Recombinant Human E3 ubiquitin-protein ligase TRIM50 (TRIM50)

Code CSB-YP768234HU
MSDS
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Source Yeast
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Code CSB-EP768234HU
MSDS
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Source E.coli
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Code CSB-EP768234HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP768234HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP768234HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TRIM50
Uniprot No.
Alternative Names
E3 ubiquitin ligase ; E3 ubiquitin protein ligase TRIM50; E3 ubiquitin-protein ligase TRIM50; TRI50_HUMAN; TRIM50; TRIM50A ; Tripartite motif containing 50; Tripartite motif containing protein 50; Tripartite motif protein 50; tripartite motif protein 50A ; tripartite motif-containing 50A ; Tripartite motif-containing protein 50
Species
Homo sapiens (Human)
Expression Region
1-487
Target Protein Sequence
MAWQVSLLEL EDWLQCPICL EVFKEPLMLQ CGHSYCKGCL VSLSCHLDAE LRCPVCRQAV DGSSSLPNVS LARVIEALRL PGDPEPKVCV HHRNPLSLFC EKDQELICGL CGLLGSHQHH PVTPVSTVYS RMKEELAALI SELKQEQKKV DELIAKLVNN RTRIVNESDV FSWVIRREFQ ELHHLVDEEK ARCLEGIGGH TRGLVASLDM QLEQAQGTRE RLAQAECVLE QFGNEDHHKF IRKFHSMASR AEMPQARPLE GAFSPISFKP GLHQADIKLT VWKRLFRKVL PAPEPLKLDP ATAHPLLELS KGNTVVQCGL LAQRRASQPE RFDYSTCVLA SRGFSCGRHY WEVVVGSKSD WRLGVIKGTA SRKGKLNRSP EHGVWLIGLK EGRVYEAFAC PRVPLPVAGH PHRIGLYLHY EQGELTFFDA DRPDDLRPLY TFQADFQGKL YPILDTCWHE RGSNSLPMVL PPPSGPGPLS PEQPTKL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
E3 ubiquitin-protein ligase that ubiquitinates Beclin-1/BECN1 in a 'Lys-63'-dependent manner enhancing its binding to ULK1. In turn, promotes starvation-induced autophagy activation. Interacts also with p62/SQSTM1 protein and thereby induces the formation and the autophagy clearance of aggresome-associated polyubiquitinated proteins through HDAC6 interaction.
Gene References into Functions
  1. TRIM50, via the RING domain, ubiquitinates Beclin 1 in a K63-dependent manner enhancing its binding with ULK1 and autophagy activity. PMID: 29604308
  2. TRIM50 K372 residue competes for post-translational protein modifications such as acetylation and ubiquitination. PMID: 24308962
  3. When the proteasome activity is impaired, TRIM50 fails to drive its substrates to the proteasome-mediated degradation. PMID: 22792322
  4. Encodes an E3 ubiquitin ligase; putatively involved in phenotype determination of Williams-Beuren syndrome. PMID: 18398435
Subcellular Location
Cytoplasm.
Protein Families
TRIM/RBCC family
Database Links

HGNC: 19017

OMIM: 612548

KEGG: hsa:135892

STRING: 9606.ENSP00000327994

UniGene: Hs.647053

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