Recombinant Human Homeobox protein Nkx-2.1 (NKX2-1)

1. We observed that hyaline membrane disease is the most common cause of perinatal death among respiratory disorders, and in this disease, the expression of TTF-1 is significantly reduced in terminal bronchioles, distal airways, and alveoli compared to the control group. PMID: 30004050
2. NKX2-1 expression was upregulated in never-smokers , lung adenocarcinoma and patients with wild-type TP53. A negative correlation between NKX2-1 and miR-365 expression was found but there was no correlation between NKX2-1 and miR-33a expression. NKX2-1 expression impacts prognosis in early-stage non-small cell lung cancer patients, particularly in those with neither TP53 nor KRAS mutations. PMID: 29237428
3. Study shows that that NKX2-1 directly and positively regulates transcription of cyclin D1 in lung adenocarcinoma. Also, the expression of NKX2-1, but not cyclin D1, is significantly associated with metastatic incidence as an independent good prognostic factor of adenocarcinoma. PMID: 28634225
4. Case Report: TTF-1 expression in of a soft tissue metastasis of mediastinal non-functioning paraganglioma. PMID: 29517207
5. Loss of NKX2-1 is associated with cancer. PMID: 29587142
6. TTF-1 might be involved in lymph node metastasis of ovarian carcinomas in the presence of lymphangiogenesis PMID: 28843751
7. results indicate that TTF-1 expression in NECs suggests a possible role in their normal development and differentiation PMID: 25722034
8. Combined genomic and proteomic analyses demonstrated infrequent alteration of validated lung cancer targets but identified novel potential targets for TTF1-negative LUAD, including KEAP1/Nrf2 and DNA repair pathways PMID: 25878335
9. Low expression levels of TTF-1 is associated with lung adenocarcinoma. PMID: 25982999
10. Immunohistochemical comparison of two TTF-1 mAbs in atypical squamous lesions and sarcomatoid carcinoma of the lung, and pleural malignant mesothelioma suggest possibility for diagnostic errors. PMID: 26281863
11. Because TTF-1 is not detected in the vast majority of cases using a separate antibody clone, 8G7G3/1, we conclude that aberrant staining is due to cross-reactivity to unknown antigen(s). TTF-1 positivity and even Napsin A positivity, therefore, cannot be used as conclusive evidence of pulmonary origin and gastrointestinal origin must be considered in the differential diagnosis PMID: 26469324
12. CK7, TTF-1 and napsin A are predominantly expressed in primary lung adenocarcinoma patients, with CDX-2 being inconsistently expressed. PMID: 26469326
13. Study showed that TTF1 expression was upregulated in tumor tissues from patients with hepatocellular carcinoma (HCC) and that TTF1 mRNA levels were significantly associated with poor prognoses. Moreover, upregulation of TTF1 was shown to promote the proliferation of HCC cells in vitro. PMID: 26821084
14. P-AKT and TTF-1 using IHC had statistically significant correlation with EGFR mutation with high negative predictive value PMID: 26905105
15. TTF-1 positivity does not exclude the diagnosis of primary Olfactory neuroblastoma, although usually only a small percentage of cells are positive. PMID: 27543867
16. TTF-1 immunoreactivity was seen in 19 pulmonary neuroendocrine carcinoma (PNEC) cases (95%) and 13 thymic (TNEC) cases (59.1%). TTF-1 positivity was associated with high sensitivity but low specificity for PNEC, and adding PAX8 negativity significantly increased the specificity. PAX8 positivity alone showed essentially 100% specificity and 86.4% sensitivity for TNEC. PMID: 27761900
17. Studied the prognostic relevance of thyroid transcription factor 1 (TTF1) copy number alterations (CNAs) and expression levels in non-small cell lung cancer; found TTF1 CNAs and expression high within tumors and between primary and metastatic tumors. PMID: 28378892
18. it may be useful to combine NAPA and TTF-1 for increased sensitivity in lung cancer diagnostics. There is no substantial difference between monoclonal and polyclonal p40 and between different NAPA clones, whereas there is a difference between the TTF-1 clones 8G7G3/1 and SPT24 PMID: 26447895
19. TTF-1 shows significant potential as a diagnostic marker to differentiate metastatic pulmonary from non-pulmonary adenocarcinomas in pleural or other effusions. PMID: 26806377
20. Thyroid Transcription Factor 1 Alters the Expression Profiles of Cytokine and Angiogenic Factors of Lung Cancer Cells. PMID: 26912193
21. TTF1-expression had no significant impact on outcomes after irradiation of limited-disease small cell lung cancer. PMID: 27354614
22. our findings show that miR-532-5p is a novel transcriptional target of TTF-1 that plays a tumor suppressive role by targeting KRAS and MKL2 in lung adenocarcinoma PMID: 28474808
23. The genetic or epigenetic inactivation of NKX2-1/TTF-1 may play an essential role in the development and aberrant differentiation of non-TRU-type lung adenocarcinomas. PMID: 28677170
24. OLIG2 immunoreactivity was observed in GABAergic cells of the proliferative zones of the MGE and septum, but not necessarily co-expressed with NKX2.1, and OLIG2 expression was also extensively seen in the LGE, CGE, and cortex PMID: 27905023
