Recombinant Human Cytochrome P450 1A1(CYP1A1)

Code CSB-YP006395HU
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Source Yeast
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Code CSB-EP006395HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006395HU
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Source Baculovirus
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Code CSB-MP006395HU
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Source Mammalian cell
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names CYP1A1
Uniprot No. P04798
Research Area Cancer
Alternative Names AHH; AHRR; Aryl hydrocarbon hydroxylase; CP11; CP1A1_HUMAN; CYP 1; CYP1; cyp1a1; CYPIA1; Cytochrome P1 450 dioxin inducible; Cytochrome P1-450; Cytochrome P450 1A1; Cytochrome P450 family 1 subfamily A polypeptide 1; Cytochrome P450 form 6; Cytochrome P450 subfamily I (aromatic compound inducible) polypeptide 1; Cytochrome P450-C; Cytochrome P450-P1; Flavoprotein-linked monooxygenase; Microsomal monooxygenase; P1 450; P450 C; P450 form 6; P450 P1; P450DX; Xenobiotic monooxygenase
Species Homo sapiens (Human)
Expression Region 1-512aa
Target Protein Sequence MLFPISMSATEFLLASVIFCLVFWVIRASRPQVPKGLKNPPGPWGWPLIGHMLTLGKNPHLALSRMSQQYGDVLQIRIGSTPVVVLSGLDTIRQALVRQGDDFKGRPDLYTFTLISNGQSMSFSPDSGPVWAARRRLAQNGLKSFSIASDPASSTSCYLEEHVSKEAEVLISTLQELMAGPGHFNPYRYVVVSVTNVICAICFGRRYDHNHQELLSLVNLNNNFGEVVGSGNPADFIPILRYLPNPSLNAFKDLNEKFYSFMQKMVKEHYKTFEKGHIRDITDSLIEHCQEKQLDENANVQLSDEKIINIVLDLFGAGFDTVTTAISWSLMYLVMNPRVQRKIQEELDTVIGRSRRPRLSDRSHLPYMEAFILETFRHSSFVPFTIPHSTTRDTSLKGFYIPKGRCVFVNQWQINHDQKLWVNPSEFLPERFLTPDGAIDKVLSEKVIIFGMGKRKCIGETIARWEVFLFLAILLQRVEFSVPLGVKVDMTPIYGLTMKHACCEHFQMQLRS
Mol. Weight 60.2kDa
Protein Length Full Length
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Gene References into Functions
  1. Results indicate that the underlying mechanisms whereby etoposide and ellipticine regulate CYP1A1 expression must be different and may not be linked to p53 activation alone. PMID: 29471073
  2. rs2470893 SNP, which maps 196 bp from a CYP1A1 promoter XRE, is associated with variations in 3MC-dependent AHR binding and CYP1A1 expression. PMID: 29980579
  3. study demonstrates the importance of CYP1A1 gene expression as well as organochlorine pesticides levels in the context of urinary bladder cancer risk. PMID: 29027484
  4. The study revealed association of CYP1A1 I462V with Infectious Endocarditis. PMID: 30289676
  5. Differential methylation was observed in the CYP1A1 promoter and AHRR gene body regions between women who smoked throughout pregnancy and non-smokers on the fetal-side of the placenta and in the GFI1 promoter between women who quit smoking while pregnant and non-smokers on the maternal-side of the placenta PMID: 28820654
  6. Our meta-analysis supports that CYP1A1 exon7 polymorphism might contribute to individual susceptibility to esophageal cancer in the Chinese population. Gene-gene interaction analysis found a synergistic interaction between CYP1A1 mutation genotypes and GSTM1 deletion genotype. PMID: 28164573
  7. For CYP1A1, exposure to maternal smoking was not associated with fetal DNA methylation changes whereas mRNA expression increased in placentas and male fetal livers. PMID: 29169084
  8. SNP variants of CYP1A1, rs1048943 (A4889G), rs4646903 (T3801C), were typed in Mexican women with breast cancer and normal controls. There was no significant association. PMID: 29267651
  9. Study shows the effect of dietary flavonoid intake on colorectal cancer risk differs according to flavonoid subclasses and CYP1A1 genetic variants. PMID: 28273931
  10. CYP1A1 mRNA expression in the preputial tissues from patients with hypospadias and phimosis PMID: 29080015
