Name: Recombinant Human Protein AATF(AATF),partial
Brand: CUSABIO
SKU: CSB-RP052644h

Product Details

Purity	Greater than 90% as determined by SDS-PAGE.
Target Names	AATF
Uniprot No.	Q9NY61
Research Area	Epigenetics and Nuclear Signaling
Alternative Names	AATF; AATF_HUMAN; Apoptosis antagonizing transcription factor ; Apoptosis-antagonizing transcription factor; BFR2; CHE 1; CHE1; DED; Protein AATF; Rb binding protein Che 1; Rb-binding protein Che-1
Species	Homo sapiens (Human)
Source	E.coli
Expression Region	6-294aa
Target Protein Sequence	PLALQLEQLLNPRPSEADPEADPEEATAARVIDRFDEGEDGEGDFLVVGSIRKLASASLLDTDKRYCGKTTSRKAWNEDHWEQTLPGSSDEEISDEEGSGDEDSEGLGLEEYDEDDLGAAEEQECGDHRESKKSRSHSAKTPGFSVQSISDFEKFTKGMDDLGSSEEEEDEESGMEEGDDAEDSQGESEEDRAGDRNSEDDGVVMTFSSVKVSEEVEKGRAVKNQIALWDQLLEGRIKLQKALLTTNQLPQPDVFPLFKDKGGPEFSSALKNSHKALKALLRSLVGLQE Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight	58.8kDa
Protein Length	Partial
Tag Info	N-terminal GST-tagged
Form	Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs	Protein FAQs
Storage Condition	Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time	Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA	Please contact us to get it.

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Target Data

Function	May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
Gene References into Functions	Results show that Che-1 protects colon cancer cells from apoptosis induced by hypoxia through its ability to regulate HIF1-alpha stabilization in colorectal cancer cells. PMID: 28214471 Results show that eEF1Bgamma binds to the Che-1 promoter region and its transcript, and describe a novel mitochondrial localization for the Che-1 protein which needs mitochondrial integrity for correct localization. PMID: 27639846 we identified the ANN complex as a novel functional module supporting the nucleolar maturation of 40S ribosomal subunits. Our data help to explain the described role of AATF in cell proliferation during mouse development as well as its requirement for malignant tumor growth. PMID: 27599843 the effect of APOBEC3G over-expression upon AATF gene expression, was examined. PMID: 27611213 loss of Che-1 inhibits proliferation and promotes apoptosis in MG-63 cells by decreasing the level of mutant p53 PMID: 27012205 It was concluded that PARP-1 was involved in the DNA damage repair induced by HQ via increasing the accumulation of apoptosis antagonizing transcription factor through PARylation. PMID: 26822515 These results confirm Che-1 as an important regulator of p53 activity and suggest Che-1 to be a promising yet attractive drug target for cancer therapy. PMID: 25996291 In the face of high glucose threat, mitochondrial UCP2 gene expression is regulated by miR-2909 and AATF. PMID: 25976474 This mutant AATF along with its interactome consisting of SP1, DNMT3B and Par-4 ensures cancer cell DNA methylation required for down-regulation of tumor suppressor genes. PMID: 25231211 HIPK2 depletion strongly decreases Che-1 ubiquitylation and degradation. PMID: 25210797 Che-1 expression correlates with the progression of multiple myeloma and is required for cell growth and survival.Che-1 controls mTOR through the induction of Redd1 and Deptor, two important repressors of mTOR. PMID: 25770584 Cell proliferation decreased by 41% which was accompanied by apoptosis induction in 30% MCF-7 cells after AATF gene knockdown. PMID: 23801113 Che-1 depletion abolishes the ability of Chk1 to bind pericentrin and to localize at centrosomes, which, in its turn, deregulates the activation of centrosomal cyclin B-Cdk1 and advances entry into mitosis. PMID: 23798705 These results identify AATF as a nucleolar-confined c-Jun cofactor whose expression levels and spatial distribution determine the stress-induced activity of c-Jun and the levels of c-Jun-mediated apoptosis. PMID: 22933572 Nuclear AATF enrichment is selected for in p53-proficient endometrial cancers. Focal copy number AATF gains correlate with reduced overall survival in neuroblastoma. AATF is a critical repressor of p53-driven apoptosis. PMID: 22909821 found that Che-1 is required for sustaining mutant p53 expression in several cancer cell lines, and that Che-1 depletion by siRNA induces apoptosis both in vitro and in vivo PMID: 20708154 No evidence for the association of mutations with breast cancer was observed. PMID: 20025740 Che-1 affects cell growth by interfering with the recruitment of HDAC1 by retinoblastoma protein. Che-1 overexpression activates DNA synthesis in quiescent NIH-3T3 cells through HFDAC1 displacement. PMID: 12450794 Che-1 can be considered a general HDAC1 competitor and its down-regulation is involved in colon carcinoma cell proliferation PMID: 12847090 apoptosis-antagonizing transcription factor binds to TSG101 in a process that enhances androgen receptor-mediated transcription by promoting its monoubiquitination PMID: 14761944 AATF gene may be of crucial importance in maintaining the leukemic state of a cell compartment through its ability to initiate cell proliferation coupled with repression of cellular apoptosis. PMID: 17006618 Che-1 as a new Pin1 and HDM2 target and confirm its important role in the cellular response to DNA damage. PMID: 17468107 Che-1 interacts with NRAGE and NRAGE overexpression downregulates endogenous Che-1 by targeting it for proteasome-dependent degradation. PMID: 17488777 This review explains the novel miRNA encoded exclusively by HIV-1 genome that has the ability to specifically target cellular AATF gene known to play a crucial role in the maintenance of adaptive immunity at nucleic acid level against HIV-1 invasion. PMID: 18341201 Show More Hide All
Subcellular Location	Nucleus, nucleolus
Protein Families	AATF family
Tissue Specificity	Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
Database Links	HGNC: 19235 OMIM: 608463 KEGG: hsa:26574 STRING: 9606.ENSP00000225402 UniGene: Hs.195740

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Check Code：

Code	CSB-RP052644h
Size	US$1726
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Protein AATF(AATF),partial

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Target Data

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