Name: Recombinant Human Casein kinase I isoform alpha (CSNK1A1)
Brand: CUSABIO
SKU: CSB-EP006065HU

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

CSNK1A1

Uniprot No.

P48729

Research Area

Cell Cycle

Alternative Names

Casein kinase 1 alpha 1; Casein kinase I isoform alpha; CK1; CK1A; CKI alpha; CKI-alpha; CKIa; Clock regulator kinase; Csnk1a1; Down regulated in lung cancer ; HLCDGP1; KC1A_HUMAN; PRO2975

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

2-337aa

Target Protein Sequence

ASSSGSKAEFIVGGKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQKARHPQLLYESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDLFNFCSRRFTMKTVLMLADQMISRIEYVHTKNFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTMLKQKAAQQAASSSGQGQQAQTPTGKQTDKTKSNMKGF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

42.8kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

I am interested in purchasing 1mg of your product CSB-EP006065HU. However, they would like it to be Avi-tagged. Is this possible? If so, can you answer the following questions:
1.What are the recommended storage conditions?
2.Do you have a special ID or catalog number for this item?

Very nice to receive your inquiry. The replies for your questions are as below.
1. What are the recommended storage conditions?
The recommended storage buffer is Tris-based buffer, 50% glycerol. For short-term storage, store at 4℃. For long-term storage, store at -20℃ or -80℃.
2. Do you have a special ID or catalog number for this item?
The special catalog number for this item is CSB-EP006065HUg2.
The protein we provide is non-biotinylated protein. If you want the biotinylated protein, they need to use externally purchased enzymes or kits for biotinylation in vitro.
Reference Link: https://www.avidity.com/avitag/bira-500-bira-biotin-protein-ligase-standard-reaction-kit

Target Background

Function

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration.

