Recombinant Human E3 ubiquitin-protein ligase NRDP1(RNF41)

Code CSB-EP880976HU
Size US$2466Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names RNF41
Uniprot No. Q9H4P4
Research Area Cell Biology
Alternative Names 4930511A05Rik; 4933415P08Rik; E3 ubiquitin protein ligase NRDP1; E3 ubiquitin-protein ligase NRDP1; Fetal Liver RING finger protein; FLRF; MGC45228; Neuregulin receptor degradation protein 1; NRDP1; RING finger protein 41; rnf41; RNF41_HUMAN; SBBI03
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-317aa
Target Protein Sequence MGYDVTRFQGDVDEDLICPICSGVLEEPVQAPHCEHAFCNACITQWFSQQQTCPVDRSVVTVAHLRPVPRIMRNMLSKLQIACDNAVFGCSAVVRLDNLMSHLSDCEHNPKRPVTCEQGCGLEMPKDELPNHNCIKHLRSVVQQQQTRIAELEKTSAEHKHQLAEQKRDIQLLKAYMRAIRSVNPNLQNLEETIEYNEILEWVNSLQPARVTRWGGMISTPDAVLQAVIKRSLVESGCPASIVNELIENAHERSWPQGLATLETRQMNRRYYENYVAKRIPGKQAVVVMACENQHMGDDMVQEPGLVMIFAHGVEEI
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 62.9kDa
Protein Length Full Length
Tag Info N-terminal GST-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Data