  11. The association between persistent exposure to PM10 and lung function decline in Korean men may be determined in part by several functional variants of the CYP1A1 gene. PMID: 29627601
  12. These observations reveal a regulatory role of CYP1A1 in liver lipid peroxidation and imply CYP1A1 as a potential therapeutic target. PMID: 29738693
  13. genetic association studies in population of postmenopausal Mexican-Mestizo women: Data suggest SNPs in CYP1A1 (rs1799814, rs1048943; but not rs1056836 in CYP1B1) are associated with postmenopausal osteoporosis in the population studied; SNPs in CYP1A1 are associated with low bone mineral density in lumbar spine and femur neck. (CYP1A1 = aryl hydrocarbon hydroxylase CYP1A1; CYP1B1 = aryl hydrocarbon hydroxylase CYP1B1) PMID: 28300467
  14. Meta-analysis showed that there was an interaction between the CYP1A1 MspI and smoking on the risk of lung cancer in the Chinese population. [Meta-Analysis] PMID: 26918783
  15. The SNPs in CYP1A1, GSTP1 and XRCC1 genes did not show significant association with complete remission (CR) rate, overall survival (OS) or event free survival (EFS). However, XRCC1 Arg194Trp SNP was associated with higher drug toxicity; carriers of variant genotypes (CT and TT) had a significantly higher frequency of myelosuppression compared to those with the wild CC genotype PMID: 28844589
  16. Our findings revealed that I3C inhibits the proliferation of THP-1 cells in a dose- and time-dependent manner with minimal toxicity over normal monocytes. The AhR target genes (CYP1A1, IL1beta) were overexpressed upon I3C treatment PMID: 28812970
  17. Data suggest that response of bronchial epithelial cells to environmental carcinogen benzo[a]pyrene includes activation of AhR/Src/ERK signaling, CYP1A1 induction, and formation of stable DNA adducts. (AhR = aryl hydrocarbon receptor; Src = Src proto-oncogene kinase; ERK = extracellular signal-regulated kinases; CYP1A1 = cytochrome P450 family 1 subfamily A member 1) PMID: 29545172
  18. This meta-analysis demonstrated that polymorphisms in MspI and Ile462Val of CYP1A1 were risk factors for developing cervical cancer. PMID: 29595663
  19. Computational molecular modeling tools have been used by several research groups to decipher the specificity and potency factors of the CYP1A1 and CYP1A2 substrates. In this review, we perform a thorough analysis of the computational studies that are ligand-based and protein-ligand complex-based to catalog the various factors that govern the specificity/potency toward these two enzymes PMID: 28698457
  20. Results showed that polymorphism in PPARGrs10865710, PPARGrs1805192 and CYP1A1rs4646903 and interaction between PPARGrs1805192 and CYP1A1rs4646903 were related with increased coronary artery disease susceptibility. PMID: 28415751
  21. The increased risk of prostate cancer was observed among individuals with the CYP1A1 GA + GG genotype. CYP1A1 GA + GG genotypes were associated with increased cancer risk in low (OR, 2.05; 95% CI, 1.19-3.63), moderate (OR, 1.72; 95% CI, 1.07-2.76), and high (OR, 2.86; 95% CI, 1.83-4.47) HAA intake groups. PMID: 29165164
  22. rs4886605 and rs4646903 are associated with increased CAD risk, but rs1048943 is associated with decreased CAD risk; we also found gene-gene interaction between rs4886605 and rs4646903 and gene-environment interaction between rs4886605 and smoking. PMID: 28513235
  23. CYP1A1 has an oncogenic role in prostate cancer via promoter hypomethylation that is influenced by tobacco smoking PMID: 27203547
  24. Utilising a three-dimensional lymph endothelial cell (LEC) monolayer & MDA-MB231 breast cancer cell spheroid co-culture model in combination with knock-down approach allowed elucidation of the molecular/biochemical basis of AHR/CYP1A1-induced tumour breaching through the LEC barrier. PMID: 28171546
