Recombinant Human Cytochrome P450 1A1(CYP1A1)

Code CSB-EP006395HUb0
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names CYP1A1
Uniprot No. P04798
Research Area Cancer
Alternative Names AHH; AHRR; Aryl hydrocarbon hydroxylase; CP11; CP1A1_HUMAN; CYP 1; CYP1; cyp1a1; CYPIA1; Cytochrome P1 450 dioxin inducible; Cytochrome P1-450; Cytochrome P450 1A1; Cytochrome P450 family 1 subfamily A polypeptide 1; Cytochrome P450 form 6; Cytochrome P450 subfamily I (aromatic compound inducible) polypeptide 1; Cytochrome P450-C; Cytochrome P450-P1; Flavoprotein-linked monooxygenase; Microsomal monooxygenase; P1 450; P450 C; P450 form 6; P450 P1; P450DX; Xenobiotic monooxygenase
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-512aa
Target Protein Sequence MLFPISMSATEFLLASVIFCLVFWVIRASRPQVPKGLKNPPGPWGWPLIGHMLTLGKNPHLALSRMSQQYGDVLQIRIGSTPVVVLSGLDTIRQALVRQGDDFKGRPDLYTFTLISNGQSMSFSPDSGPVWAARRRLAQNGLKSFSIASDPASSTSCYLEEHVSKEAEVLISTLQELMAGPGHFNPYRYVVVSVTNVICAICFGRRYDHNHQELLSLVNLNNNFGEVVGSGNPADFIPILRYLPNPSLNAFKDLNEKFYSFMQKMVKEHYKTFEKGHIRDITDSLIEHCQEKQLDENANVQLSDEKIINIVLDLFGAGFDTVTTAISWSLMYLVMNPRVQRKIQEELDTVIGRSRRPRLSDRSHLPYMEAFILETFRHSSFVPFTIPHSTTRDTSLKGFYIPKGRCVFVNQWQINHDQKLWVNPSEFLPERFLTPDGAIDKVLSEKVIIFGMGKRKCIGETIARWEVFLFLAILLQRVEFSVPLGVKVDMTPIYGLTMKHACCEHFQMQLRS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 61.8 kDa
Protein Length Full Length
Tag Info N-terminal 10xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15-alpha and C16-alpha positions. Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation. Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system. Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer. May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent).
Gene References into Functions
  1. Results indicate that the underlying mechanisms whereby etoposide and ellipticine regulate CYP1A1 expression must be different and may not be linked to p53 activation alone. PMID: 29471073
  2. rs2470893 SNP, which maps 196 bp from a CYP1A1 promoter XRE, is associated with variations in 3MC-dependent AHR binding and CYP1A1 expression. PMID: 29980579
  3. study demonstrates the importance of CYP1A1 gene expression as well as organochlorine pesticides levels in the context of urinary bladder cancer risk. PMID: 29027484
  4. The study revealed association of CYP1A1 I462V with Infectious Endocarditis. PMID: 30289676
  5. Differential methylation was observed in the CYP1A1 promoter and AHRR gene body regions between women who smoked throughout pregnancy and non-smokers on the fetal-side of the placenta and in the GFI1 promoter between women who quit smoking while pregnant and non-smokers on the maternal-side of the placenta PMID: 28820654
  6. Our meta-analysis supports that CYP1A1 exon7 polymorphism might contribute to individual susceptibility to esophageal cancer in the Chinese population. Gene-gene interaction analysis found a synergistic interaction between CYP1A1 mutation genotypes and GSTM1 deletion genotype. PMID: 28164573
  7. For CYP1A1, exposure to maternal smoking was not associated with fetal DNA methylation changes whereas mRNA expression increased in placentas and male fetal livers. PMID: 29169084
  8. SNP variants of CYP1A1, rs1048943 (A4889G), rs4646903 (T3801C), were typed in Mexican women with breast cancer and normal controls. There was no significant association. PMID: 29267651
  9. Study shows the effect of dietary flavonoid intake on colorectal cancer risk differs according to flavonoid subclasses and CYP1A1 genetic variants. PMID: 28273931
  10. CYP1A1 mRNA expression in the preputial tissues from patients with hypospadias and phimosis PMID: 29080015
  11. The association between persistent exposure to PM10 and lung function decline in Korean men may be determined in part by several functional variants of the CYP1A1 gene. PMID: 29627601
  12. These observations reveal a regulatory role of CYP1A1 in liver lipid peroxidation and imply CYP1A1 as a potential therapeutic target. PMID: 29738693
  13. genetic association studies in population of postmenopausal Mexican-Mestizo women: Data suggest SNPs in CYP1A1 (rs1799814, rs1048943; but not rs1056836 in CYP1B1) are associated with postmenopausal osteoporosis in the population studied; SNPs in CYP1A1 are associated with low bone mineral density in lumbar spine and femur neck. (CYP1A1 = aryl hydrocarbon hydroxylase CYP1A1; CYP1B1 = aryl hydrocarbon hydroxylase CYP1B1) PMID: 28300467
  14. Meta-analysis showed that there was an interaction between the CYP1A1 MspI and smoking on the risk of lung cancer in the Chinese population. [Meta-Analysis] PMID: 26918783
