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The production of this Recombinant H. pylori C17orf99 protein began at the genetic level, where the coding sequence for the C17orf99 protein was first isolated and cloned into an expression plasmid vector. Recombinant DNA technology was used in the process. Next step was cloning. The expression vector must be introduced into the host cell (Baculovirus) so that the cells could be cultured and expressed the desired ftnA protein. And we finally got the recombinant ftnA protein with the purity of 85%+ determined by SDS-PAGE.Ferritin A (FtnA), a major iron storage protein in E. coli, can scavenge the iron released from the disrupted iron–sulfur clusters and alleviates the iron-mediated production of hydroxyl free radicals in the presence of hydrogen peroxide. Ferritin minerals are nutritive iron concentrates that slowly release the iron for use in the synthesis of new iron centers for protein catalysts as in heme, iron–sulfur clusters, or iron bound entirely by protein in amino acid side chains (“non-heme iron”). Iron-containing proteins are key to electron transfer chains in respiration (heme proteins) and photosynthesis (iron–sulfur proteins) and play important roles in hydroxylation reactions. The iron stored in FtnA can be retrieved by IscA for the iron–sulfur cluster assembly in IscU in the presence of the thioredoxin reductase system. The physiological roles of ferritins in cellular response to oxidative stresses and in repair of the disrupted iron–sulfur clusters have been identified. Increasing evidence indicated that ferritins, a large family of iron-storage proteins found in bacteria and mammalian cells may have an important role in cellular defense against oxidative stresses.
|Purity||Greater than 85% as determined by SDS-PAGE.|
ftnA; pfr; HP_0653Bacterial non-heme ferritin; EC 184.108.40.206
|Species||Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)|
|Target Protein Sequence||MLSKDIIKLLNEQVNKEMNSSNLYMSMSSWCYTHSLDGAGLFLFDHAAEEYEHAKKLIVFLNENNVPVQLTSISAPEHKFEGLTQIFQKAYEHEQHISESINNIVDHAIKGKDHATFNFLQWYVSEQHEEEVLFKDILDKIELIGNENHGLYLADQYVKGIAKSRKS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
|Mol. Weight||21.8 kDa
|Protein Length||Full Length|
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
|Buffer|| If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
|Reconstitution||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.|
|Storage Condition||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.|
|Shelf Life||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
|Lead Time||3-7 business days|
|Notes||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.|
|Datasheet & COA||Please contact us to get it.|
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|Protein Families||Ferritin family, Prokaryotic subfamily|