Recombinant Human Multidrug resistance-associated protein 4(ABCC4) ,partial

Code CSB-YP001063HU
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Source Yeast
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Code CSB-EP001063HU
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Source E.coli
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Code CSB-EP001063HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001063HU
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Source Baculovirus
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Code CSB-MP001063HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ABCC4
Uniprot No. O15439
Alternative Names ABCC 4; ABCC4; ATP binding cassette sub family C (CFTR/MRP) member 4; ATP binding cassette sub family C member 4; ATP-binding cassette sub-family C member 4; bA464I2.1 (ATP binding cassette; sub-family C (CFTR/MRP) member 4); bA464I2.1; Canalicular multispecific organic anion transporter ; Canalicular multispecific organic anion transporter ABC superfamily; EST170205; MOAT B; MOAT-B; MOATB; MRP 4; MRP/cMOAT related ABC transporter; MRP/cMOAT-related ABC transporter; MRP4_HUMAN; Multi specific organic anion transporter B; Multi-specific organic anion transporter B; Multidrug resistance associated protein 4; Multidrug resistance-associated protein 4; OTTHUMP00000018560
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds and xenobiotics from cells. Transports a range of endogenous molecules that have a key role in cellular communication and signaling, including cyclic nucleotides such as cyclic AMP (cAMP) and cyclic GMP (cGMP), bile acids, steroid conjugates, urate, and prostaglandins. Mediates the ATP-dependent efflux of glutathione conjugates such as leukotriene C4 (LTC4) and leukotriene B4 (LTB4) too. The presence of GSH is necessary for the ATP-dependent transport of LTB4, whereas GSH is not required for the transport of LTC4. Mediates the cotransport of bile acids with reduced glutathione (GSH). Transports a wide range of drugs and their metabolites, including anticancer, antiviral and antibiotics molecules. Confers resistance to anticancer agents such as methotrexate.
Gene References into Functions
  1. Data show that forkhead box protein M1 (FoxM1) enhanced carboplatin resistance in Y-79CR cells through directly up-regulating the transcription of ATP-binding cassette transporter C4 (ABCC4). PMID: 30060118
  2. MRP4 is mainly responsible for the efflux of lamivudine and entecavir in hepatocyte cultures. PMID: 29568871
  3. Data suggest that targeting the ATP binding cassette subfamily C member 4 (ABCC4)-membrane palmitoylated protein 1 (MPP1) protein complex can lead to new therapies to improve treatment outcome of acute myeloid leukemia (AML). PMID: 29146910
  4. A functional polymorphism in the ABCC4 promoter, -1508A>G, may increase extracellular 15-hydroxyeicosatetraenoic acid, sphingosine-1-phosphate, and periostin levels, contributing to airway inflammation in asthmatics. PMID: 28659663
  5. MRP4 localisation and function are regulated by interactions with SNX27, PSD95, and AP3B1. PMID: 27761583
  6. MRP4 exports prostaglandin E2 PGE2 and contributes to cell migration/metastasis in basal/triple negative breast cancer cells. PMID: 28029661
  7. This study provides direct evidence that ganciclovir (GCV) is a substrate of the efflux transporter MRP4 and suggests that the intracellular fate of GCV is significantly influenced by ABCC4 rs11568658 variant in renal transplant recipients receiving GCV PMID: 27402191
  8. Multidrug resistance protein 4 (MRP4) is a critical protein associated with the antiviral efficacy of nucleos(t)ide analogue (NA)s, and combination therapy of NA and MRP inhibitors could reduce the dosage for long-term NA use. MRP4 expression is an important factor predicting treatment failure in chronic hepatitis B patients and will provide a potential therapeutic target against hepatitis B virus. PMID: 26931636
