Recombinant Gadus morhua Trypsin-1

Code CSB-YP018811GER
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP018811GER-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018811GER
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP018811GER
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Uniprot No.
Alternative Names
; Trypsin-1; EC 3.4.21.4; Trypsin I
Species
Gadus morhua (Atlantic cod)
Expression Region
20-241
Target Protein Sequence
I VGGYECTKHS QAHQVSLNSG YHFCGGSLVS KDWVVSAAHC YKSVLRVRLG EHHIRVNEGT EQYISSSSVI RHPNYSSYNI NNDIMLIKLT KPATLNQYVH AVALPTECAA DATMCTVSGW GNTMSSVADG DKLQCLSLPI LSHADCANSY PGMITQSMFC AGYLEGGKDS CQGDSGGPVV CNGVLQGVVS WGYGCAERDH PGVYAKVCVL SGWVRDTMAN Y
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

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 Q&A
Q:

I would like to get some informations for cod trypsin I.

A:
Thanks for your inquiry. This protein is available. The detail is as follow:
Recombinant Gadus morhua Trypsin-1
CSB-YP018811GER >> Yeast
CSB-EP018811GER >> E.coli
CSB-BP018811GER >> Baculovirus
CSB-MP018811GER >> Mammalian cell
Express Region:20-241aa ;mature full length protein.
Tag information:EP,BP,MP:N-terminal 10xHis-tagged and C-terminal Myc-tagged;YP:N-terminal 6xHis-tagged
Express Sequence:

IVGGYECTKHSQAHQVSLNSGYHFCGGSLVSKDWVVSAAHCYKSVLRVRLGEHHIRVNEGTEQYISSSSVIRHPNYSSYNINNDIMLIKLTKPATLNQYVHAVALPTECAADATMCTVSGWGNTMSSVADGDKLQCLSLPILSHADCANSYPGMITQSMFCAGYLEGGKDSCQGDSGGPVVCNGVLQGVVSWGYGCAERDHPGVYAKVCVLSGWVRDTMANY


Pls check if this protein can meet your request.
I'm afraid our protein are not GMP, HACCP certificated, but we have standard SOP and the good working habit. We are cooperation with a lot of pharmaceutical companies.
This protein is for research use only, can't be used for food.
Q:

The sequence is the wanted. I have some more questions and comments:
1.What is the activity of the trypsin e.g USP units / mg protein?
2.Can you supply large amount of active trypsin e.g in gram to kilogram scale?

A:
The answers are as follow:
1.What is the activity of the trypsin e.g USP units / mg protein? I'm afraid, we haven't test the activity of this protein.
2.Can you supply large amount of active trypsin e.g in gram to kilogram scale?
We have this ability, it depends on the protein request.

Target Background

Subcellular Location
Secreted, extracellular space.
Protein Families
Peptidase S1 family
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