AATF Antibody

Code CSB-PA05264A0Rb
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • IHC image of CSB-PA05264A0Rb diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) AATF Polyclonal antibody
Uniprot No. Q9NY61
Target Names AATF
Alternative Names AATF antibody; AATF_HUMAN antibody; Apoptosis antagonizing transcription factor antibody; Apoptosis-antagonizing transcription factor antibody; BFR2 antibody; CHE 1 antibody; CHE1 antibody; DED antibody; Protein AATF antibody; Rb binding protein Che 1 antibody; Rb-binding protein Che-1 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Protein AATF protein (6-294AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:200-1:500
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
Gene References into Functions
  1. Results show that Che-1 protects colon cancer cells from apoptosis induced by hypoxia through its ability to regulate HIF1-alpha stabilization in colorectal cancer cells. PMID: 28214471
  2. Results show that eEF1Bgamma binds to the Che-1 promoter region and its transcript, and describe a novel mitochondrial localization for the Che-1 protein which needs mitochondrial integrity for correct localization. PMID: 27639846
  3. we identified the ANN complex as a novel functional module supporting the nucleolar maturation of 40S ribosomal subunits. Our data help to explain the described role of AATF in cell proliferation during mouse development as well as its requirement for malignant tumor growth. PMID: 27599843
  4. the effect of APOBEC3G over-expression upon AATF gene expression, was examined. PMID: 27611213
  5. loss of Che-1 inhibits proliferation and promotes apoptosis in MG-63 cells by decreasing the level of mutant p53 PMID: 27012205
  6. It was concluded that PARP-1 was involved in the DNA damage repair induced by HQ via increasing the accumulation of apoptosis antagonizing transcription factor through PARylation. PMID: 26822515
  7. These results confirm Che-1 as an important regulator of p53 activity and suggest Che-1 to be a promising yet attractive drug target for cancer therapy. PMID: 25996291
  8. In the face of high glucose threat, mitochondrial UCP2 gene expression is regulated by miR-2909 and AATF. PMID: 25976474
  9. This mutant AATF along with its interactome consisting of SP1, DNMT3B and Par-4 ensures cancer cell DNA methylation required for down-regulation of tumor suppressor genes. PMID: 25231211
  10. HIPK2 depletion strongly decreases Che-1 ubiquitylation and degradation. PMID: 25210797
  11. Che-1 expression correlates with the progression of multiple myeloma and is required for cell growth and survival.Che-1 controls mTOR through the induction of Redd1 and Deptor, two important repressors of mTOR. PMID: 25770584
  12. Cell proliferation decreased by 41% which was accompanied by apoptosis induction in 30% MCF-7 cells after AATF gene knockdown. PMID: 23801113
  13. Che-1 depletion abolishes the ability of Chk1 to bind pericentrin and to localize at centrosomes, which, in its turn, deregulates the activation of centrosomal cyclin B-Cdk1 and advances entry into mitosis. PMID: 23798705
  14. These results identify AATF as a nucleolar-confined c-Jun cofactor whose expression levels and spatial distribution determine the stress-induced activity of c-Jun and the levels of c-Jun-mediated apoptosis. PMID: 22933572
  15. Nuclear AATF enrichment is selected for in p53-proficient endometrial cancers. Focal copy number AATF gains correlate with reduced overall survival in neuroblastoma. AATF is a critical repressor of p53-driven apoptosis. PMID: 22909821
  16. found that Che-1 is required for sustaining mutant p53 expression in several cancer cell lines, and that Che-1 depletion by siRNA induces apoptosis both in vitro and in vivo PMID: 20708154
  17. No evidence for the association of mutations with breast cancer was observed. PMID: 20025740
  18. Che-1 affects cell growth by interfering with the recruitment of HDAC1 by retinoblastoma protein. Che-1 overexpression activates DNA synthesis in quiescent NIH-3T3 cells through HFDAC1 displacement. PMID: 12450794
  19. Che-1 can be considered a general HDAC1 competitor and its down-regulation is involved in colon carcinoma cell proliferation PMID: 12847090
  20. apoptosis-antagonizing transcription factor binds to TSG101 in a process that enhances androgen receptor-mediated transcription by promoting its monoubiquitination PMID: 14761944
  21. AATF gene may be of crucial importance in maintaining the leukemic state of a cell compartment through its ability to initiate cell proliferation coupled with repression of cellular apoptosis. PMID: 17006618
  22. Che-1 as a new Pin1 and HDM2 target and confirm its important role in the cellular response to DNA damage. PMID: 17468107
  23. Che-1 interacts with NRAGE and NRAGE overexpression downregulates endogenous Che-1 by targeting it for proteasome-dependent degradation. PMID: 17488777
  24. This review explains the novel miRNA encoded exclusively by HIV-1 genome that has the ability to specifically target cellular AATF gene known to play a crucial role in the maintenance of adaptive immunity at nucleic acid level against HIV-1 invasion. PMID: 18341201

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Subcellular Location Nucleus, nucleolus
Protein Families AATF family
Tissue Specificity Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
Database Links

HGNC: 19235

OMIM: 608463

KEGG: hsa:26574

STRING: 9606.ENSP00000225402

UniGene: Hs.195740

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