Recombinant Human Zinc finger protein ZIC 2(ZIC2)

Code CSB-YP026485HU
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Source Yeast
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Code CSB-EP026485HU
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Source E.coli
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Code CSB-EP026485HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP026485HU
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Source Baculovirus
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Code CSB-MP026485HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ZIC2
Uniprot No. O95409
Alternative Names HPE 5; HPE5; Odd paired homolog Drosophila; Zic 2; Zic family member 2 (odd paired Drosophila homolog); Zic family member 2; ZIC2; ZIC2_HUMAN; Zinc finger protein of the cerebellum 2; Zinc finger protein ZIC 2; Zinc finger protein Zic2
Species Homo sapiens (Human)
Expression Region 1-532
Target Protein Sequence MLLDAGPQFP AIGVGSFARH HHHSAAAAAA AAAEMQDREL SLAAAQNGFV DSAAAHMGAF KLNPGAHELS PGQSSAFTSQ GPGAYPGSAA AAAAAAALGP HAAHVGSYSG PPFNSTRDFL FRSRGFGDSA PGGGQHGLFG PGAGGLHHAH SDAQGHLLFP GLPEQHGPHG SQNVLNGQMR LGLPGEVFGR SEQYRQVASP RTDPYSAAQL HNQYGPMNMN MGMNMAAAAA HHHHHHHHHP GAFFRYMRQQ CIKQELICKW IDPEQLSNPK KSCNKTFSTM HELVTHVSVE HVGGPEQSNH VCFWEECPRE GKPFKAKYKL VNHIRVHTGE KPFPCPFPGC GKVFARSENL KIHKRTHTGE KPFQCEFEGC DRRFANSSDR KKHMHVHTSD KPYLCKMCDK SYTHPSSLRK HMKVHESSPQ GSESSPAASS GYESSTPPGL VSPSAEPQSS SNLSPAAAAA AAAAAAAAAA VSAVHRGGGS GSGGAGGGSG GGSGSGGGGG GAGGGGGGSS GGGSGTAGGH SGLSSNFNEW YV
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Acts as a transcriptional activator or repressor. Plays important roles in the early stage of organogenesis of the CNS. Activates the transcription of the serotonin transporter SERT in uncrossed ipsilateral retinal ganglion cells (iRGCs) to refine eye-specific projections in primary visual targets. Its transcriptional activity is repressed by MDFIC. Involved in the formation of the ipsilateral retinal projection at the optic chiasm midline. Drives the expression of EPHB1 on ipsilaterally projecting growth cones. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'. Associates to the basal SERT promoter region from ventrotemporal retinal segments of retinal embryos.
Gene References into Functions
  1. Results show that ZIC2 is highly expressed in osteosarcoma cells and tissues, and its overexpression promotes viability, invasion of osteosarcoma cells via SHIP2 suppression, and PI3K/AKT activation. PMID: 28857346
  2. the studies confirm the extent of ZIC2 allelic heterogeneity and that pathogenic variants of ZIC2 are associated with both classic and middle interhemispheric variant (MIHV) HPE which arise from defective ventral and dorsal forebrain patterning, respectively. PMID: 29442327
  3. miR-1271/Zic2/PAK4 axis plays an important role in hepatocellular carcinoma progression. PMID: 28577975
  4. Together SMAD3 and ZIC2 regulate FOXA2 transcription in cultured cells and Zic2 also controls the foxA2 expression during Xenopus development. These findings reveal a new mechanism of NODAL signal transduction in the mammalian node and provide the first molecular explanation of how ZIC2 loss-of-function precipitates Holoprosencephaly (HPE PMID: 27466203
  5. Data show that Zinc finger protein ZIC 2 (ZIC2) is indispensable in the regulation of pancreatic ductal adenocarcinoma (PDAC) cell apoptosis. PMID: 26318045
  6. ZIC2 acts upstream of OCT4 and recruits the nuclear remodeling factor complex to the OCT4 promoter, initiating OCT4 activation. ZIC2 levels positively correlated to the clinicopathological stages of HCC patients. PMID: 26426078
  7. Mutational screening for HPE genes revealed the occurrence of a frameshift mutation in the ZIC2 gene. PMID: 24677696
