Recombinant Epstein-Barr virus Trans-activator protein BZLF1 (BZLF1)

In Stock
Code CSB-EP355980EFA
Size US$2466Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP355980EFA could indicate that this peptide derived from E.coli-expressed Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4) BZLF1.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP355980EFA could indicate that this peptide derived from E.coli-expressed Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4) BZLF1.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names BZLF1
Uniprot No. P03206
Research Area Others
Alternative Names BZLF1; Trans-activator protein BZLF1; EB1; Zebra
Species Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Source E.coli
Expression Region 1-245aa
Target Protein Sequence MMDPNSTSEDVKFTPDPYQVPFVQAFDQATRVYQDLGGPSQAPLPCVLWPVLPEPLPQGQLTAYHVSTAPTGSWFSAPQPAPENAYQAYAAPQLFPVSDITQNQQTNQAGGEAPQPGDNSTVQTAAAVVFACPGANQGQQLADIGVPQPAPVAAPARRTRKPQQPESLEECDSELEIKRYKNRVASRKCRAKFKQLLQHYREVAAAKSSENDRLRLLLKQMCPSLDVDSIIPRTPDVLHEDLLNF
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 42.9kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
(From Uniprot)
Plays a key role in the switch from latent infection to lytic cycle producing new virions. Acts as a transcription factor, inducing early lytic cycle genes, and as a origin binding protein for genome replication. BZLF1 activates the promoter of another EBV gene (BSLF2+BMLF1).
Gene References into Functions
  1. position C189 in Zta impacts sequence-specific binding to DNA containing modified and unmodified cytosine. PMID: 29772230
  2. ERK/c-Jun recruits Tet1 to Induce Zta expression and Epstein-Barr virus reactivation through DNA demethylation. PMID: 27708396
  3. HIF-1alpha directly bound the promoter of the EBV primary latent-lytic switch BZLF1 gene, Zp, activating transcription via a consensus hypoxia-response element (HRE) located at nt -83 through -76 relative to the transcription initiation site. PMID: 28617871
  4. By CRISPR/Cas9-induced inactivation of TLR9, MyD88, IRAK4 and IRAK1 we confirm that BZLF1 repression is dependent on functional TLR9 and MyD88 signaling, and identify IRAK4 as an essential element for TLR9-induced repression of BZLF1 expression upon BCR cross-linking PMID: 29088270
  5. Comparison of how AP-1 (Jun/Jun dimer) and Epstein-Barr virus Zta recognize methyl groups within their cognate response elements PMID: 28158710
  6. Binding of PARP1 at the BZLF1 lytic switch promoter restricts Epstein Barr Virus reactivation. PMID: 28456021
  7. These results suggest that lytic Epstein-Barr virus infection attenuates the transmission of inflammatory paracrine senescence through BZLF1 downregulation of TNF-alpha secretion and alters the inflammatory microenvironment to allow virus propagation and persistence. PMID: 27334596
  8. The dimerization region of Epstein-Barr virus Zta is not required to mediate host CIITA repression. PMID: 26653871
  9. These results suggest that Epstein-Barr Virus BZLF1 attenuates the proinflammatory responses to facilitate viral replication. PMID: 26537683
  10. Presented is a model of DNA damage responses activation during the EBV lytic cycle in which ZEBRA induces ATM kinase phosphorylation, in a DNA binding dependent manner, to modulate gene expression. PMID: 25950714
  11. RanBPM was found to enhance Zta-dependent transcriptional activity via the inhibition of Zta sumoylation. PMID: 25900136
  12. Studied the effects of BZLF1 on cellular response to DNA damage in nasopharyngeal carcinoma cells. PMID: 26006018
  13. This is the first report to characterize BZLF1 gene polymorphisms in paediatric patients from our geographical region and to suggest the association of these polymorphisms with malignant lymphomas. PMID: 24666405
  14. ZEBRA and BGLF5 each function as viral host shutoff factors. PMID: 24705134
  15. these results indicate a critical role for MCAF1 in AP-1-dependent Rta activation of BZLF1 transcription. PMID: 24598729
  16. Performed the recombinant production of a biologically active Epstein-Barr virus BZLF1 trans-activator, i.e., Z-encoded broadly reactive activator (ZEBRA), that recognized specific DNA motifs. PMID: 24291446
  17. B-cell specific transcription factor, Oct-2, inhibits the function of the viral immediate-early protein, BZLF1, and prevents lytic viral reactivation. PMID: 22346751
  18. the genetic characteristics of BZLF1 gene and its promoter Zp of the epidemic strains in children with primary Epstein-Barr virus (EBV)-associated diseases PMID: 24772891
  19. TAF-Ibeta promotes BZLF1 expression and subsequent lytic infection by affecting chromatin at the BZLF1 promoter PMID: 23691099
  20. Mutation of MEF2 sites severely impaired both spontaneous and induced expression of not only BZLF1, but also BRLF1 in comparison to wild-type or revertant virus cases. PMID: 23843637
  21. The B-cell-specific transcription factor and master regulator Pax5 promotes Epstein-Barr virus latency by negatively regulating the viral immediate early protein BZLF1. PMID: 23678172
