Recombinant Human NLR family CARD domain-containing protein 4(NLRC4) ,partial

Code CSB-YP873615HU
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Source Yeast
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Code CSB-EP873615HU
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Source E.coli
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Code CSB-EP873615HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP873615HU
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Source Baculovirus
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Code CSB-MP873615HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names NLRC4
Uniprot No. Q9NPP4
Alternative Names and NACHT-containing protein; CARD 12; CARD; CARD LRR and NACHT containing protein 1; CARD LRR and NACHT containing protein; CARD12; Caspase recruitment domain containing protein 12; Caspase recruitment domain family member 12; Caspase recruitment domain-containing protein 12; CLAN 1; CLAN A; CLAN; CLAN B; CLAN C; CLAN D; Clan protein; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR 2.1; CLR2.1; ICE protease activating factor; Ice protease-activating factor; Ipaf; LRR; NLR family CARD domain containing 4; NLR family CARD domain-containing protein 4; NLRC 4; NLRC4; NLRC4_HUMAN; NOD like receptor C4; Nucleotide binding oligomerization domain leucine rich repeat and CARD domain containing 4; UNQ6189/PRO20215
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

(From Uniprot)
Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis.
Gene References into Functions
  1. Study findings suggest that NLRC4 may be involved in the exacerbation or modification of psoriatic lesions. PMID: 29797527
  2. Cryo-EM structures of ASC and NLRC4 CARD filaments reveal a unified mechanism of nucleation and activation of caspase-1 PMID: 30279182
  3. obesity-associated NLRC4 inflammasome activation/ interleukin-1 signalling promotes breast cancer progression in humans and in mouse models PMID: 27708283
  4. this study shows that human NAIP-NLRC4-inflammasome senses the Pseudomonas aeruginosa T3SS inner-rod protein PMID: 28992059
  5. mRNA expression levels of NLRP1 and NLRC4 were not altered in chronic hepatitis B patients, suggesting that these genes are not responsible for the impaired immune responses against HBV observed in these patients. PMID: 27750030
  6. The authors describe a novel mutation in NLRC4 in a large pedigree causing an NLRC4-associated, partially anakinra-responsive AID, dominated by cutaneous erythematous nodes and urticarial rash, arthralgias, and late-onset enterocolitis. PMID: 27203668
  7. Results found that NLRC4 expression increased in for promoting DN progression and demonstrate NLRC4-driven IL-1beta production as critical for the progression of DN. PMID: 27706238
  8. Ubiquitin-tagged NLRC4 could induce cell death and activate caspase-8 independent of Ser(533) phosphorylation. Our PMID: 27974463
  9. LPS activates the MAPK pathway in macrophages, thus resulting in the upregulation of NLRC4; however, NLRC4 inhibits IL1beta and IL18 production, contributing to the anti-inflammatory response. PMID: 27175981
  10. High expression of NLRP3, NLRC4, and CASP1 in background non-tumorous liver is significantly correlated with poor prognosis of patients after resection of hepatocellular carcinoma. PMID: 28011505
  11. association of IL-18 levels with a single nucleotide polymorphism in the untranslated exon 2 of the inflammasome component NLRC4 PMID: 26362438
  12. While both contributing to pathogen clearance, NLRP3 more than NLRC4 contributes to deleterious inflammatory responses in cystic fibrosis and correlates with defective NLRC4-dependent IL-1Ra production. PMID: 26972847
  13. analysis of a subset of inflammasome receptors including NLRP3, NLRC4 and AIM2 that triggers formation of the micrometer-sized spherical supramolecular complex called the ASC speck PMID: 26258904
  14. Thus, pathogenic inflammasome activity during Candida infection is negatively regulated by the IL-22/NLRC4/IL-1Ra axis. PMID: 26269955
  15. cN-II co-immunoprecipitated both with wild type Ipaf and its LRR domain after transfection with corresponding expression vectors, but not with Ipaf lacking the LRR domain. PMID: 25811392
  16. Elevated expression of IPAF was associated with inflammation in patients with pemphigus vulgaris disease. PMID: 25342284
  17. Data indicate that NLR family CARD domain containing 4 protein (NLRC4) is a causative gene for familial cold autoinflammatory syndrome (FCAS). PMID: 25385754
  18. Data show that the disease was caused by a de novo gain-of-function mutation in NLR family CARD domain containing 4 (NLRC4) encoding a p.Val341Ala substitution. PMID: 25217960
  19. inflammasome (also known as NLRC4) has been demonstrated to play a role in host defense mechanisms against pathogen-associated molecules PMID: 24054992
  20. This review describes the surprisingly diverse mechanisms by which NLRC4 senses bacteria and initiates innate immune responses. PMID: 23215645
  21. the NAIP5-NLRC4 inflammasome is induced by direct interactions with conserved N- and C-terminal regions of flagellin PMID: 23012363
  22. NLRC4 is important for host survival and bacterial clearance, as well as neutrophil-mediated inflammation in the lungs following Klebsiella pneumoniae infection. PMID: 22547706
  23. Twelve single nucleotide polymorphisms within NLRP1, NLRP3, NLRC4, CARD8, CASP1, and IL1B genes were analyzed in 150 HIV-1-infected Brazilian subjects. PMID: 22227487
  24. Genetic analysis of Chromobacterium violaceum infection revealed that the TTSS needle protein CprI can stimulate NLRC4 inflammasome activation in human macrophages PMID: 21918512
  25. [review] role of NLRC4 inflammasome and pathways in innate immune detection of bacterial virulence factors PMID: 20349122
  26. Apaf-1 protein expression did not correlate with Apaf-1 mRNA levels in human leukaemic blasts.Apaf-1 DNA promoter methylation might contribute to the inactivation of Apaf-1 expression PMID: 12545166
  27. CLAN regulates several events central to host defense mechanisms against invading bacteria: modulating relative sensitivity of macrophages to lipopolysaccharide and peptidoglycan, and impacting host-pathogen interactions. PMID: 15528373
  28. p53 can directly induce Ipaf gene transcription, which contributes to p53-dependent apoptosis in at least some human cells PMID: 15580302
  29. Involvement of caspase 1 and its activator Ipaf upstream of mitochondrial events in apoptosis PMID: 16817903
  30. hNAIP and hIpaf mediate innate intracellular defense against flagellated Legionella in human cells. PMID: 18453601

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Involvement in disease Autoinflammation with infantile enterocolitis (AIFEC); Familial cold autoinflammatory syndrome 4 (FCAS4)
Subcellular Location Cytoplasm, Cytoplasm, cytosol
Tissue Specificity Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isofo
Database Links

HGNC: 16412

OMIM: 606831

KEGG: hsa:58484

STRING: 9606.ENSP00000354159

UniGene: Hs.574741


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