  15. The SNPs in CYP1A1, GSTP1 and XRCC1 genes did not show significant association with complete remission (CR) rate, overall survival (OS) or event free survival (EFS). However, XRCC1 Arg194Trp SNP was associated with higher drug toxicity; carriers of variant genotypes (CT and TT) had a significantly higher frequency of myelosuppression compared to those with the wild CC genotype PMID: 28844589
  16. Our findings revealed that I3C inhibits the proliferation of THP-1 cells in a dose- and time-dependent manner with minimal toxicity over normal monocytes. The AhR target genes (CYP1A1, IL1beta) were overexpressed upon I3C treatment PMID: 28812970
  17. Data suggest that response of bronchial epithelial cells to environmental carcinogen benzo[a]pyrene includes activation of AhR/Src/ERK signaling, CYP1A1 induction, and formation of stable DNA adducts. (AhR = aryl hydrocarbon receptor; Src = Src proto-oncogene kinase; ERK = extracellular signal-regulated kinases; CYP1A1 = cytochrome P450 family 1 subfamily A member 1) PMID: 29545172
  18. This meta-analysis demonstrated that polymorphisms in MspI and Ile462Val of CYP1A1 were risk factors for developing cervical cancer. PMID: 29595663
  19. Computational molecular modeling tools have been used by several research groups to decipher the specificity and potency factors of the CYP1A1 and CYP1A2 substrates. In this review, we perform a thorough analysis of the computational studies that are ligand-based and protein-ligand complex-based to catalog the various factors that govern the specificity/potency toward these two enzymes PMID: 28698457
  20. Results showed that polymorphism in PPARGrs10865710, PPARGrs1805192 and CYP1A1rs4646903 and interaction between PPARGrs1805192 and CYP1A1rs4646903 were related with increased coronary artery disease susceptibility. PMID: 28415751
  21. The increased risk of prostate cancer was observed among individuals with the CYP1A1 GA + GG genotype. CYP1A1 GA + GG genotypes were associated with increased cancer risk in low (OR, 2.05; 95% CI, 1.19-3.63), moderate (OR, 1.72; 95% CI, 1.07-2.76), and high (OR, 2.86; 95% CI, 1.83-4.47) HAA intake groups. PMID: 29165164
  22. rs4886605 and rs4646903 are associated with increased CAD risk, but rs1048943 is associated with decreased CAD risk; we also found gene-gene interaction between rs4886605 and rs4646903 and gene-environment interaction between rs4886605 and smoking. PMID: 28513235
  23. CYP1A1 has an oncogenic role in prostate cancer via promoter hypomethylation that is influenced by tobacco smoking PMID: 27203547
  24. Utilising a three-dimensional lymph endothelial cell (LEC) monolayer & MDA-MB231 breast cancer cell spheroid co-culture model in combination with knock-down approach allowed elucidation of the molecular/biochemical basis of AHR/CYP1A1-induced tumour breaching through the LEC barrier. PMID: 28171546
  25. This meta-analysis demonstrated CYP1A1 rs1048943 A>G may increase the susceptibility to colorectal cancer (CRC) instead of rs4646903 T> C. This conclusion suggested CYP1A1 may contribute to the pathogenesis of CRC. [meta-analysis] PMID: 27384991
  26. The Rs4646903 polymorphism of CYP1A1 is Associated with Recurrent Pregnancy Loss, especially among South Asians. PMID: 28620991
  27. We demonstrated that infants whose mothers smoked during pregnancy with the combination of AHR, CYP1A1, and XRCC1 polymorphisms had lower birth size. PMID: 27592400
  28. genetic variations in STX1B, DNMT3A and CYP1A1 have roles in influencing warfarin maintenance dose PMID: 27740732
  29. CYP1A1 rs2606345 influences response to anticonvulsants in North Indian women with epilepsy by lowering CYP1A1 expression. PMID: 26951882
  30. This is the first report providing evidence of hyperoxia-mediated transcriptional activation of the human CYP1A1 promoter in newborn mice, and this in conjunction with decreased lung injury, suggests that these phenomena have important implications for bronchopulmonary dysplasia. PMID: 29101037
  31. Review/Meta-analysis: CYP1A1 T3801C and A2455G variants might not be risk alleles for breast cancer susceptibility in Chinese individuals. PMID: 28164497
  32. These findings identify a role for pattern recognition receptor (PRR) signaling in regulating the AHR response through selective down-regulation of Cyp1 expression in human monocytes and macrophages. PMID: 29018148
