Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

LHCGR

Uniprot No.

P22888

Research Area

Neuroscience

Alternative Names

Gonadotropin receptor; Gpcr19-rs1; GTHR-II; HHG; LCGR; LGR2; LH-R; LH/CG R; LH/CG-R; LH/CGR; LHCGR; LHR; LHRHR; LSH R; LSH-R; LSHR_HUMAN; Luteinizing hormone receptor; Luteinizing hormone/choriogonadotropin receptor; Lutropin choriogonadotropic hormone receptor ; Lutropin choriogonadotropic receptor ; Lutropin-choriogonadotropic hormone receptor; ULG5

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

27-363aa

Target Protein Sequence

EALCPEPCNCVPDGALRCPGPTAGLTRLSLAYLPVKVIPSQAFRGLNEVIKIEISQIDSLERIEANAFDNLLNLSEILIQNTKNLRYIEPGAFINLPRLKYLSICNTGIRKFPDVTKVFSSESNFILEICDNLHITTIPGNAFQGMNNESVTLKLYGNGFEEVQSHAFNGTTLTSLELKENVHLEKMHNGAFRGATGPKTLDISSTKLQALPSYGLESIQRLIATSSYSLKKLPSRETFVNLLEATLTYPSHCCAFRNLPTKEQNFSHSISENFSKQCESTVRKVNNKTLYSSMLAESELSGWDYEYGFCLPKTPRCAPEPDAFNPCEDIMGYDFLR
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

41.6kDa

Protein Length

Extracellular Domain

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This recombinant Human LHCGR protein is typically achieved by the manipulation of LHCGR gene expression in E.coli cells so that it expresses large amounts of a recombinant LHCGR gene. In order to get enough amount of the LHCGR protein, strain selection, codon optimization, fusion systems, co-expression, mutagenesis, and isotope labeling techniques are used. Finally, the LHCGR protein is isolated from the samples such as cell lysates or medium. Protein refolding, cleavage of fusion moieties and chromatography techniques are involved in the protein purification process.

The human LHCGR is a G-protein coupled receptor activated by both human chorionic gonadotropin (hCG) and luteinizing hormone (LH) to function in sexual differentiation in males, ovarian development in females. LHCGR is primarily expressed in ovarian tissues, where it triggers functional responses via cyclic adenosine mononucleotide (cAMP), calcium, and extracellular signal-regulated kinase (ERK) signaling. Appropriate levels of expression of LHCGR are crucial for luteinizing hormone (LH) to regulate ovarian function, including preovulatory steroidogenesis, ovulation, corpus luteum formation, and luteal cell steroidogenesis. Inactive variants of the LHCGR gene lead to Leydig cell hypoplasia (LCH), rare disease and one of the causes of disorder of sexual differentiation (DSD) in males.

Target Background

Function

Receptor for lutropin-choriogonadotropic hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.

