Recombinant Human Ribosomal protein S6 kinase alpha-5 (RPS6KA5), partial

Code CSB-YP020468HU
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Source Yeast
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Code CSB-EP020468HU
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Source E.coli
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Code CSB-EP020468HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP020468HU
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Source Baculovirus
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Code CSB-MP020468HU
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Source Mammalian cell
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Product Details

>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
90 kDa ribosomal protein S6 kinase 5; EC ; KS6A5_HUMAN; MGC1911; Mitogen and stress activated protein kinase 1; MSPK1; Nuclear Mitogen And Stress Activated Protein Kinase 1; Nuclear mitogen- and stress-activated protein kinase 1; Ribosomal protein S6 kinase 90kD polypeptide 5; Ribosomal protein S6 kinase 90kDa; Ribosomal protein S6 kinase 90kDa polypeptide 5; Ribosomal Protein S6 Kinase Alpha 5; Ribosomal protein S6 kinase alpha-5; RLPK ; RPS6KA5; RSK Like Protein Kinase; RSK-like protein kinase; RSKL; S6K alpha 5; S6K-alpha-5
Homo sapiens (Human)
Protein Length
Tag Info
The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Please contact us to get it.

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Target Background

Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression. In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress. In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential. Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors. Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury. Phosphorylates TRIM7 at 'Ser-107' in response to growth factor signaling via the MEK/ERK pathway, thereby stimulating its ubiquitin ligase activity.
Gene References into Functions
  1. MSK1 was overexpressed in 148 out of 329 colorectal cancer (CRC) patients. CRC patients with high MSK1 expression had shorter overall survival than those with low MSK1, especially among patients with stage III tumors. Overexpression of MSK1 is associated with poor prognosis in CRC and is connected to tumor aggressiveness. PMID: 28314603
  2. High MSK1 is associated with improved breast cancer-specific survival in early stage invasive breast cancer patients, and has additional prognostic value in HER2-negative and non-basal like disease. PMID: 29327245
  3. Our findings indicate that MSK1/beta-catenin signaling serves as an escape survival signal upon PI3K inhibition and provides a strong rationale for the combined use of PI3K and MSK1/beta-catenin inhibition to induce lethal growth inhibition in human GBM cells. PMID: 27196759
  4. Results show that MSK1 phosphorylates H3S10 through p38-MAPK pathway in gastric cancer patients. PMID: 27588146
  5. Interrupting MSK1 activation is a new target for antioxidants. PMID: 26030901
  6. Increased MSK1 activity is critically important for Epstein-Barr virus LMP1-promoted cell proliferation and transformation. PMID: 25958199
  7. Authors conclude that paramyxoviruses trigger the DNA damage response, a pathway required for MSK1 activation of phospho Ser 276 RelA formation to trigger the IRF7-RIG-I amplification loop necessary for mucosal interferon production. PMID: 25520509
  8. These results highlight the relevance of MSK1 in the up-regulation of RARbeta by prostaglandin E2. PMID: 24953041
  9. MSK1 plays an important role for hormone-dependent breast cancer growth PMID: 23604116
  10. Angiopoietin 2-mediated signaling via survivin/ref-1/MSK-1 pathway promotes doxorubicin resistance in HepG2 cells. PMID: 23643942
  11. Astaxanthin attenuates the UVB-induced secretion of prostaglandin E2 and interleukin-8 in human keratinocytes by interrupting MSK1 phosphorylation in a ROS depletion-independent manner PMID: 22626465
  12. MSK1 is an important downstream kinase involved in CS-induced NF-kappaB activation PMID: 22312446
