PTBP3 Antibody

Datasheet
Code CSB-PA020060NA01HU
Size US$299
Uniprot No. O95758
Image
  • Western Blot
    Positive WB detected in: K562 whole cell lysate, MCF-7 whole cell lysate
    All lanes: PTBP3 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 60, 57, 61, 50 kDa
    Observed band size: 60 kDa

  • IHC image of CSB-PA020060NA01HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunoprecipitating PTBP3 in A549 whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-PA020060NA01HU in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000)
    Lane 2: CSB-PA020060NA01HU (5µg) + A549 whole cell lysate (0.5mg)
    Lane 3: A549 whole cell lysate (20µg)

Immunogen Peptide sequence from Human Polypyrimidine tract-binding protein 3 protein (37-55AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IP; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000
Relevance RNA-binding protein that mediates pre-mRNA alternative splicing regulation. Plays a role in the regulation of cell proliferation, differentiation and migration. Positive regulator of EPO-dependent erythropoiesis. Participates in cell differentiation regulation by repressing tissue-specific exons. Promotes FAS exon 6 skipping. Binds RNA, preferentially to both poly(G) and poly(U).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias Polypyrimidine tract-binding protein 3 (Regulator of differentiation 1) (Rod1), PTBP3, ROD1
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Research Area Epigenetics and Nuclear Signaling
Target Names PTBP3
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function RNA-binding protein that mediates pre-mRNA alternative splicing regulation. Plays a role in the regulation of cell proliferation, differentiation and migration. Positive regulator of EPO-dependent erythropoiesis. Participates in cell differentiation regulation by repressing tissue-specific exons. Promotes FAS exon 6 skipping. Binds RNA, preferentially to both poly(G) and poly(U).
Tissue Specificity Expressed in several hematopoietic cell lines examined.
Database Links

HGNC: 10253

OMIM: 607527

KEGG: hsa:9991

STRING: 9606.ENSP00000334499

UniGene: Hs.269988

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