  9. All patients with both NUDT15 rs116855232 heterozygous variants and ABCC4 rs3765534 variants suffered from severe leukopenia and required 6-mercaptopurine dose reduction to less than 35 mg/m(2)/da PMID: 28883280
  10. ABCC4 variant (rs4148500) was significantly associated with hyperuricemia and gout. The gout risk allele was associated with fractional excretion of uric acid in male individuals. PMID: 28371506
  11. ABCC4 functional SNP in the 3' splice acceptor site of exon 8 (G912T) is associated with unfavorable clinical outcome in children with acute lymphoblastic leukemia PMID: 28550450
  12. Data suggest that common SNPs in MRP4/ABCC4 (evaluated here via site-directed mutagenesis in recombinant proteins expressed in kidney cell lines) are responsible for variations in transport of methylated arsenic metabolites (major urinary arsenic metabolites in humans), prostaglandin E2, and estradiol-17 beta-glucuronide. PMID: 27659809
  13. Data indicate that R-flurbiprofen inhibits multidrug resistance-associated protein 4 (MRP4, ABCC4)-mediated prostaglandins (PG) transport. PMID: 28042832
  14. Analysis of gene-gene interactions with CYP2B6 was particularly useful to reinforce the role of MRP4 and to reveal unknown associations, such as that of DRD3. PMID: 27665700
  15. The tagSNP ABCC4 rs17268122 appears to be a prognostic factor in locally advanced rectal cancer treated with neoadjuvant chemoradiotherapy (nCRT) and surgery, independently of response to nCRT PMID: 28011504
  16. ABCC4 plays a protective role against cytarabine-mediated insults in leukemic and host myeloid cells. PMID: 26842729
  17. Results demonstrated the capacity of MRP4 to confer resistance to arsenite (AsIII), as evidenced by cell survival assays when treated with AsIII in the presence or absence of its two differential inhibitors. PMID: 26497925
  18. Osteoarthritis patient platelets presented a higher expression of MRP4. PMID: 26141932
  19. Sulfate metabolites R-6-S and R-4'-S were generated from raloxifene in SULT293 expression HEK293 cells. Cellular excretion of the raloxifene sulfates was mainly mediated by BCRP and MRP4. PMID: 26611713
  20. the specific COX-2 inhibitor meloxicam can increase the intracellular accumulation of doxorubicin and enhance doxorubicin-induced cytotoxicity in A549 cancer cells by reducing the expression of MRP1 and MRP4. PMID: 26600514
  21. MRP4 overexpression is important to reduce aspirin-selective cell death PMID: 26491233
  22. Approximately 20% of HIV-infected patients receiving tenofovir showed beta2-microglobulinuria. The C allele at position 4976 of the ABCC4 gene was associated with beta2-microglobulinuria in this population. PMID: 26807589
  23. MRP4 genotyping may be useful for personalizing the therapeutic dose of 6-mercaptopurine during the acute lymphoblastic leukemia maintenance therapy in Japanese PMID: 25403995
  24. Genetic variability in ABCC4 may contribute to misoprostol-induced fever in pregnant women PMID: 26122863
  25. ABCC4 levels were significantly upregulated both in human NK/T-cell lymphoma YTS and SNK-6 cells. ABCC4 overexpression induced chemotherapy resistance to epirubicin and cisplatin in YTS cells, and inhibited apoptosis. PMID: 26499190
  26. These findings have important clinical implications because they directly highlight an important relationship between ABCC4 transporter function and phosphodiesterases in accounting for the cAMP-directed activity of antithrombotic agents. PMID: 26405223
  27. Data show the influence of ABCC2 and ABCC4 polymorphisms on tenofovir plasma concentrations in Thai HIV-infected patients PMID: 25801567
  28. cAMP regulates MRP4 promoter activity PMID: 25790437
  29. This study aimed to establish SULT1A3 stably transfected HEK293 cells, and to determine the contributions of BCRP and MRP transporters to excretion of chrysin and apigenin sulfates. PMID: 26291395