  25. This meta-analysis demonstrated CYP1A1 rs1048943 A>G may increase the susceptibility to colorectal cancer (CRC) instead of rs4646903 T> C. This conclusion suggested CYP1A1 may contribute to the pathogenesis of CRC. [meta-analysis] PMID: 27384991
  26. The Rs4646903 polymorphism of CYP1A1 is Associated with Recurrent Pregnancy Loss, especially among South Asians. PMID: 28620991
  27. We demonstrated that infants whose mothers smoked during pregnancy with the combination of AHR, CYP1A1, and XRCC1 polymorphisms had lower birth size. PMID: 27592400
  28. genetic variations in STX1B, DNMT3A and CYP1A1 have roles in influencing warfarin maintenance dose PMID: 27740732
  29. CYP1A1 rs2606345 influences response to anticonvulsants in North Indian women with epilepsy by lowering CYP1A1 expression. PMID: 26951882
  30. This is the first report providing evidence of hyperoxia-mediated transcriptional activation of the human CYP1A1 promoter in newborn mice, and this in conjunction with decreased lung injury, suggests that these phenomena have important implications for bronchopulmonary dysplasia. PMID: 29101037
  31. Review/Meta-analysis: CYP1A1 T3801C and A2455G variants might not be risk alleles for breast cancer susceptibility in Chinese individuals. PMID: 28164497
  32. These findings identify a role for pattern recognition receptor (PRR) signaling in regulating the AHR response through selective down-regulation of Cyp1 expression in human monocytes and macrophages. PMID: 29018148
  33. AHR, CYP1A1, CYP1A2, or CYP1B1 variants associated with head-and-neck squamous cell carcinoma risk in smokers. PMID: 27894333
  34. Our meta-analysis revealed strong association of rs1048943 in CYP1A1, but a suggestive association of deletion polymorphisms in GSTT1 and GSTM1 with lung cancer, which provides a comprehensive insight on the overall effect of the polymorphic variants, reported in various case-control studies on Indian population, on the risk of lung cancer development PMID: 29076184
  35. Increased expression of CYP1A1 is associated with ovarian/peritoneal endometriotic lesions. PMID: 27012269
  36. CYP1A1m2 polymorphism showed an increased risk in the population leading to cervical cancer PMID: 28433806
  37. mtDNA content, methylation of specific loci of mtDNA, and CYP1A1 methylation in placental tissue may serve as molecular signatures for the association between gestational tobacco smoke exposure and low birth weight. PMID: 28052772
  38. Studied diosmetin effect on signal pathway and apoptosis in HepG2 cells. Dios-induced cell apoptosis could be reversed by p53 blockade and the effects of Dios on cellular P53 and CYP1A1/CYP1A2 proteins levels were examined. Found P53, CYP1A1, and CYP1A2 proteins were up-regulated by diosmetin; p53 downregulation by pifithrin caused inhibition of apoptosis. PMID: 28245756
  39. individuals with CYP1A1m13801CC and 3801TC genotypes independently and in the presence of alcohol and nevirapine usage is likely to be associated with increased risk of development of antiretroviral-associated hepatotoxicity, its severity, and advancement of disease PMID: 28370504
  40. the CYP1A1 polymorphisms are potential risk factors for digestive tract cancer. PMID: 27061602
  41. was observed that the variant genotypes of GSTM1, GSTT1, GSTP1 and CYP1A1 did not significantly increase the risk of cervical cancer (CC), however, statistically significant increased risk was observed for women who used wood for cooking and had GSTM1 (null) genotype. PMID: 28361858
