Recombinant Human Polypyrimidine tract-binding protein 3 (PTBP3)

Code CSB-YP020060HU
MSDS
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Source Yeast
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Code CSB-EP020060HU
MSDS
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Source E.coli
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Code CSB-EP020060HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP020060HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP020060HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PTBP3
Uniprot No.
Alternative Names
DKFZp781I1117; Fission yeast differentiation regulator; OTTHUMP00000021931; OTTHUMP00000021932; Polypyrimidine tract binding protein 3; regulator of differentiation 1; Regulator of differentiation (in S. pombe) 1; Regulator of differentiation 1; ROD 1; Rod1; ROD1 regulator of differentiation 1; ROD1_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-552
Target Protein Sequence
MDGVVTDLIT VGLKRGSDEL LSSGIINGPF TMNSSTPSTA NGNDSKKFKR DRPPCSPSRV LHLRKIPCDV TEAEIISLGL PFGKVTNLLM LKGKSQAFLE MASEEAAVTM VNYYTPITPH LRSQPVYIQY SNHRELKTDN LPNQARAQAA LQAVSAVQSG SLALSGGPSN EGTVLPGQSP VLRIIIENLF YPVTLEVLHQ IFSKFGTVLK IITFTKNNQF QALLQYADPV NAHYAKMALD GQNIYNACCT LRIDFSKLTS LNVKYNNDKS RDFTRLDLPT GDGQPSLEPP MAAAFGAPGI ISSPYAGAAG FAPAIGFPQA TGLSVPAVPG ALGPLTITSS AVTGRMAIPG ASGIPGNSVL LVTNLNPDLI TPHGLFILFG VYGDVHRVKI MFNKKENALV QMADANQAQL AMNHLSGQRL YGKVLRATLS KHQAVQLPRE GQEDQGLTKD FSNSPLHRFK KPGSKNFQNI FPPSATLHLS NIPPSVTVDD LKNLFIEAGC SVKAFKFFQK DRKMALIQLG SVEEAIQALI ELHNHDLGEN HHLRVSFSKS TI
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
RNA-binding protein that mediates pre-mRNA alternative splicing regulation. Plays a role in the regulation of cell proliferation, differentiation and migration. Positive regulator of EPO-dependent erythropoiesis. Participates in cell differentiation regulation by repressing tissue-specific exons. Promotes FAS exon 6 skipping. Binds RNA, preferentially to both poly(G) and poly(U).
Gene References into Functions
  1. Low PTBP1 expression is associated with Prostate Cancer Growth. PMID: 29187400
  2. High PTBP3 expression is associated with Epithelial-Mesenchymal Transition in Breast Cancer. PMID: 29187406
  3. RTqPCR revealed that the expression level of miR210 and ROD1 in the antimiR210 group was significantly reduced when compared with the NC and BL groups (P<0.05). MiR210 was overexpressed in the serum of prostate cancer patients and transfection with an miR210 inhibitor was able to effectively inhibit the proliferation of prostate cancer cells and promote apoptosis. PMID: 29901117
  4. Increased PTBP3 expression is associated with 5- fluorouracil resistance in gastric cancer. PMID: 28222070
  5. We propose that miR-210 regulates cell proliferation and apoptosis in glioblastoma multiforme cells by targeting ROD1 PMID: 26443314
  6. Data show that polypyrimidine tract-binding proteins nPTB and ROD1 interact with mitochondrial tRNA(Thr) in the cytoplasm outside of mitochondria. PMID: 26657638
  7. PTBP3 may promote proliferation and inhibit the differentiation of human gastric cancer MKN45 cells PMID: 25119103
  8. ROD1 is a bona fide seedless target of miR-210. PMID: 23024754
  9. ROD1 and UPF1 associate with a significant number of common transcripts. PMID: 22575643
  10. Regulator of differentiation 1 (ROD1) binds to the amphipathic C-terminal peptide of thrombospondin-4 and is involved in its mitogenic activity. PMID: 19441079

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Tissue Specificity
Expressed in several hematopoietic cell lines examined.
Database Links

HGNC: 10253

OMIM: 607527

KEGG: hsa:9991

STRING: 9606.ENSP00000334499

UniGene: Hs.269988

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