Gene References into Functions

Study reports that CK1alpha similarly destabilizes FOXO4 in RAS-mutant cells by phosphorylation at serines 265/268. PMID: 28945225
Data indicate that casein kinase 1 alpha (CK1alpha) RNA levels might serve as prognostic marker for colorectal cancer (CRC). PMID: 29409464
cellular kinase, casein kinase 1alpha (CK1alpha), is crucial for IAV HA-induced degradation of both IFNGR1 and IFNAR1. PMID: 29343571
casein kinase 1 phosphorylates the SQSTM1 S349 residue when harmful proteins accumulate under HSF1 stress PMID: 27846364
Maintenance of the progenitors thus requires cooperation by PRMT1 and CSNK1a1 to sustain proliferation gene expression and suppress premature differentiation driven by GRHL3 PMID: 28943242
These data indicate that CK1alpha has a dominant and non-redundant function in melanoma cells PMID: 27488834
Disruption of CK1alpha function in myeloma cells resulted in decreased Mdm2, increased p53 and p21 and reduced expression of beta-catenin and AKT. PMID: 28099937
CK1delta enhances EPO secretion from liver cancer cells under hypoxia by modifying HIF-2alpha and promoting its nuclear accumulation. PMID: 27686097
CKIalpha-mediated NS5A S235 phosphorylation is critical for HCV replication. CaMKII gamma and delta may have negative roles in the HCV life cycle. PMID: 27875595
Interactome analyses revealed that the Jade-1S mutant unable to be phosphorylated by CK1alpha has an increased binding affinity to proteins involved in chromatin remodelling, histone deacetylation, transcriptional repression, and ribosome biogenesis. PMID: 26919559
study focuses on, how the structural dynamics and conformational changes of two CK1 isoforms are synchronized in carcinogenic pathway PMID: 26788877
NIFK is required for lung cancer progression via the RUNX1-dependent CK1alpha repression, which activates TCF4/beta-catenin signaling in metastasis and the Ki-67-dependent regulation in cell proliferation. PMID: 26984280
The study describes the identification of a novel, tumor-specific missense mutation in the active site of casein kinase 1 alpha(CSNK1A1) using activity-based proteomics. PMID: 27031502
characterization of frequency and potential clinical impact of CSNK1A1 mutations in myelodysplastic syndrome(MDS) and acute myeloid leukemia following MDS in a large cohort of patients PMID: 25792355
The dynamical and conformational properties for each of three isoforms of CK1 are explored through molecular dynamics (MD) simulations. PMID: 25665722
Phosphorylation of LRRK2 by casein kinase 1alpha regulates trans-Golgi clustering via differential interaction with ARHGEF7. PMID: 25500533
Casein kinase 1alpha is a key negative regulator of oncogenic RAS-induced autophagy. Depletion or pharmacologic inhibition of CK1alpha enhanced autophagic flux in oncogenic RAS-driven human fibroblasts and multiple cancer cell lines. PMID: 25798617
CSNK1alpha1 has a role in mediating malignant plasma cell survival PMID: 24962017
Casein kinase 1 alpha specifically phosphorylates Jade-1 at an unprimed SLS phosphorylation site. PMID: 25100726
Degradation of Tiam1 by casein kinase 1 and the SCFbetaTrCP ubiquitin ligase controls the duration of mTOR-S6K signaling. PMID: 25124033
Data indicate that protein S phosphorylation by casein kinase 1 (CK1) and casein kinase 2 (CK2) increased activated proten C cofactor activity. PMID: 23580615
The substrate interacting residues in ck1alpha have been identified using the structural model of kinase - substrate peptide. The PKR interacting NS5A 1b residues have also been predicted. PMID: 23148689
DNA damage activates p53 in part by disrupting CK1a-MDMX interaction and reducing MDMX-p53 binding affinity. PMID: 23028042
identified casein kinase 1alpha (CK1alpha) as a direct target of miR-155 control which enhanced beta-catenin signaling and cyclin D1 expression, promoting tumor cell growth PMID: 22350414
We showed that just four genes, G3BP2, SCARB2, CSNK1A1 and SPRR2B, can classify patients as presence of lymph node metastasis negative or positive, with 80.0% accuracy. PMID: 21985131
mTOR cooperates with CK1alpha and betaTrCP to generate an auto-amplification loop to promote its own full activation. PMID: 22017877
The authors characterized the role of CKIalpha in the clock mechanism and found that CKIalpha-mediated phosphorylation stimulated degradation of a clock protein PER1, similar to the function of CKIdelta. PMID: 21179498
Our results indicate that the nuclear form of CK1alpha in humans, CK1alphaLS, plays a critical role in vascular cell proliferation, cellular activation, and hydrogen peroxide-mediated mitogenic signal transduction. PMID: 20696773
Casein kinase 1 functions as both penultimate and ultimate kinase in regulating Cdc25A destruction. PMID: 20348946
Biochemical and cellular characteristics of the four splice variants of protein kinase CK1alpha from zebrafish (Danio rerio)are presented. PMID: 12210746
CK1alpha can promote cell survival by interfering with RXR agonist-induced apoptosis PMID: 15131121
CK1alpha-mediated phosphorylation modulates the mRNA binding ability of hnRNP-C PMID: 15687492
NFAT1, a transcription factor connected with breast cancer metastasis, is activated by Wnt-5a through a Ca2+ signaling pathway in human breast epithelial cells which was simultaneously counteracted by a Wnt-5a-induced Yes/Cdc42 signaling pathway. PMID: 16880514
identification of the alpha isoform of casein kinase I as the cellular kinase important for the formation of hepatitis C virus (HCV) NS5A-p58; here we show the effects of this kinase on NS5A hyperphosphorylation and HCV replication PMID: 16943283
CK1alphaLS and hnRNP-C represent conserved components of a vertebrate-specific H(2)O(2)-responsive nuclear signaling pathway. PMID: 17626781
Studies suggest that CK1alpha is another kinase that regulates RIP1 function in NF-kappaB activation. PMID: 18067272
following DNA damage, PML facilitates Thr18 phosphorylation by recruiting p53 and CK1 into PML nuclear bodies, thereby protecting p53 from inhibition by Mdm2, leading to p53 activation. PMID: 18246126
Ser-155 and Ser-158 have important roles in the nucleocytoplasmic trafficking of OREBP/TonEBP and CK1 plays a major role in regulating this process PMID: 18411282
We show that the inhibition of CK1 activates RhoB and promotes RhoB dependent actin fiber formation and EGF-R level. PMID: 18590726
CK1 has a role as the Ser20 site kinase for p53 in DNA virus-infected cells PMID: 18669630
Phosphorylation of parkin by casein kinase I and cdk5 decreases parkin solubility, leading to its aggregation and inactivation. PMID: 19050041
'activated B-cell-like' subtype of diffuse large B-cell lymphoma cells required CK1alpha for constitutive NF-kappaB activity, indicating that CK1alpha functions as a conditionally essential malignancy gene PMID: 19118383
Casein Kinase 1 phosphorylates serine 56 of yellow fever virus methyltransferase. PMID: 19185594
Data show that Wnt-5a stimulation of breast epithelial cells leads to increased cell-cell adhesion, and that Wnt-5a/casein kinase Ialpha (CKIalpha)-specific phosphorylation of beta-catenin leads to increased complex formation of beta-catenin/E-cadherin. PMID: 19244247
CK1alpha plays a central role in mediating MDM2 control of p53 and E2F-1 protein stability PMID: 19759023
Results suggest a role for mammalian and parasite casein kinase 1alpha in regulating IFNAR1 stability and type I IFN signaling. PMID: 19805514

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Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Nucleus speckle. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, spindle.

Protein Families

Protein kinase superfamily, CK1 Ser/Thr protein kinase family, Casein kinase I subfamily

Database Links

HGNC: 2451

OMIM: 600505

KEGG: hsa:1452

UniGene: Hs.529862

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Code	CSB-EP006065HU
Abbreviation	Recombinant Human CSNK1A1 protein
MSDS	MSDS Datasheet
Size	$224
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP006065HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) CSNK1A1. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP006065HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) CSNK1A1.
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Recombinant Human Casein kinase I isoform alpha (CSNK1A1)

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