Recombinant Human Mucin-3A (MUC3A), partial

Code CSB-YP015225HU
MSDS
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Source Yeast
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Code CSB-EP015225HU
MSDS
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Source E.coli
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Code CSB-EP015225HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015225HU
MSDS
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Source Baculovirus
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Code CSB-MP015225HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MUC3A
Uniprot No.
Alternative Names
MUC3A; MUC3; Mucin-3A; MUC-3A; Intestinal mucin-3A
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Major glycoprotein component of a variety of mucus gels. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. May be involved in ligand binding and intracellular signaling.
Gene References into Functions
  1. MUC3A may have a role in poor prognosis in localized clear-cell renal cell carcinoma PMID: 27374181
  2. DNA hypomethylation in the 5'-flanking region of the MUC3A gene plays an important role in MUC3A expression in carcinomas of various organs. PMID: 20510874
  3. has highly conserved amino and carboxyl termini, suggesting a recent duplication of the entire ancestral gene PMID: 12958310
  4. MUC3 is overexpressed in renal clear cell carcinoma, and the MUC3 expression ratio is greater in nuclear grade 3 than in grades 1 and 2 (low grades) tumor. These findings suggest the implication of MUC3 in renal carcinogenesis. PMID: 14550470
  5. up-regulated MUC expression by synovial tissue cells and suggest a novel role of MUC3 and MUC5AC in the pathogenesis of arthritis PMID: 18163520
  6. We report the colonic adhesion mechanism of Lactobaillus plantarums LA318 is in part due to GAPDH binding to human ABO-type blood group antigens expressed on human colonic mucin (HCM). [HCM] PMID: 18790050
  7. Data show that the diagnostic sensitivity for mucin was 60%, and the specificity for mucin was 100%. PMID: 19365834
  8. Data show that special stains for mucin did not distinguish pseudocysts from neoplastic mucinous cysts. PMID: 19365837

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Subcellular Location
[Isoform 1]: Membrane; Single-pass membrane protein.; [Isoform 2]: Secreted.; [Isoform 3]: Secreted.; [Isoform 4]: Secreted.; [Isoform 5]: Secreted.
Tissue Specificity
Broad specificity; small intestine, colon, colonic tumors, heart, liver, thymus, prostate, pancreas and gall bladder.
Database Links

HGNC: 7513

OMIM: 158371

KEGG: hsa:4584

STRING: 9606.ENSP00000324834

UniGene: Hs.703577

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