  42. CYP1A1*2A was not associated with Colorectal Cancer(CRC)development (OR = 1.566; 95% confidence interval [CI] = 0.90-2.73; p = 0.12); C allele-carrying individuals who had smoked within the past 5 years had a significant risk of CRC (OR = 2.28; 95% CI = 1.07-4.86; p = 0.043). PMID: 27243113
  43. CYP1A1 polymorphisms were not associated with sporadic colorectal neoplasms. PMID: 28018104
  44. Allele frequency of the CYP1A1 3801T>C polymorphism does not correlate with semen production as determined by sperm counts or sperm function as determined by fertilization rates with ICSI. PMID: 27117375
  45. data indicate that CYP1A1 I462V allele confers the least carcinogen-associated genotoxicity, compared to CYP1A1; however, results vary depending on the chemical carcinogen and the genotoxic endpoint PMID: 28283091
  46. By sequencing Apc and Ctnnb1 genes, we found that most PhIP-induced small intestinal tumors in obese mice carried only a single heterozygous mutation in Apc . Our findings demonstrate that PhIP-induced small intestinal carcinogenesis in hCYP1A-db/db mice is promoted by obesity and involves Apc mutation and inactivation by DNA hypermethylation PMID: 27207656
  47. The rapid activation of the Ah receptor and CYP1A1 protein expression by higher chlorinated polychlorinated naphthalene point to their toxicity; however, it is not sufficient for potential carcinogenicity. PMID: 27501765
  48. The polymorphism of CYP1A1*4 is associated with esophageal squamous cell carcinoma risk. PMID: 26455829
  49. Our results showed that 3 single nucleotide polymorphisms of ACE rs4340, IL-6 rs1800795, and NOS3 rs1799983 were significantly associated with the risks of mycoplasma pneumoniae pneumonia (MPP), while no differences were observed in genotype frequencies of GSTM1 and CYP1A1. The combinations of ACE/NOS3/CYP1A1 and ACE /NOS3 contribute to the genetic susceptibility of MPP in Chinese children. PMID: 28403117
  50. the CYP1A1 (rs4646422) polymorphism might be involved in gastric carcinogenesis among the Japanese population. PMID: 27062139
  51. Report quinone-mediated induction of cytochrome P450 1A1 in HepG2 cells through increased interaction of aryl hydrocarbon receptor with aryl hydrocarbon receptor nuclear translocator. PMID: 27853106
  52. Polymorphism in the CYP1A1 gene is an important risk modifier for lung cancer. PMID: 27396354
  53. The CYP1A1 Gene of rs4646903 minor alleles and interaction between rs4646903 and rs1048943 were associated with increased cervical cancer risk. PMID: 27265845
  54. RvD1 down-regulates cytochrome P450 (CYP1A1) and prostaglandin synthase 2 (PTGS2) gene expression. PMID: 27362771
  55. CYP1A1 gene polymorphisms and psychological distress act independently but do not interact with each other in pathogenesis of male infertility. PMID: 27220890
  56. This meta-analysis suggested that CYP1A1 Ile462Val polymorphism was associated with an increased risk of endometriosis, particularly in Asians. CYP1A1 MspI polymorphism may not be associated with endometriosis risk, but GSTM1 and CYP1A1 MspI polymorphism may have a joint effect on endometriosis risk PMID: 27525656
  57. The analysis results showed that the following polymorphisms were correlated with susceptibility to lung cancer: rs4646903 in CYP1A1 (P < 0.001), rs1048943 in CYP1A1 (P < 0.001), rs1695 in GSTP1 (P < 0.05), rs13181 in ERCC2 (P < 0.001), and rs25487 in XRCC1 (P < 0.05); no such correlation existed in rs861539 in XRCC3 (P > 0.05). PMID: 27819744
  58. This study showed that the CYP1A MspI polymorphism is associated with endometriosis, and that this type of polymorphism is more frequently observed in patients with infertility and severe endometriosis. PMID: 27706624
