Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

PPM1A

Uniprot No.

P35813

Research Area

Signal Transduction

Alternative Names

Protein phosphatase 2C isoform alpha; EC 3.1.3.16; FLJ42306; IA; MGC9201; Mpp alpha ; PP2C alpha; PP2C-alpha; PP2CA; PP2Calpha; PPM 1A; PPM1A; PPM1A_HUMAN; PPPM1A; Protein phosphatase 1A (formerly 2C) magnesium dependent alpha isoform; Protein phosphatase 1A; Protein phosphatase 1A magnesium dependent alpha ; Protein phosphatase 2C alpha; Protein phosphatase 2C alpha isoform; Protein phosphatase 2C isoform alpha; Protein phosphatase IA; Protein phosphatase, Mg2+/Mn2+ dependent, 1A

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-382aa

Target Protein Sequence

GAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLESWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFKGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILICFPNAPKVSPEAVKKEAELDKYLECRVEEIIKKQGEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSTSTDDMW
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

69.3kDa

Protein Length

Full Length

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This Human PPM1A recombinant protein was produced in E.coli, where the gene sequence encoding Human PPM1A (1-382aa) was expressed with the N-terminal GST tag. The purity of this PPM1A protein was greater than 90% by SDS-PAGE.
PPM1A is a serine/threonine protein phosphatase, and its primary function is to remove phosphate groups from proteins, thereby regulating the activity of cellular signaling pathways. It participates in several critical biological processes by dephosphorylating proteins, including cell growth, cell differentiation, apoptosis, and cell cycle control.
PPM1A plays a role in regulating various signaling pathways. For example, it can modulate the Protein Kinase B (PKB or Akt) signaling pathway, which is important for cell survival and proliferation. Additionally, PPM1A can regulate the Nuclear Factor-κB (NF-κB) signaling pathway, which is involved in immune responses and inflammation.
Abnormal expression or mutations in PPM1A may be associated with the development of various diseases. For instance, some studies have suggested that abnormal expression of PPM1A is linked to the onset and growth of tumors, implying its potential role in the pathogenesis of cancer. Furthermore, PPM1A may also be associated with neurological disorders, cardiovascular diseases, and several other conditions.

Target Background

Function

Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.

Gene References into Functions

PPM1A as a negative threshold regulator of M1-type monocyte-to-macrophage differentiation. PMID: 29343725
Here the authors establish PPM1A as a checkpoint target used by Mycobacterium tuberculosis to suppress macrophage apoptosis. Overproduction of PPM1A suppressed apoptosis of Mycobacterium tuberculosis-infected macrophages by a mechanism that involves inactivation of the c-Jun N-terminal kinase (JNK). PMID: 28176854
Present study suggests that HBx-induced degradation of PPM1a is a novel mechanism for over-activation of TGF-beta pathway in HCC development. PMID: 27121309
Findings show that HCV infection and replication decreased PPM1A abundance, mediated by NS3, in hepatoma cells. Compared to normal liver tissues, the expression of PPM1A was significantly decreased in the HCC tumor tissues and adjacent non-tumor tissues through its regulation by NS3 which promotes its ubiquitination and proteasomal degradation. PMID: 28283039
These data suggest that PPM1A, which had previously been shown to play a role in the antiviral response to Herpes Simplex virus infection, also governs the antibacterial response of macrophages to bacteria, or at least to Mycobacterium tuberculosis infection PMID: 27004401
establish PPM1A as a novel repressor of the SMAD3 pathway in renal fibrosis PMID: 27328942
Report a tumor-suppressive function of PPM1A and an independent relationship to Smad4 in pancreatic ductal adenocarcinoma. PMID: 27195906
hydrogen/deuterium exchange-mass spectrometry and molecular dynamics to characterize conformational changes in PP2Calpha between the active and inactive states PMID: 28481111
In a nested case control study of ischemic stroke, there was an epigenome-wide association for cg04985020 (PPM1A; P=1.78x10(-07)) with vascular recurrence in patients treated with aspirin. PMID: 27301936
findings demonstrate a novel regulatory circuit in which STING and TBK1 reciprocally regulate each other to enable efficient antiviral signaling activation, and PPM1A dephosphorylates STING and TBK1 PMID: 25815785
The TGF-beta/Smad signaling system decreases its activity through strong negative regulation. We provide evidence for a new negative feedback loop through PPM1A upregulation. PMID: 24901250
Loss of PPM1A is associated with the development of tumor invasion in bladder cancer patients. PMID: 25026293
PPM1A is a RelA phosphatase that regulates NF-kappaB activity and that PPM1A has tumor suppressor-like activity. PMID: 23812431
a nuclear envelope-localized mechanism of inactivating TGF-beta signaling in which MAN1 competes with transcription factors for binding to Smad2 and Smad3 and facilitates their dephosphorylation by PPM1A. PMID: 23779087
phosphatase activity toward phosphopeptide substrates by PP2Calpha and Wip1 requires the binding of a Mg(2)+ ion to the low-affinity site. PMID: 23906386
PPM1A negatively regulates ERK by directly dephosphorylating its pThr202 position early in epidermal growth factor stimulation. Additional kinetic studies reveal that key residues participate in phospho-ERK recognition by PPM1A. PMID: 23560844
Studies indicate that phosphatase PPM1G is a component of the spliceosome and binds to protein YB-1 to affect alternative splicing. PMID: 22519956
PPM1A inhibits HIV-1 infection and gene expression. PPM1A depletion in resting CD4+ T cells increases HIV-1 gene expression. PMID: 22727189
High expression of LMP2 and low expression of PPM1A might play an important role in the motility and invasiveness of trophoblast cells and malignant transformation of hydatidiform mole. PMID: 22041443
This work demonstrates that PPM1A/PP2Calpha, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFbeta signaling. PMID: 16751101
PPM1A plays an important role in controlling BMP signaling through catalyzing Smad1 dephosphorylation PMID: 16931515
Aberrant location of expression/staining intensity of PTEN, PPM1A and P-Smad2 in hepatocellular carcinoma may impact disease progression. PMID: 17729405
PTEN abrogates TGF-beta-induced Smad2/3 phosphorylation. This study establishes a novel role for nuclear PTEN in the stabilization of PPM1A. PMID: 18482992
Overexpression of PPM1A and the related PPM1B greatly reduced Cdk9 T-loop phosphorylation PMID: 18829461
Protein phosphatase 1A is essential to terminate I-kappa B Kinase-mediated NF-kappaappaB activation through binding to the activated form of I-kappa B Kinase and dephosphorylating I-kappa B Kinase at the conserved residues Ser177 and Ser181. PMID: 18930133
The present data indicate that PPM1A plays a critical role in the regulation of normal placentation by inhibiting trophoblast migration and invasion. PMID: 19404668

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Subcellular Location

Nucleus. Cytoplasm, cytosol. Membrane; Lipid-anchor.

Protein Families

PP2C family

Database Links

HGNC: 9275

OMIM: 606108

KEGG: hsa:5494

STRING: 9606.ENSP00000327255

UniGene: Hs.130036

Code	CSB-EP018489HU
Abbreviation	Recombinant Human PPM1A protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Protein phosphatase 1A (PPM1A)

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