Recombinant Macaca fascicularis Aspartate aminotransferase, cytoplasmic (GOT1)

Code CSB-YP709094MOV
MSDS
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Source Yeast
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Code CSB-EP709094MOV
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Source E.coli
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Code CSB-EP709094MOV-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP709094MOV
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Source Baculovirus
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Code CSB-MP709094MOV
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GOT1
Uniprot No.
Alternative Names
GOT1; QccE-14568Aspartate aminotransferase; cytoplasmic; cAspAT; EC 2.6.1.1; EC 2.6.1.3; Cysteine aminotransferase; cytoplasmic; Cysteine transaminase; cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A
Species
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Expression Region
2-413
Target Protein Sequence
APPSVFSEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIIVLH ACAHNPTGTD PTPEQWKQIA SVMKHRFLFP FFDSAYQGFA SGNLERDAWA IRYFVSEGFE FFCAQSFSKN FGLYNERVGN LTVVGKEPES ILRVLSQMEK IVRITWSNPP AQGARIVADT LSNPELFEEW KGNVKTMADR ILTMRSELRA RLEALKTPGT WNHITDQIGM FSYTGLNPKQ VEYLINEKHI YLLPSGRINV SGLTTKNLDY VATSIHEAIT KIQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
Subcellular Location
Cytoplasm.
Protein Families
Class-I pyridoxal-phosphate-dependent aminotransferase family
Database Links

KEGG: mcf:101865666

UniGene: Mfa.8329

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