: 20040105
  15. EBNA3C forms a stable complex with Rb in cells when the proteasome machinery is inhibited and interacts with Rb in vitro, mapping to a conserved domain at the terminus of EBNA3C PMID: 16352731
  16. findings show that both EBNA3C and Rhesus EBNA3C bind to a conserved region of mammalian cyclins, regulate pRb stability, and modulate SCF(Skp2)-dependent ubiquitination PMID: 16364389
  17. current knowledge of functions of EBNA 3C, the roles played by the different domains in these functions, and summary of recent work that provides the first structural and functional analysis of the putative bZIP domain of EBNA 3C [review] PMID: 16611138
  18. The effects of the EBV nuclear antigen 3C on the activities of Nm23-H1 via GATA-1 and Sp1 are reported. PMID: 16631833
  19. results indicate that EBNA-3C directly activates the expression of LMP-1 and LMP-2B but is unlikely to significantly regulate EBNA expression via Cp under normal growth conditions.[EBNA-3C] PMID: 16956945
  20. identification of a number of potential EBNA-3B- and EBNA-3C-regulated genes in the context of EBV-infected lymphoblastoid cell lines PMID: 17005691
  21. Epstein-Barr virus nuclear protein EBNA3C is required for cell cycle progression and growth maintenance of lymphoblastoid cells. PMID: 17159137
  22. EBNA3C can directly regulate the G2/M component of the host cell cycle machinery through ATM/ATR and Chk2, allowing for the release of the checkpoint block PMID: 17409144
  23. In the absence of Bim, EBNA3A and EBNA3C appear to provide no survival advantage in Burkitt's lymphoma cells. PMID: 17653091
  24. EBNA-6/EBNA-3C binds to a MRPS18-2 protein, and targets it to the nucleus; EBNA-6 may play a major role in the entry of EBV infected B cells into the S phase by binding to and raising the level of nuclear MRPS18-2, protein. PMID: 18391203
  25. EBNA3C can modulate Necdin-associated functions in association with the metastasis suppressor protein Nm23-H1. PMID: 19116252
  26. EBNA3C regulation of transcription through RBP-Jkappa is critical to maintaining lymphoblastoid cell growth PMID: 19237563
  27. Epstein-Barr virus nuclear antigen 3C, one of the EBV latent antigens essential for the B-cell immortalization in vitro, interacts directly with p53 PMID: 19394062
  28. The authors confirmed that EBNA3C upregulates TCL1 and discovered that EBNA3C upregulates TCL1 through RBP-Jkappa, indicating a central role for EBNA3C interaction with RBP-Jkappa in mediating cell gene transcription. PMID: 19776126

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Subcellular Location Host nucleus. Host nucleus matrix. Note=Associated with the nuclear matrix.
Protein Families Herpesviridae EBNA-6 family
Database Links

KEGG: vg:3783763

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