Recombinant Escherichia coli Ribonuclease BN (rbn)

Code CSB-YP364228ENV
MSDS
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Source Yeast
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Code CSB-EP364228ENV
MSDS
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Source E.coli
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Code CSB-EP364228ENV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP364228ENV
MSDS
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Source Baculovirus
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Code CSB-MP364228ENV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
rbn
Uniprot No.
Alternative Names
rbn; elaC; rnz; b2268; JW2263; Ribonuclease BN; RNase BN; EC 3.1.-.-; Ribonuclease Z homolog; RNase Z homolog
Species
Escherichia coli (strain K12)
Expression Region
1-305
Target Protein Sequence
MELIFLGTSA GVPTRTRNVT AILLNLQHPT QSGLWLFDCG EGTQHQLLHT AFNPGKLDKI FISHLHGDHL FGLPGLLCSR SMSGIIQPLT IYGPQGIREF VETALRISGS WTDYPLEIVE IGAGEILDDG LRKVTAYPLE HPLECYGYRI EEHDKPGALN AQALKAAGVP PGPLFQELKA GKTITLEDGR QINGADYLAA PVPGKALAIF GDTGPCDAAL DLAKGVDVMV HEATLDITME AKANSRGHSS TRQAATLARE AGVGKLIITH VSSRYDDKGC QHLLRECRSI FPATELANDF TVFNV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Zinc phosphodiesterase, which has both exoribonuclease and endoribonuclease activities, depending on the nature of the substrate and of the added divalent cation, and on its 3'-terminal structure. Can process the 3' termini of both CCA-less and CCA-containing tRNA precursors. CCA-less tRNAs are cleaved endonucleolytically after the discriminator base, whereas residues following the CCA sequence can be removed exonucleolytically or endonucleolytically in CCA-containing molecules. Does not remove the CCA sequence. May also be involved in the degradation of mRNAs. In vitro, hydrolyzes bis(p-nitrophenyl)phosphate and thymidine-5'-p-nitrophenyl phosphate.
Gene References into Functions
  1. These data indicate that RNase BN plays an important role in determining levels of the global transcription regulator, 6S RNA, throughout the growth cycle. PMID: 27875308
  2. metal selectivity of ZiPD only partially relies on first shell metal ligands PMID: 15324305
  3. A ZiPD exosite is identified that guides physiological substrate recognition in the ZiPD/ElaC protein family. PMID: 15699034
  4. the RNase Z homologue encoded by Escherichia coli elaC gene is RNase BN PMID: 15764599
  5. the first crystal structure of ZiPD determined by multiple anomalous diffraction at a resolution of 2.9 A PMID: 16452444
  6. These results suggest a potentially important new role for the RNase Z family of proteins in RNA metabolism, particularly in organisms lacking RNase E. PMID: 16629673

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Protein Families
RNase Z family, RNase BN subfamily
Database Links

KEGG: ecj:JW2263

STRING: 316407.85675333

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