  59. polymorphisms of CYP1A1 gene were not associated with uterine leiomyoma susceptibility, however, the combination of the TT/AG genotypes and TG haplotype could increase risk PMID: 27333216
  60. Positive results were found in 15 situations when genes were analyzed in groups of 3; the most significant result corresponded to polymorphisms of p53, ERb and GSTM1 seen in 20%; PROGINS, ERb and GSTM1 in 18%; and p53, ERb and PROGINS in 12% patients., while 31.25% patients showed GSTM1- PROGINS and GSTT1-CYP1A1 polymorphism PMID: 27323100
  61. No association of functional polymorphisms in the estrogen metabolism genes CYP1A1 with menstrual migraine. PMID: 28089731
  62. 'AA' genotype at 2453 position and 'GG' genotype at 2455 position of CYP1A1 were the risk genotypes for presbycusis. PMID: 27562082
  63. suggest that biological activity of DMU-212 in ovarian cancer cells is related to its metabolic activation to DMU-214 by CYP1A1. PMID: 28049037
  64. Resveratrol and all its derivatives reduced also SULT1E1 mRNA transcript level. The reduced expression of AhR, CYP1A1, and 1B1 was also found as a result of treatment with these compounds. PMID: 27854074
  65. Data suggest that two inhibitors from grapefruit juice (naringenin and 6',7'-dihydroxybergamottin) block oxidation of 7-ethoxyresorufin via competitive binding to the active site of both human and rat CYP1A1. PMID: 27444380
  66. Sensitivity analysis suggested that the overall results showed no significant change in three genetic models when any one study was removed, and publication bias was undetected by the Egger test. The CYP1A1 MspI polymorphism may be associated with oral cancer risk in Asian populations PMID: 27323067
  67. Mixed-ligand copper(II) complexes activate AhR and induce AhR-dependent CYP!a1/2 genes in human hepatocytes. PMID: 27180721
  68. Metabolism of amiodarone to desethylamiodarone by CYP1A1 or CYP3A4 plays an important role in the hepatocellular toxicity of amiodarone. PMID: 27113703
  69. Results of the present study might be important in understanding the distribution of CYP1A1 (m1) and CYP1A1 (m2) polymorphisms in Mazandaran province of Iran. PMID: 27453279
  70. In this study, we found a significant association between genotypes of variants in rs4646903 with decreased ischemic stroke risk. PMID: 27383824
  71. The aim of this work is to review the origin, regulation, and structural and functional characteristics of CYP1A1 letting us understand its role in the bioactivation of precarcinogen and the consequences of its modulation in other physiological processes, as well as guide us in the study of this important protein. PMID: 28105425
  72. the CYP1A1 mRNA levels increased with the greatest effect at 100 nM of FICZ. The AhR protein levels significantly decreased as the FICZ concentration increased. Additionally, the CYP1A1 protein levels were notably increased PMID: 26861731
  73. Gene polymorphism of CYP1A1 at NcoI site was observed to be significantly higher. PMID: 27509973
  74. A positive relationship was identified between CYP1A1 mRNA levels and the presence of chronic disease. PMID: 27509995
  75. Study shows that the risk of developing hepatocellular carcinoma in Egyptian population can be partly explained by genetic polymorphisms in GSTP1 gene. PMID: 27271262
  76. CYP1A1 rs1048943 and rs4646903 polymorphisms contribute to risk of laryngeal cancer. PMID: 26578427
  77. Resulted in transient CYP1A1 and CYP1A2 mRNA accumulations. PMID: 26953171
  78. The C/C genotype of CYP1A1 MspI polymorphism is associated with the development of cervical carcinoma. PMID: 26838255
  79. CYP1A1 genetic variation may be associated with breast cancer in Pakistani population. PMID: 26545608