  22. Zta drives cell migration and invasion through MMPs PMID: 23409137
  23. BZLF1 is a pioneer factor that reverses epigenetic silencing of viral DNA to allow escape from latency. PMID: 22969425
  24. Genome-wide analyses of Zta binding to the Epstein-Barr virus genome reveals interactions in both early and late lytic cycles and an epigenetic switch leading to an altered binding profile. PMID: 23015699
  25. The mechanism of p53-mediated Epstein-Barr viral Zta protein activation requires the cooperative action of another cellular factor, Sp1. PMID: 22711357
  26. Herpes simplex virus type 1 induces the reactivation of latent EBV by triggering BZLF1 expression. PMID: 22188237
  27. Epigenetic modification of the BZLF1 promoter in latent Raji cells by histone H3 lysine 27 trimethylation (H3K27me3), H3K9me2/me3, and H4K20me3. PMID: 22357272
  28. The authors show that expression of the Epstein-Barr virus immediate-early protein BZLF1 is restricted to Blimp1-positive epithelial cells in oral hairy leukoplakia. PMID: 22278826
  29. The abundance of Zta protein coupled with the methylation status of the Epstein-Barr virus (EBV) genome act together to co-ordinate the expression of lytic cycle genes at the majority of EBV promoters. PMID: 22022468
  30. conclude that TGF-beta induces EBV lytic reactivation via the canonical Smad pathway by activating BZLF1 gene expression through multiple SBEs acting in concert PMID: 21593157
  31. study reports that specific single missense mutations within the basic domain of ZEBRA induce the formation of nuclear aggresomes, which strongly sequester the mutant protein PMID: 21233201
  32. The authors conclude that the ZIIR element is a potent silencing element in the BZLF1 promoter Zp and plays a key role in establishment and maintenance of virus latency by inhibiting activation of Zp through the PKC signal transduction pathway. PMID: 21389123
  33. This study also reveals that the interaction between Zta and Ku80 involves the C-terminal region of Zta and the 425 aa N-terminal region of Ku80. PMID: 21123545
  34. Data show that Daudi cells express glucocorticoid receptors that mediate Dex-dependent up-regulation of BZLF1 mRNA levels. PMID: 20466055
  35. By a chromatin immunoprecipitation (ChIP) assay, the authors show that Ubn-1 blocks EB1-human herpesvirus 4 DNA interaction. PMID: 21084479
  36. These observations indicate that the EBV lytic transactivator protein Zta displays activity consistent with a pathogenic role in pulmonary fibrosis associated with herpesvirus infection. PMID: 20817778
  37. Results strongly suggest that oxidative stress contributes to the reactivation of EBV lytic cycle, through induction of BZLF-1 gene expression. PMID: 19898754
  38. Enhanced binding of ZEBRA to the origin of lytic DNA replication is crucial for lytic viral DNA replication. PMID: 20808903
  39. Data propose that EBV protein Zta activates SOCS3 protein as an immune escape mechanism that both suppresses optimal IFNalpha secretion by human monocytes and favors a state of type I IFN irresponsiveness in these cells. PMID: 20689596
  40. sumoylation of BZLF1 regulates its transcriptional activity through histone modification during Epstein-Barr virus productive replication PMID: 20516063
  41. Study renders ZEBRA-MD a promising candidate for therapeutic protein delivery applications. PMID: 20385549
  42. These data suggest that Zta acts by inducing and/or stabilizing a DNA hairpin structure during productive infection. PMID: 20444899
  43. viral BZLF1 protein is crucial both to establish latency and to escape from it; Epstein Barr virus has evolved to its host mode of dna methylation. PMID: 20080764
  44. Our work implies that the balance of promyelocytic leukemia protein and BZLF1 levels in cells may affect how each protein functions. PMID: 16307818
  45. there are three phosphorylation sites on the Epstein-Barr virus ZEBRA protein PMID: 16321978
  46. Results describe the crystal structure of ZEBRA's DNA binding domain bound to an Epstein-Barr virus lytic gene promoter element. PMID: 16483937
  47. Comparison among viral orthologues of Zta suggest that a conserved N-terminal extension of the consensus B-ZIP domain is required for this multivalent DNA recognition capacity of Zta and is essential for viral reactivation.[Zta] PMID: 16971443
  48. BZLF1 is expressed as an immediate-early gene following primary EBV infection of B lymphocytes. PMID: 17079287
  49. We report that the plasma cell differentiation factor, XBP-1s, activates the expression of the master regulator of EBV lytic activation, BZLF1. PMID: 17898050
  50. HHV8-RTA and EBV-BZLF1 are co-localized and physically interact with each other in dually-infected PELs, required for the mutual inhibition of the two molecules PMID: 18253508

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Subcellular Location Host nucleus.
Protein Families BZIP family
Database Links

KEGG: vg:3783744

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