Recombinant Lysinibacillus sphaericus Larvicidal toxin 51 kDa protein (binB)

Code CSB-YP321615LRU
MSDS
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Source Yeast
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Code CSB-EP321615LRU
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Source E.coli
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Code CSB-EP321615LRU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP321615LRU
MSDS
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Source Baculovirus
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Code CSB-MP321615LRU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
binB
Uniprot No.
Alternative Names
binB; sph04Binary larvicide subunit BinB; 51.4 kDa insecticidal toxin; BinB protein; Larvicidal toxin protein P51
Species
Lysinibacillus sphaericus (Bacillus sphaericus)
Expression Region
1-448
Target Protein Sequence
MCDSKDNSGV SEKCGKKFTN YPLNTTPTSL NYNLPEISKK FYNLKNKYSR NGYGLSKTEF PSSIENCPSN EYSIMYDNKD PRFLIRFLLD DGRYIIADRD DGEVFDEAPT YLDNNNHPII SRHYTGEERQ KFEQVGSGDY ITGEQFFQFY TQNKTRVLSN CRALDSRTIL LSTAKIFPIY PPASETQLTA FVNSSFYAAA IPQLPQTSLL ENIPEPTSLD DSGVLPKDAV RAVKGSALLP CIIVHDPNLN NSDKMKFNTY YLLEYKEYWH QLWSQIIPAH QTVKIQERTG ISEVVQNSMI EDLNMYIGAD FGMYFYLRSS GFKEQITRGL NRPLSQTPTQ LGERVEEMEY YNSNDLDVRY VKHALAREFT LKRVNGEIVK NWVAVDYRMA GIQSYPNAPI TNPLTLTKHT IIRCENSYDG HIFKTPLIFK NGEVIVKTNE ELIPKINQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of a binary toxin active against Culex and some Aedes mosquito larvae. This subunit is responsible for localized binding to specific regions of the host larval gut. The individual subunits are not toxic. BinAB and this subunit alone bind to the gastric caecum and posterior midgut of C.quinquefasciatus larvae. Binary toxin internalization into host gut cells requires both proteins. Does not bind to the midgut of Aedes aegypti. Toxic to Aedes atropalpus mosquito larvae; mortality towards both C.quinquefasciatus and A.atropalpus is maximal by 48 hours. A.aegypti is not very susceptible to this toxin. Binding component of binary toxin. The 51 kDa polypeptide acts synergetically with the 42 kDa polypeptide for expression of a larvicidal toxin.
Subcellular Location
Spore, perispore.
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