  80. No statistically significant overall association was found between breast cancer risk and CYP1A1 M2 genotypes. PMID: 26838244
  81. CYP1A1 2454A > G polymorphism is associated with colorectal cancer risk. PMID: 26881514
  82. polymorphisms in CYP2E1 (PstI) and CYP1A1 (MspI) were not associated with sporadic colorectal cancer in the evaluated Brazilian population. PMID: 26782431
  83. A significant association between the frequency of the CYP1A1 polymorphism and the development of cervical cancer. PMID: 26782546
  84. CYP1A1 and GSTM1 gene polymorphisms affect familial aggregation of esophageal cancer among the Kazakh ethnic group in Xinjiang. PMID: 26782562
  85. Data show a combined role of cytochrome P 450 1A1 (CYP1A1), Glutathione -S -transferase M1 (GSTM1) and T1 (GSTT1) polymorphisms and smoking status can be attributed for the cause of lung cancer. PMID: 27090234
  86. The allelic variation frequency of the CYP1A1 gene at exon 7 (Ile 462 Val) in bone tumor patients was significantly higher than that in control. PMID: 26681006
  87. CYP1A1 expression in 3D spheroids of liver cancer cells is regulated by AhR and PXR. PMID: 26643992
  88. The CYP1A1 gene deletion is a lung cancer risk factor, and interacts with smoking in non-small cell lung cancer development. PMID: 26782595
  89. The result indicates that the risk of Head and Neck Cancer associated with tobacco and alcohol is modulated by CYP1A1 T3801C polymorphism. PMID: 26514474
  90. the association between CYP1A1 polymorphisms and chronic obstructive pulmonary disease risk, is reported. PMID: 26425562
  91. This study evaluated the expression of FGF7, AhR, and CYP1A1 in colorectal cancer cells and revealed a new mechanism by which KGF promotes cell proliferation through the AhR-cyclin D1 pathway in colon cancer cells. PMID: 26514676
  92. meta-analysis provides evidence that CYP1A1 MspI polymorphism may contribute to esophageal cancer development in the Chinese population PMID: 25886559
  93. The present meta-analysis suggests that CYP1A1 polymorphism is not associated with bladder cancer risk. PMID: 24935599
  94. Aryl hydrocarbon receptor (AhR)-siRNA knockdown in human esophageal cells supported the hypothesis of AhR-mediated CYP1A1 induction by alternariol (AOH). However, DNA damage was minor at CYP1A1-inducing AOH concentrations. PMID: 26474839
  95. CYP1A1 A4889G and T6235C polymorphisms alter the risk and clinical or tumor characteristics of breast cancer among a heterogeneous population from Southeastern Brazil. PMID: 26598080
  96. Genetic variations in CYP1A1 and CYP1B1 and the serum levels of 25(OH)D showed synergistic effect on blood pressure, especially in individuals currently in treatment for hypertension. PMID: 25304467
  97. Hypermethylation of CpG island of CYP1A1 promoter and polymorphism is associated with anti-tuberculosis drug-induced liver injury. PMID: 25798582
  98. The colon tumors in hCYP1A mice mimic human colorectal carcinogenesis. PMID: 25131582
  99. In conclusion, the 3801 TC polymorphism in the CYP1A1 gene may be related to increased risk of hepatocellular carcinoma in Asians PMID: 25730047
  100. These findings suggest the Gly45Asp substitution results in a structural disturbance of CYP1A1, reducing its holoenzyme formation and catalytic activity. PMID: 26367467

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Subcellular Location Endoplasmic reticulum membrane, Peripheral membrane protein, Microsome membrane, Peripheral membrane protein
Protein Families Cytochrome P450 family
Tissue Specificity Lung, lymphocytes and placenta.
Database Links

HGNC: 2595

OMIM: 108330

KEGG: hsa:1543

STRING: 9606.ENSP00000369050

UniGene: Hs.72912

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