  30. The overexpression of MRP4 is related to multidrug resistance in osteosarcoma cells PMID: 25879330
  31. Multiple drug resistance-associated protein 4 (MRP4), prostaglandin transporter (PGT), and 15-hydroxyprostaglandin dehydrogenase (15-PGDH) as determinants of PGE2 levels in cancer. PMID: 25433169
  32. These results suggest that eicosapentaenoic acid-derived prostaglandin PGE3, PGF(3alpha), and thromboxane B3 are substrates of ABCC4 and ABCC4 partially contributes to the release of PGE3 and PGF(3alpha). PMID: 25275481
  33. Similar molecular features between MRP4 and BSEP inhibitors may partially explain why various drugs have affinity for both transporters. PMID: 25735837
  34. inhibition of ABCC4 gene expression can inhibit the proliferation of multidrug-resistant gastric cancer cells and can enhance gastric cancer cell sensitivity to chemotherapeutic drugs. PMID: 25572969
  35. Our result indicates that GSTP1rs1695 polymorphism was strongly associated with the response of chemotherapy, whereas ABCC4rs9561778 polymorphism was significantly related with chemotherapy-induced toxicities. PMID: 25677905
  36. The blockade of ABC transporter MRP4 could help to improve drug effectiveness, reduce tumour growth and prevent recurrence in glioblastoma multiforme. PMID: 25028266
  37. SNPs most associated with Chemotherapy-induced peripheral neuropathy were in glutathione peroxidase 7 (GPX7) gene. PMID: 25586538
  38. Influence of PTGS1, PTGFR, and MRP4 genetic variants on intraocular pressure response to latanoprost in Chinese primary open-angle glaucoma patients PMID: 25339146
  39. MRP4/ABCC4 has a relevant role in tumor growth and apoptosis and in the eradication of leukemic stem cells, providing the basis for a novel promising target in acute myeloid leukemia therapy. PMID: 25301721
  40. Upregulation of COX-2 in lung cancer promotes overexpression of MRP4 via PGE2-dependent pathway PMID: 24909729
  41. Hepatically derived enalaprilat is excreted across the hepatic basolateral membrane by MRP4. PMID: 24958844
  42. Multi-walled carbon nanotubes may act on c-Myc to down-regulate ABCB1/ABCC4 expression. PMID: 25461681
  43. role of MRP4 in thrombus formation PMID: 24836986
  44. Genetic variability in key genes in prostaglandin E2 pathway (COX-2, HPGD, ABCC4 and SLCO2A1) and their involvement in colorectal cancer development. PMID: 24694755
  45. study suggests that ABCC4 serves as a novel predictive biomarker that is responsible for the radioresistance and predicts a poor prognosis for locally advanced rectal carcinoma after neoadjuvant radiotherapy PMID: 24454870
  46. Knockdown of ABCC4 blocked the cell cycle progression in MGC80-3 gastric cancer cells. PMID: 24606721
  47. ABCC4 activates oncogenic pathways in esophageal squamous cell carcinoma and facilitates cancer cell development and progression. PMID: 24510239
  48. Human MRP4 could be a major player in the elimination of arsenic. PMID: 24870404
  49. NHERF3 is a key regulator of organic transport in the kidney, particularly MRP4-mediated clearance of drug molecules. PMID: 24436471
  50. These results suggest that in epithelial ovarian cancer, ABCC1/MRP1 may be a marker for aggressiveness because its expression was associated with tumor grade and support that ABCC4/MRP4 may play an unfavourable role in disease outcome. PMID: 24024181

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Subcellular Location Basolateral cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein.
Protein Families ABC transporter superfamily, ABCC family, Conjugate transporter (TC 3.A.1.208) subfamily
Tissue Specificity Widely expressed, with particularly high levels in prostate, but is barely detectable in liver. sinusoidal membrane of hepatocytes.
Database Links

HGNC: 55

OMIM: 605250

KEGG: hsa:10257

STRING: 9606.ENSP00000366084

UniGene: Hs.508423

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