Recombinant Epstein-Barr virus Replication and transcription activator (BRLF1), partial

Code CSB-YP355981EFA
MSDS
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Source Yeast
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Code CSB-EP355981EFA
MSDS
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Source E.coli
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Code CSB-EP355981EFA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP355981EFA
MSDS
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Source Baculovirus
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Code CSB-MP355981EFA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BRLF1
Uniprot No.
Alternative Names
BRLF1; Replication and transcription activator; Rta; Immediate-early protein Rta
Species
Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Immediate-early transcription factor that controls the initiation of viral lytic gene expression and lytic reactivation from latency. Triggers lytic replication, and initiates a cellular senescence program in epithelial cells. Upregulates human DCR3/TNFRSF6B by directly binding to its receptor.
Gene References into Functions
  1. Rta-mediated decreased binding of CTCF in the viral genome is concurrent with virus reactivation. Via interfering with CTCF binding, in the host genome Rta can function as a transcriptional repressor for gene silencing. PMID: 28490592
  2. Rta autoregulates BRLF1 transcription via its interaction with USF1 on E1. PMID: 26297580
  3. These results suggest that the Epstein-Barr virus genotypes BZLF1-B and BRLF1-C may be associated with the tumorigenesis of lymphoma. PMID: 24615673
  4. BZLF1 and BRLF1 expression in AGS-EBV cells transfected with a miR-BART20-5p inhibitor was enhanced. PMID: 24899173
  5. These interactions are important to the transcriptional activation of these genes by Rta since introducing TAF4 shRNA substantially reduces the ability of Rta to activate these promoters PMID: 23326574
  6. The authors demonstrate that lytic viral gene expression and replication can be induced by BRLF1, but not BZLF1, expression in an epithelial cell line (NOKs-Akata) in which lytic viral gene promoters remain largely unmethylated. PMID: 23135711
  7. expression of the BARF1 protein during lytic replication is regulated by direct binding of BRLF1 to multiple R-responsive elements in the gene control region and is independent of the promoter methylation status PMID: 22896599
  8. findings suggest that Rta plays an active role in redirecting HeLa cell physiology through an Rta-mediated cell cycle transformation PMID: 22149496
  9. Epstein-Barr virus Rta-mediated transactivation of p21 and 14-3-3sigma arrests cells at the G1/S transition by reducing cyclin E/CDK2 activity. PMID: 21918011
  10. The authors demonstrate that the cellular transcription factor Oct-1 cooperates with the EBV immediate-early protein BRLF1 (R, Rta) to induce lytic viral reactivation. PMID: 21697476
  11. mapped the LF2 binding site to Rta amino acids (aa) 476 to 519 and showed that LF2 binding is critical for Rta relocalization and repression PMID: 20631124
  12. Data demonstrated the important roles of Zta and Rta in EBV lytic reactivation. PMID: 20338640
  13. The authors show that BRLF1 expression decreased induction of IFN-beta, and reduced expression of IRF3 and IRF7. PMID: 20381110
  14. A critical role for Rta in regulating the transcription of the genes that are mediated by Sp1. PMID: 16314315
  15. findings show that Epstein-Barr virus Rta interacts and colocalizes with PIASxalpha and PIASxbeta in the nucleus; these interactions seem to enhance Rta sumoylation PMID: 16460827
  16. Epstein-Barr virus transcription activator Rta upregulates decoy receptor 3 expression by binding to its promoter. PMID: 17301127
  17. Results describe the enhancement of the transactivation activity of Epstein-Barr virus Rta protein by RanBPM. PMID: 18455188
  18. study found that Rta, is a binding partner of BRAP2; Rta appeared to prevent the binding of KSR1 to BRAP2, activating the ERK signal transduction pathway and the transcription of an EBV immediate-early gene, BZLF1 PMID: 18796711
  19. The present study demonstrates that Epstein-Barr Virus Rta efficiently elicits growth arrest in the human epithelial cell line HEK293. PMID: 19098430
  20. C-terminal 10 amino acids of Rta play an essential role in DNA binding inhibitory activity. Point mutations of two phenylalanines were essential for inhibiting the DNA binding function. PMID: 19232420

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Protein Families
Herpesviridae Rta family
Database Links

KEGG: vg:3783727

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