  13. MSK1 is a direct and potent transcriptional activator when targeted to c-fos promotor, and when targeted to alpha-globin promoter induces H3 S28 phosphorylation reactivating expression of this polycomb-silenced gene. PMID: 21282660
  14. In this study, by applying a novel method, we have identified the phosphorylation sites in human MSK1 mitogen- and stress-activated protein kinase 1, and show that MRK-beta could also activate MSK1 through direct interaction. PMID: 20408143
  15. MiR-148a attenuates paclitaxel resistance of hormone-refractory, drug-resistant prostate cancer PC3 cells by regulating MSK1 expression PMID: 20406806
  16. Bile acid regulates MUC2 transcription in colon cancer cells via positive EGFR/PKC/Ras/ERK/CREB, PI3K/Akt/IkappaB/NF-kappaB and p38/MSK1/CREB pathways and negative JNK/c-Jun/AP-1 pathway. PMID: 20198339
  17. The MSK1 can be phosphorylated and activated in cells by both ERK1/2 and p38alpha. PMID: 20044958
  18. Data provide evidence that p38 Map kinase pathway is activated leading to increased upregulation of mixed lineage kinase 3, MKK3/6, MSK1, and Mapkapk2, upon treatment of BCR/ABL expressing cells with dasatinib. PMID: 19672773
  19. IL1beta and TNFalpha activation of MSK1 and CREB and cAMP-response element signaling cascades occurs via ERK/p38 MAP kinases and are crucial aspects of the intracellular mechanisms that mediate MUC5AC gene expression PMID: 12690113
  20. acetylation of histones may stimulate transcription by suppressing an inhibitory phosphorylation by MSK1 PMID: 15010469
  21. MSK1 has a role in transforming growth factor-beta-mediated responses through p38alpha and Smad signaling pathways PMID: 15133024
  22. essential role of the C-terminal domain of MSK1 for its constitutive interaction with group V secretory phospholipase A(2), which appears essential to support VEGF-mediated PAF synthesis PMID: 16479592
  23. Plays a positive role in the control of cell proliferation of both HaCaT keratinocytes and the A431 human epidermoid carcinoma line. PMID: 16532028
  24. As MSK1 regulates the production of pro-inflammatory cytokines, it may play a role in the pathogenesis of psoriasis. PMID: 16543895
  25. MSK1 has a role downstream of p38 in the regulation of As2O3 responses PMID: 16762916
  26. Thr700 phosphorylation relieves the inhibition of MSK1 by a C-terminal autoinhibitory helix and helps induce a conformational shift that protects Thr581 from dephosphorylation PMID: 17117922
  27. the induction of inflammatory genes by farnesol is mediated by the activation of the NF-kappaB pathway and involves MEK1/2-ERK1/2-MSK1-dependent phosphorylation of p65/RelA(Ser(276)) PMID: 18424438
  28. This study provides therefore a direct link between MSK1-mediated phosphorylation of Ser276 p65 of NF-kappaB, allowing its binding to the SCF intronic enhancer, and pathophysiological SCF expression in inflammation. PMID: 19197368
  29. IL-17F may be involved in airway inflammation and remodeling via the induction of IL-11, and RafI-MEK1/2-ERK1/2-MSK1-CREB is identified as a novel signaling pathway. PMID: 19251839
  30. Data reveal that TPA activates transcription of TBX2 through activating MSK1, which leads to an increase in phosphorylated histone H3 and the recruitment of Sp1 to the TBX2 gene. PMID: 19633291
  31. We conclude that RSV induces RelA activation in the innate inflammatory response via a pathway separate from that controlling RelA cytoplasmic release, mediated by ROS signaling to cytoplasmic MSK1 activation and RelA Ser-276 phosphorylation. PMID: 19706715

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Subcellular Location
Nucleus. Cytoplasm. Note=Predominantly nuclear. Exported into cytoplasm in response to glucocorticoid.
Protein Families
Protein kinase superfamily, AGC Ser/Thr protein kinase family, S6 kinase subfamily
Tissue Specificity
Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.
Database Links

HGNC: 10434

OMIM: 603607

KEGG: hsa:9252

STRING: 9606.ENSP00000261991

UniGene: Hs.510225

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