Recombinant Human Eukaryotic translation initiation factor 4 gamma 2(EIF4G2) ,partial

Code CSB-YP007569HU
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Source Yeast
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Code CSB-EP007569HU
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Source E.coli
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Code CSB-EP007569HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007569HU
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Source Baculovirus
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Code CSB-MP007569HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names EIF4G2
Uniprot No. P78344
Alternative Names DAP-5; DAP5; Death associated protein 5; Death-associated protein 5; eIF-4-gamma 2; eIF-4G 2; eIF4G 2; EIF4G2; Eukaryotic translation initiation factor 4 gamma 2; IF4G2_HUMAN; Nat1; Novel APOBEC-1 target 1; p97; Translation repressor NAT1 ; Translation repressor NAT1
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.
Gene References into Functions
  1. The percentage at the S-stage was decreased in the miR-379 mimics and EIF4G2 shRNA groups. PMID: 28381518
  2. DAP5 knockdown from human ESCs (hESCs) resulted in persistence of pluripotent gene expression, delayed induction of differentiation-associated genes in different cell lineages, and defective embryoid body formation PMID: 27664238
  3. Data show that microRNA miR-379 potentiated lung cancer (LCa) chemosensitivity via modulation of cisplatin (CDDP)-induced apoptosis by directly targeting the eukaryotic translation initiation factor 4 gamma 2 (EIF4G2) 3' UTR. PMID: 28117895
  4. These results indicate that miR-139 is capable of inhibiting chondrocyte proliferation and migration, thus being a possible therapeutic target for OA. The mechanism of miR-139 in chondrocytes may be related to its regulation on EIF4G2 and IGF1R. PMID: 27105918
  5. The Coxsackievirus B3 protease 2A-mediated cleavage of DAP5 results in the production of two truncates that exert differential effects on protein translation of the IRES-containing genes, leading to enhanced host cell death. PMID: 26586572
  6. Knockdown of EIF4G2 recapitulated the effects of mir-139, whereas restoring EIF4G2 expression rescued the mir-139 phenotype. elevated miR-139-5p expression is associated with a favorable outcome in acute myeloid leukemia. PMID: 26165837
  7. findings provide the first mechanistic insights into the function of DAP5 as a selective regulator of cap-independent translation PMID: 25779044
  8. Our results provide evidence that the tumor suppressor effect of miR-520c-3p is mediated through repression of translation while inducing senescence and that eIF4GII is a key effector of this anti-tumor activity. PMID: 24497838
  9. DAP5, a translation initiation factor shown to positively regulate the translation of various internal ribosome entry sites containing mRNAs, promotes internal ribosome entry site-driven translation of p53 mRNA. PMID: 23318444
  10. DAP5/p97 and DAP5/p86 enhanced the translation of the anti-apoptotic protein Bcl-2 and inhibited cisplatin-induced apoptosis PMID: 22555068
  11. Multiple isoforms of eIF4GII arise from multiple promoters & alternative splicing events. A non-canonical CUG initiation codon extends the eIF4GII N-terminus. The eIF4GII N-terminus plays an alternative role in initiation factor assembly. PMID: 22909319
  12. crystal structure of C-terminal region (residues 540-897) of p97/EIF4G2 (at 2.0 Angstrom resolution): sequence/structure homology with EIF4G1 PMID: 20544972
  13. crystallization and preliminary X-ray diffraction analysis of the MIF4G domain of DAP5 is presented. PMID: 20057060
  14. Includes the functional analysis of a similar protein in yeast. PMID: 11331597
  15. The caspase-cleaved DAP5 protein supports internal ribosome entry site-mediated translation of death proteins PMID: 11943866
  16. endogenous eIF4GII was phosphorylated by Ca(2+)/calmodulin-dependent protein kinase I PMID: 14507913
  17. Cleavage of eIF4GII is induced in cells and in cell extracts by the FMDV leader protease (L(pro)) alone PMID: 15016848
  18. EIF4G2 transcripts exhibited three different expression patterns, downregulation was found in about 50% of the cases analyzed and EIF4G2 gene transcription was associated with invasive tumors. PMID: 16410684
  19. p97 is functionally different from the closely related C-terminal two-thirds of eIF4GI and it can positively promote protein synthesis and cell proliferation. PMID: 16932749
  20. These data indicate that full-length p97 is a translational activator with essential role(s) in unstressed cells, suggesting a reassessment of current models of p97 function. PMID: 17237356
  21. Data show that DAP5 promotes cap-independent translation of Bcl-2 and CDK1 to facilitate cell survival during mitosis. PMID: 18450493
  22. role and regulation of death-associated protein-5 (DAP5/p97/NAT1), a novel inhibitor of translational initiation, in acute promyelocytic leukemia cell differentiation and apoptosis PMID: 18491231
  23. Results report the crystal structure of the C-terminal region of DAP5/p97 extending between amino acids 730 and 897. PMID: 18722383
  24. Exclusive use of a GUG translation initiation codon is conserved in human, chicken, Xenopus, and zebrafish. PMID: 15718103
  25. translation starts exclusively at a non-AUG translation initiation codon (GUG). PMID: 9049310

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Protein Families Eukaryotic initiation factor 4G family
Tissue Specificity Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney.
Database Links

HGNC: 3297

OMIM: 602325

KEGG: hsa:1982

STRING: 9606.ENSP00000340281

UniGene: Hs.183684

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