Gene References into Functions

This study demonstrates that both gonadotrophin receptors9 FSH receptor (FSHR) and LH/choriogonadotrophin receptor (LHCGR) are expressed along the glandular epithelium of endometria and they mediate the effects of gonadotrophins on intracellular functions. PMID: 29974367
Genotyping analysis showed no association between luteinizing hormone receptor (LHCGR) rs68073206 and male infertility, however, this polymorphism was significantly associated with testosterone levels in normozoospermic subjects. PMID: 28367994
Data suggest that, during follicle maturation, levels of MIR122 determine levels of LHR mRNA expression in the ovary. (LHR = luteinizing hormone receptor; MIR122 = microRNA 122) [REVIEW] PMID: 29544643
genetic association/pharmacogenetic studies in population in India: Data suggest that need for (and dose required of) fertility agent recombinant LH in addition to fertility agent recombinant FSH is associated with SNP in LHCGR in women undergoing ovarian stimulation in conjunction with in vitro fertilization. (LH = luteinizing hormone; FSH = follicle stimulating hormone) PMID: 29408742
hCG and its receptor, LH/CGR, are expressed in numerous sites of the reproductive tract, both in gonadal and extra-goanadal tissues, promoting oocyte maturation, fertilization, implantation and early embryo development PMID: 28505106
The reduced fertilisation and pregnancy rate was associated with a lower LH receptor density and a lack of essential down-regulation of the FSH and LH receptor. PMID: 28188844
This work demonstrates that the expression of FSHR and LHCGR can be induced in hGL5 cells but that the FSHR-dependent cAMP/PKA pathway is constitutively silenced, possibly to protect cells from FSHR-cAMP-PKA-induced apoptosis. PMID: 27502035
Inactivating mutations of the LHCGR gene may be a common cause of 46,XY primary amenorrhea. PMID: 27016457
data demonstrate that the majority of LHR mutations lead to intracellular retention and highlight the potential for novel pharmacological chaperone therapeutics that can "rescue" expression/function of retained mutant GPCRs. PMID: 27533885
expression and activation of LHCGR and ARF6 are up-regulated in GC from PCOS women but the mechanism of agonist-induced LHCGR internalization is unaltered PMID: 27061682
We showed that GNRHR and LHCGR were highly expressed in some wildtype aldosterone-producing adenoma samples, and that they positively correlated with GnRH-stimulated aldosterone production. PMID: 27196470
Mutation in the LHCGR gene is associated with Testotoxicosis. PMID: 26757946
The luteinizing hormone/human chorionic gonadotrophin receptor (LHCGR) variant N312S and the follicle-stimulating hormone receptor (FSHR) variant N680S can be utilized for the prediction of pregnancy chances in women undergoing IVF. PMID: 26769719
Data indicate two patients with peripheral precocious puberty and an activating mutation in the luteinizing hormone (LH))/choriogonadotropin receptor (LHCGR) gene. PMID: 26831561
The expression of miR-592 and its effects on LHCG receptors in patients with polycystic ovary syndrome and normal controls are described. PMID: 26679164
LHCGR levels in circulation predicts early and late preeclampsia in first trimester of pregnancy. PMID: 25676660
Data suggest that rs13405728 single nucleotide polymorphism of the luteinizing hormone receptor (LHR) gene may be associated with slow ovarian response in assisted reproductive technology (ART). PMID: 26663062
The insLQ polymorphism within LHR is associated with endometriosis and infertility. PMID: 25935136
the LHR can act in an invertebrate demonstrates the conservation of factors required for GPCR function among phylogenetically distant organisms. PMID: 26112185
Demonstrate the presence of LH receptors. Activation resulted in a dose-dependent increase in glucose-induced release of insulin. PMID: 25670721
Our aim was to study the association of LHCGR polymorphism (rs2293275) with PCOS PMID: 25565299
Melatonin affects the secretion of GnRH, LH and testosterone, improves sperm quality thereby regulating the testicular development and male reproduction. (Review) PMID: 25916694
This is the first study to confirm the association of novel LHCGR and FSHR SNPs with PCOS. The differential association of LHCGR and FSHR variants with PCOS confirms the racial/ethnic contribution to their association with PCOS. PMID: 25649397
Case Report: LHCGR nonsense mutation in an adult 46,XY female patient causing severe Leydig cell hypoplasia. PMID: 25079470
A high prevalence of endometriosis, polymorphism in the LHCGR and GnRH1 and progonadoliberin-2 antibodies in serum was found among the patients with severe dysmotility after treatment with GnRH analogs. PMID: 25592315
affected patients were all homozygous for a novel mutation in the LHCGR gene, a deletion of guanine in position 1850 (1850delG);the novel LHCGR mutation leads to complete inactivation of the LHCGR receptor due to trafficking and signaling abnormalities PMID: 25795638
