Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Neuroscience
Alternative Names
Gonadotropin receptor; Gpcr19-rs1; GTHR-II; HHG; LCGR; LGR2; LH-R; LH/CG R; LH/CG-R; LH/CGR; LHCGR; LHR; LHRHR; LSH R; LSH-R; LSHR_HUMAN; Luteinizing hormone receptor; Luteinizing hormone/choriogonadotropin receptor; Lutropin choriogonadotropic hormone receptor ; Lutropin choriogonadotropic receptor ; Lutropin-choriogonadotropic hormone receptor; ULG5
Species
Homo sapiens (Human)
Expression Region
27-363aa
Target Protein Sequence
EALCPEPCNCVPDGALRCPGPTAGLTRLSLAYLPVKVIPSQAFRGLNEVIKIEISQIDSLERIEANAFDNLLNLSEILIQNTKNLRYIEPGAFINLPRLKYLSICNTGIRKFPDVTKVFSSESNFILEICDNLHITTIPGNAFQGMNNESVTLKLYGNGFEEVQSHAFNGTTLTSLELKENVHLEKMHNGAFRGATGPKTLDISSTKLQALPSYGLESIQRLIATSSYSLKKLPSRETFVNLLEATLTYPSHCCAFRNLPTKEQNFSHSISENFSKQCESTVRKVNNKTLYSSMLAESELSGWDYEYGFCLPKTPRCAPEPDAFNPCEDIMGYDFLR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Extracellular Domain
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
This recombinant Human LHCGR protein is typically achieved by the manipulation of LHCGR gene expression in E.coli cells so that it expresses large amounts of a recombinant LHCGR gene. In order to get enough amount of the LHCGR protein, strain selection, codon optimization, fusion systems, co-expression, mutagenesis, and isotope labeling techniques are used. Finally, the LHCGR protein is isolated from the samples such as cell lysates or medium. Protein refolding, cleavage of fusion moieties and chromatography techniques are involved in the protein purification process.
The human LHCGR is a G-protein coupled receptor activated by both human chorionic gonadotropin (hCG) and luteinizing hormone (LH) to function in sexual differentiation in males, ovarian development in females. LHCGR is primarily expressed in ovarian tissues, where it triggers functional responses via cyclic adenosine mononucleotide (cAMP), calcium, and extracellular signal-regulated kinase (ERK) signaling. Appropriate levels of expression of LHCGR are crucial for luteinizing hormone (LH) to regulate ovarian function, including preovulatory steroidogenesis, ovulation, corpus luteum formation, and luteal cell steroidogenesis. Inactive variants of the LHCGR gene lead to Leydig cell hypoplasia (LCH), rare disease and one of the causes of disorder of sexual differentiation (DSD) in males.
