Recombinant Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 (PLCH2), partial

Code CSB-YP018137HU
MSDS
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Source Yeast
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Code CSB-EP018137HU
MSDS
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Source E.coli
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Code CSB-EP018137HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018137HU
MSDS
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Source Baculovirus
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Code CSB-MP018137HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PLCH2
Uniprot No.
Alternative Names
1-phosphatidylinositol-4; 5-bisphosphate phosphodiesterase eta-2; KIAA0450; Phosphoinositide phospholipase C-eta-2; Phosphoinositide phospholipase C-like 4; Phospholipase C-eta-2; Phospholipase C-like protein 4; PLC-eta2; PLC-L4; Plch2; PLCH2_HUMAN; PLCL4
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This phospholipase activity is very sensitive to calcium. May be important for formation and maintenance of the neuronal network in the postnatal brain.
Gene References into Functions
  1. PLCH2 might be a putative candidate gene for the neurodevelopmental delay observed in patients bearing 1p36.3 deletions. PMID: 21474938
  2. The researchers investigated the D1S80 minisatellite and associated single nucelotide polymorphisms in a Tamil population in India. PMID: 20649401
  3. molecular cloning of PLC-eta1 and PLC-eta2 PMID: 15583837
  4. PLC(eta)2 is a neuron-specific isozyme that may be important for the formation and/or maintenance of the neuronal network in the postnatal brain PMID: 15899900
  5. The molecular cloning and sequencing of phospholipase C eta2 are reported. PMID: 16107206
  6. Purified Gbeta 1gamma 2 stimulated the activity of purified PLC-eta2 in a concentration-dependent manner similar to that observed with purified PLC-beta2. PMID: 18361507

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Subcellular Location
Cytoplasm. Cell membrane.
Tissue Specificity
Expressed in retina and kidney.
Database Links

HGNC: 29037

OMIM: 612836

KEGG: hsa:9651

STRING: 9606.ENSP00000367747

UniGene: Hs.170156

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