Data indicate that all solid pseudopapillary tumor of the pancreas {SPT) presented membranous and cytoplasmic staining for luteinizing hormone receptor (LH receptor). PMID: 25010708
Data suggest LHR expression is up-regulated in granulosa cells and theca cells of early antral follicles, as follicles grow/mature, and in corpus luteum; low but significant expression of LHR is exhibited in cumulus cells (but not in stromal cells). PMID: 24874553
this is the first report suggesting that LHCGR genetic variation might function in patient risk for ovarian hyperstimulation syndrome PMID: 23883350
Silencing luteinizing hormone receptor expression suppresses androgen synthesis in prostate cancer cells. PMID: 25393079
genetic association study (case-control study) in population in Egypt: Data suggest that an SNP in LHCGR (rs2293275) is associated with polycystic ovary syndrome (PCOS); homozygous 935A women are at higher risk to develop PCOS. PMID: 24592983
miR-513a-3p is involved in the control of the LHCGR expression by an inversely regulated mechanism at the post-transcriptional level. PMID: 24747085
Data (including data from subjects with mutations) suggest that signaling via LHCGR by luteinizing hormone and chorionic gonadotropin plays physiological roles in human development and pathophysiological roles in reproductive disorders. [REVIEW] PMID: 24283620
during recovery from LH receptor desensitization, LH receptors are both located with DRM lipid environments and confined within small, mesoscale (80-160 nm) cell-surface compartments PMID: 23990106
Although luteinizing hormone and chorionic gonadotropin bind to a common receptor, emerging evidence suggests that they have disparate effects on downstream signaling cascades. (Review) PMID: 24365330
results of the experiments showed that hCGbeta action leads to the increase in ERK and AKT kinases phosphorylation in cancer cells and indicate that these biological effects can be achieved independently of human chorionic gonadotropin presence PMID: 24554412
LHCGR and 17alpha-hydroxylase/17-20-lyase (CYP17A1) protein levels are increased in polycystic ovaries (PCOs). PMID: 24014605
Regarding LHR, three patients were heterozygous for the known variant Glu354Lys and two for Ile374Thr. Intronic polymorphisms were not identified PMID: 23884663
hypomethylation of LHCGR is a potential mechanism underlying susceptibility to polycystic ovary syndrome PMID: 24527662
Examined the mechanisms of receptor signaling, intermolecular cooperation and intramolecular cis-activation. LHR-delExon10 was combined with LHR Lys605-->Glu, in which signaling is abolished, and the LHR mutant Cys131-->Arg, in which binding is deficient. PMID: 24438591
AdipoR2 is regulated by the LH receptor function via a cAMP dependent mechanism. PMID: 23779096
High expression/ activation of GPER, and low expression of LHCGR/FSHR correlate with significantly greatly survival in ovarian epithelial cancer patients. PMID: 23951246
Expression levels of LHR-associated protein are determined in granulosa-luteal cells by real-time reverse-transcription (PCR). PMID: 23433069
Data suggest that physical association of recombinant LHR (lutropin receptor) and FSHR (follitropin receptor) into heterodimeric complexes exhibits a pattern of cross-attenuation of signaling by each receptor and its hormone. PMID: 23825122
A complex neuroendocrine circuit exists in the retina, involving hCG secreting cells, hCG targets and hCG-release controlling cells. PMID: 23285091
case-control study comprising 651 TC cases and 313 controls in a Norwegian population was conducted for investigation of polymorphisms in the LHR, SRD5A and AR genes and their possible association with TC PMID: 22882535
The LHCGR is able to differentiate the activity of luteinizing hormone and chorionic gonadotropin. PMID: 23071612
A novel point mutation of the LHCGR gene has been identified in a family affected with FMPP. The c.1703C>T mutant LHCGR was confirmed to be constitutively active, which has led to maturation and proliferation of Leydig cells. PMID: 23225038
Mutations in LHCGR are relatively rare causes of mild and severe forms of hypergonadotropic hypogonadism or disorders of sex development leading to infertility, both male and female. [REVIEW] PMID: 23044874
The gene products LHCGR and FSHR therefore are likely to be important in the etiology of PCOS, regardless of ethnicity. PMID: 23118426

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Involvement in disease

Familial male precocious puberty (FMPP); Luteinizing hormone resistance (LHR)

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 1 family, FSH/LSH/TSH subfamily

Tissue Specificity

Gonadal and thyroid cells.

Database Links

HGNC: 6585

OMIM: 152790

KEGG: hsa:3973

STRING: 9606.ENSP00000294954

UniGene: Hs.468490

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Code	CSB-EP012911HU
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Lutropin-choriogonadotropic hormone receptor (LHCGR), partial

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