  8. ZIC2 and RASGRF1 are susceptibility genes, not only for common myopia, but also for high myopia. PMID: 24150758
  9. The c.1401_1406dup (p.Ala469_470dup) or alanine tract expansion to 17 residues) in the ZIC2 gene are likely to be medically significant for holoprosencephaly in a Brazilian cohort. PMID: 22847929
  10. A high level of sequence variation in the 3'UTR of ZIC2 may be associated with holoprosencephaly. PMID: 22859937
  11. ZIC2, a transcription factor related to the sonic hedgehog pathway, is a strong discriminant between MAL and LMP tumors: it may be a major determinant of outcome of EOTs. PMID: 22733541
  12. Mutations in ZIC2 is associated with holoprosencephaly. PMID: 22310223
  13. Brain malformations, including neuronal migration defects, predominated in individuals with ZIC2 mutations. PMID: 21940735
  14. role for ZIC2 as a transcriptional regulator of the beta-catenin.TCF4 complex. PMID: 21908606
  15. Our findings suggest that Zic2 is a positive modulator increasing Gli1 transcriptional and oncogenic activity by retaining Gli1 in the nucleus of cervical cancer cells. PMID: 21661123
  16. mutation of ZIC2 is a rare cause of, or contributor to, RES associated with HPE. PMID: 21638761
  17. the smallest 13q deletion associated to DWM allows further narrowing of the previously established critical region for this brain malformation to 13q32.2-32.3. Among the few genes of the deleted region, ZIC2 and ZIC5 seem the most plausible candidates. PMID: 20683983
  18. screened four known HPE genes in a Dutch cohort of 86 non-syndromic HPE index cases, including 53 family members. We detected 21 mutations (24.4%), 3 in SHH, 9 in ZIC2 and 9 in SIX3 PMID: 20531442
  19. Holoprosencephaly due to ZIC2 mutations is distinct from that due to mutations in other genes. PMID: 19955556
  20. ZIC2 expression was associated with the histopathologic types of oral squamous cell carcinoma PMID: 19784848
  21. Sample was too small to reach definitive conclusions, but the evidence is sufficiently intriguing to encourage further research. PMID: 11857562
  22. Holoprosencephaly in monosomy 13q may be related to ZIC2 gene loss of function. PMID: 11910512
  23. Zic2 is an evolutionarily conserved determinant of retinal ganglion cells that project ipsilaterally PMID: 13678579
  24. Four novel ZIC2 mutations found in a cohort of Holoprosencephaly patinets. PMID: 15221788
  25. The C-terminal alanine-tract of ZIC2 influences the strength of DNA binding and alters transcriptional activity in a promoter-specific manner PMID: 15590697
  26. Forms two different complexes with DNA-dependent protein kinase, poly(ADP-ribose) polymerase, and RNA helicase A; all the components interacted directly with Zic2 protein PMID: 17251188
  27. This transcription factor regulates the expression of EphB1 in RGCs and also suggest the existence of an additional EphB1-independent pathway controlled by Zic2 that contributes to retinal axon divergence at the midline. PMID: 18417618
  28. ZIC2, SPRY2, and GPC5 genes are candidate genes suspected to explain the malformations associated with cerebral anomalies in the hypothesis of a contiguous gene syndrome in 13q deletion syndrome PMID: 19022413
  29. Loss-of-function as the likely pathogenetic mechanism common to most, if not all, of ZIC2 mutations in holoprosencephaly. PMID: 19177455

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Involvement in disease Holoprosencephaly 5 (HPE5)
Subcellular Location Nucleus. Cytoplasm.
Protein Families GLI C2H2-type zinc-finger protein family
Database Links

HGNC: 12873

OMIM: 603073

KEGG: hsa:7546

STRING: 9606.ENSP00000365514

UniGene: Hs.653700

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