Function Acts as E3 ubiquitin-protein ligase and regulates the degradation of target proteins. Polyubiquitinates MYD88. Negatively regulates MYD88-dependent production of proinflammatory cytokines. Can promote TRIF-dependent production of type I interferon and inhibits infection with vesicular stomatitis virus (By similarity). Promotes also activation of TBK1 and IRF3. Involved in the ubiquitination of erythropoietin (EPO) and interleukin-3 (IL-3) receptors. Thus, through maintaining basal levels of cytokine receptors, RNF41 is involved in the control of hematopoietic progenitor cell differentiation into myeloerythroid lineages (By similarity). Contributes to the maintenance of steady-state ERBB3 levels by mediating its growth factor-independent degradation. Involved in the degradation of the inhibitor of apoptosis BIRC6 and thus is an important regulator of cell death by promoting apoptosis. Acts also as a PRKN modifier that accelerates its degradation, resulting in a reduction of PRKN activity, influencing the balance of intracellular redox state. The RNF41-PRKN pathway regulates autophagosome-lysosome fusion during late mitophagy. Mitophagy is a selective form of autophagy necessary for mitochondrial quality control
Gene References into Functions
  1. Data (including data from studies using knockout and transgenic mice/cells) suggest that CLEC16A, NRDP1, and USP8 form tripartite complex; CLEC16A-NRDP1-USP8 complex appears to rely on ubiquitin signals to promote mitophagy and maintain mitochondrial function necessary for beta-cell function. (CLEC16A = C-type lectin domain family 16 member A; NRDP1 = ubiquitin-protein ligase NRDP1; USP8 = ubiquitin specific peptidase 8) PMID: 29180353
  2. Over-expression of miR-497 promotes the proliferation of glioma cells U87 by targeting Nrdp1. PMID: 28871945
  3. Authors conclude that the planar cell polarity (PCP) pathway contributes significantly to the motility and hence the invasiveness of glioblastoma multiforme (GBM) cells, and that Nrdp1 acts as a negative regulator of PCP signaling by inhibiting Dvl through a novel polyubiquitination mechanism. PMID: 28481871
  4. Data suggest that PHLPP1 plays an important role in assembly of kinetochores by counteracting RNF41-mediated SGT1 degradation. (PHLPP1 = PH domain and leucine rich repeat protein phosphatase 1; RNF41 = ring finger protein 41; SGT1 = suppressor of G2 allele of SKP1) PMID: 28696259
  5. Results show low expression level of NRDP1 in colorectal neoplasm independently of tumor stage. PMID: 27648936
  6. findings suggest that HBx promotes the progression of hepatocellular carcinoma by decreasing the stability of Nrdp1, which results in up-regulation of ErbB3 PMID: 26846102
  7. the association between RNF41 polymorphisms and congenital heart disease in the Chinese Mongolian population PMID: 27323192
  8. Nrdp1S is a tumour suppressor that which potentiates the Nrdp1-mediated ubiquitination and degradation of ErbB3. An Nrdp1S deficiency may also be an important factor in the loss of Nrdp1. PMID: 26612725
  9. HPV 16 E2 can modulate ErbB-3 by interacting with Nrdp-1, which is involved in the regulation of this receptor, via ubiquitination and degradation. PMID: 26963794
  10. The results of this study suggested that Nrdp1-mediated ErbB3 degradation suppresses glioma migration and invasion and that loss of Nrdp1 may amplify ErbB3 signaling to contribute to glioma migration and invasion. PMID: 26088461
  11. ABPN downregulates multiple components of the EGFR/ErbB3 signaling pathway through Nrdp1 activation. PMID: 26464195
  12. Nrdp1 may serve as a useful biomarker for the clinical outcome and efficacy of adjuvant anthracyclines-based chemotherapy in breast cancer. PMID: 25519010
  13. Lower expression of Nrdp1 in human glioma contributes tumor progression by reducing apoptosis. PMID: 25355637
  14. Phosphorylation of RNF41 by Par-1b regulates basolateral membrane targeting of laminin-111 receptors. PMID: 24259665
  15. Balanced reciprocal cross-regulation of RNF41 and USP8 determines whether receptors are sorted for lysosomal degradation or recycling, this way regulating basal cytokine receptor levels. PMID: 23750007
  16. Coiled-coil domain deletion abrogates Nrdp1 oligomerization and suppresses Nrdp1 but not ErbB3 ubiquitination and degradation. PMID: 24519943
  17. Data demonstrate that Nrdp1 preferentially associates with the nascent form of ErbB3 to accelerate its degradation, and that the two proteins colocalize at the endoplasmic reticulum. PMID: 21576364
  18. these data provide insights into understanding the potential role of Nrdp1 in cell growth, apoptosis and oxidative stress, and in the pathogenesis of Parkinson's disease. PMID: 21693106
  19. RNF41 interacts with JAK2-associated cytokine receptor complexes and modulates their cell surface exposure and signalling. PMID: 21378310
  20. Post-transcriptional mechanisms contribute to the suppression of the ErbB3 negative regulator protein Nrdp1 in mammary tumors. PMID: 20628057
  21. Observations point to a model of CRPC development in which progression of PCa to castration resistance is associated with the inability of AR to transcriptionally regulate Nrdp1, and predict that inhibition of ErbB3 during AW may impair CRPC development. PMID: 20587519
  22. This study do not support the hypothesis of a major role for the Nrdp1 gene in PD development in the Chinese population. PMID: 19800834
  23. RNF41 mRNA measured by real time reverse-transcriptase polymerase chain reaction reaction quantifies heart allograft apoptosis in graft rejection. PMID: 20098290
  24. Nrdp1 can be important in the initiation of apoptosis by catalyzing ubiquitination and degradation of BRUCE. PMID: 14765125
  25. Results indicate that Nrdp1 is a specific target for the USP8 deubiquitinating enzyme and are consistent with a model where USP8 augments Nrdp1 activity by mediating its stabilization PMID: 15314180
  26. The USP8 recognition domain of NRDP1 has a novel protein fold that interacts with a conserved peptide loop of the rhodanese domain. PMID: 17035239
  27. Neuregulin 1-induced protein stability cascade involving USP8 and Nrdp1 mediates the down-regulation of ErbB3 PMID: 17210635

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Tissue Specificity Detected in ovary, testis and prostate.
Database Links

HGNC: 18401

KEGG: hsa:10193

STRING: 9606.ENSP00000342755

UniGene: Hs.524502

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