  33. AHR, CYP1A1, CYP1A2, or CYP1B1 variants associated with head-and-neck squamous cell carcinoma risk in smokers. PMID: 27894333
  34. Our meta-analysis revealed strong association of rs1048943 in CYP1A1, but a suggestive association of deletion polymorphisms in GSTT1 and GSTM1 with lung cancer, which provides a comprehensive insight on the overall effect of the polymorphic variants, reported in various case-control studies on Indian population, on the risk of lung cancer development PMID: 29076184
  35. Increased expression of CYP1A1 is associated with ovarian/peritoneal endometriotic lesions. PMID: 27012269
  36. CYP1A1m2 polymorphism showed an increased risk in the population leading to cervical cancer PMID: 28433806
  37. mtDNA content, methylation of specific loci of mtDNA, and CYP1A1 methylation in placental tissue may serve as molecular signatures for the association between gestational tobacco smoke exposure and low birth weight. PMID: 28052772
  38. Studied diosmetin effect on signal pathway and apoptosis in HepG2 cells. Dios-induced cell apoptosis could be reversed by p53 blockade and the effects of Dios on cellular P53 and CYP1A1/CYP1A2 proteins levels were examined. Found P53, CYP1A1, and CYP1A2 proteins were up-regulated by diosmetin; p53 downregulation by pifithrin caused inhibition of apoptosis. PMID: 28245756
  39. individuals with CYP1A1m13801CC and 3801TC genotypes independently and in the presence of alcohol and nevirapine usage is likely to be associated with increased risk of development of antiretroviral-associated hepatotoxicity, its severity, and advancement of disease PMID: 28370504
  40. the CYP1A1 polymorphisms are potential risk factors for digestive tract cancer. PMID: 27061602
  41. was observed that the variant genotypes of GSTM1, GSTT1, GSTP1 and CYP1A1 did not significantly increase the risk of cervical cancer (CC), however, statistically significant increased risk was observed for women who used wood for cooking and had GSTM1 (null) genotype. PMID: 28361858
  42. CYP1A1*2A was not associated with Colorectal Cancer(CRC)development (OR = 1.566; 95% confidence interval [CI] = 0.90-2.73; p = 0.12); C allele-carrying individuals who had smoked within the past 5 years had a significant risk of CRC (OR = 2.28; 95% CI = 1.07-4.86; p = 0.043). PMID: 27243113
  43. CYP1A1 polymorphisms were not associated with sporadic colorectal neoplasms. PMID: 28018104
  44. Allele frequency of the CYP1A1 3801T>C polymorphism does not correlate with semen production as determined by sperm counts or sperm function as determined by fertilization rates with ICSI. PMID: 27117375
  45. data indicate that CYP1A1 I462V allele confers the least carcinogen-associated genotoxicity, compared to CYP1A1; however, results vary depending on the chemical carcinogen and the genotoxic endpoint PMID: 28283091
  46. By sequencing Apc and Ctnnb1 genes, we found that most PhIP-induced small intestinal tumors in obese mice carried only a single heterozygous mutation in Apc . Our findings demonstrate that PhIP-induced small intestinal carcinogenesis in hCYP1A-db/db mice is promoted by obesity and involves Apc mutation and inactivation by DNA hypermethylation PMID: 27207656
  47. The rapid activation of the Ah receptor and CYP1A1 protein expression by higher chlorinated polychlorinated naphthalene point to their toxicity; however, it is not sufficient for potential carcinogenicity. PMID: 27501765
  48. The polymorphism of CYP1A1*4 is associated with esophageal squamous cell carcinoma risk. PMID: 26455829
  49. Our results showed that 3 single nucleotide polymorphisms of ACE rs4340, IL-6 rs1800795, and NOS3 rs1799983 were significantly associated with the risks of mycoplasma pneumoniae pneumonia (MPP), while no differences were observed in genotype frequencies of GSTM1 and CYP1A1. The combinations of ACE/NOS3/CYP1A1 and ACE /NOS3 contribute to the genetic susceptibility of MPP in Chinese children. PMID: 28403117
  50. the CYP1A1 (rs4646422) polymorphism might be involved in gastric carcinogenesis among the Japanese population. PMID: 27062139

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Subcellular Location Endoplasmic reticulum membrane; Peripheral membrane protein. Mitochondrion inner membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Cytoplasm.
Protein Families Cytochrome P450 family
Tissue Specificity Lung, lymphocytes and placenta.
Database Links

HGNC: 2595

OMIM: 108330

KEGG: hsa:1543

STRING: 9606.ENSP00000369050

UniGene: Hs.72912

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