25. the downregulation of NKX2-1 in IL-5+ NPs may be associated with tissue eosinophilia and goblet cells hyperplasia PMID: 28318118
26. In conclusion, TTF-1 is a useful marker in distinguishing subependymal giant cell astrocytoma from its mimics. Expression of TTF-1 in the fetal medial ganglionic eminence indicates that subependymal giant cell astrocytoma may originate from the progenitor cells in this region. PMID: 27910945
27. Low NKX2-1 expression is associated with thyroid carcinomas. PMID: 27036019
28. One familial cohort reported that Neuroendocrine cell hyperplasia of infancy (NEHI) is associated with a heterozygous variant of NKX2.1/TTF1. Four adult relatives with heterozygous NKX2.1 mutation and with clinical histories compatible with NEHI enrolled. Although clinical improvement occurs over time, NEHI may result in lifelong pulmonary abnormalities in some cases. PMID: 27187870
29. Patient-related factors modify the predisposition to papillary thyroid carcinoma by increasing the risk for rs944289 (near the NKX2-1 locus) per year of age, and by enhancing the protective effect of the FOXE1 GGT haplotype in men. PMID: 28660995
30. The homeobox domain-containing transcription factor NKX2.1 is highly expressed in the medial ganglionic eminence (MGE) and pre-optic area of the ventral subpallium and is essential for specifying cortical interneuron fate. PMID: 27539622
31. We demonstrated that the 14q13.2q21.1 deletion, which encompasses NKX2-1, but not FOXG1 gene and HPE8 region, identifies a well defined, more benign, microdeletion syndrome PMID: 27148860
32. Findings suggest that the novel non-transcriptional function of TTF-1 identified in this study may contribute to lung adenocarcinoma development by conferring tolerance to DNA RS, which is known to be inherently elicited by activation of various oncogenes. PMID: 28192407
33. Immunohistochemical detection of thyroid transcription factor 1, Napsin A, and P40 fragment of TP63 can be used in the subclassification of non-small cell lung carcinomas. PMID: 27045515
34. The rate of TTF-1-positive circulating tumor cells was strongly correlated with TNM staging, vascular infiltration, lymphatic metastasis, and the levels of CA125, CA15.3, and HE4 in endometrial carcinoma. PMID: 28039713
35. Study postulated that both TTF-1 and PAX-8 when co-expressed and have anti-proliferative and anti-tumorigenic properties up to a threshold expression level and beyond that, are able to induce pro-tumorigenic effects in thyroid carcinomas. PMID: 27573549
36. Results suggest that the thyroid transcription factor 1 expression was independently associated with progression-free survival and overall survival in patients with advanced-stage non-squamous non-small cell lung cancer treated with pemetrexed-based chemotherapy. PMID: 28218046
37. These findings describe recurrent NKX2-1 mutations in invasive mucinous adenocarcinomas of the lung and support NKX2-1 as a lineage-specific tumor suppressor gene in lung carcinogenesis. PMID: 26829311
38. Report TTF1 expression is common in combined Merkel cell carcinoma. PMID: 27322785
39. Preoperative serum miR-365 and TTF-1 mRNA levels may be effective indicators of tumor aggressiveness in human NSCLC. miR-365 and its target gene TTF-1 appear to be synergistic risk factors for the reduction in overall survival of patients with NSCLC. PMID: 26337230
40. In adenocarcinomas from extrahepatic biliary tract, there was no correlation between TTF-1 expression and the clinicopathological characteristics. PMID: 26316052
41. Suggest TTF-1 is a reliable marker in non-small cell lung carcinoma and can be used in differential diagnosis. PMID: 26456962
42. genetic susceptibility to thyroid cancer seems likely to be associated with the risk allele at rs944289 PMID: 26206751
43. EGFR knockdown led to upregulation of NKX2-1, suggesting a negative feedback loop. combined knockdown of NKX2-1 and EGFR in NCI-H1819 lung cancer cells reduced cell proliferation more than knockdown of either alone. PMID: 26556242
44. TTF-1 had no prognostic value concerning OS, but may serve as a predictor for response to first line chemotherapy in small cell lung cancer. PMID: 25889870
45. Authors suggest that NKX2-1 as a tumour suppressor or a tumour promoter in lung adenocarcinoma progression is dependent on p53 status. PMID: 25881545
46. TTF-1 is considered to be an excellent marker of pituicytes, specialized glia of the neurohypophysis. PMID: 25893822
47. Aberrant expression of mir-365/TTF-1 may be involved in the tumor development in patients with non-small cell lung carcinoma. PMID: 26045746
48. Different staining patterns can be seen with CK5/6 and p63; however, if they are used together with TTF-1 they can be used in subtyping lung neoplasms. PMID: 25944390
49. No TTF1 or EAP1 germline mutations were associated with central pubertal disorders. TTF1 and EAP1 may affect puberty by changing expression in response to other members of puberty-associated gene networks PMID: 24051510
50. Loss of TTF-1 is associated with low response to chemotherapy in non-small-cell lung cancer. PMID: 24457236

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HGNC: 11825

OMIM: 118700

KEGG: hsa:7080

STRING: 9606.ENSP00000346879

UniGene: Hs.94367