Recombinant Macaca mulatta Tripartite motif-containing protein 5 (TRIM5)

1. Data suggest that SPRY domain of TRIM5a is essential in activation of AP-1 signaling but not NF-kappaB signaling in this species. (TRIM5a = tripartite motif-containing 5 alpha isoenzyme; AP-1 = activator 1 protein) PMID: 29196608
2. High resolution crystal structure of a Trim5Alpha dimeric Bbox domain. PMID: 27402535
3. L2 region of the TRIM5alpha dimer undergoes dynamic conformational changes, which results in the displacement of L2 regions by 25 angstroms relative to each other. PMID: 27821283
4. HIV-1 virus target the rhTRIM5alpha enhanced the susceptibility to HIV-1 infection. PMID: 27888407
5. Mutations in gag and pol are associated with escape of HIV-1 from rhTrim5alpha. PMID: 26469551
6. These studies provide direct evidence that ubiquitin conjugation to rhTRIM5alpha-containing complexes is required for inhibition of HIV-1 Reverse Transcription and Capsid Destabilization. PMID: 26676782
7. These results confirm that the SUMO machinery is involved in TRIM5alpha-mediated retroviral restriction, and demonstrate that TRIM5alpha is a SUMO 1 and SUMO 2 substrate. PMID: 25880753
8. These data show that there could be some distinct HIV-1 capsid patterns to confer significant resistance to rhesus TRIM5-alpha. PMID: 25195168
9. Authors found that TRIM5alpha restriction significantly delayed disease progression and improved the survival rate of simian immunodeficiency virus-infected macaques. PMID: 25473059
10. Analysis of TRIM genotype revealed a potential role for TRIM in disease development in the central nervous system. 5 of 5 animals with the permissive TRIM5alpha genotype (TRIMQ/Q) progressed to Simian Immunodeficiency Virus Encephalitis. In contrast, 2 animals with the restrictive TRIM5alpha genotype (TRIMTFP/TFP) did not show neurologic signs, nor did they develop SIVE. PMID: 25187546
11. Data show that rhTRIM5alpha is stable and able to accumulate in cytoplasmic bodies, as well as to potently block HIV-1 infection. PMID: 24662946
12. Rhesus TRIM5alpha acts as an autophagic receptor to restrict HIV-1 through autophagic degradation. PMID: 25127057
13. In conclusion, the TRIM5alpha sumoylation site appears to modulate the E3 ubiquitin ligase activities of the adjacent RING domain, promoting K63-linked ubiquitin chains at the expense of auto-ubiquitylation which is probably K48-linked. PMID: 24583231
14. The antiparallel organization of the NtD regions of Fv1 and Trim5alpha dimers correctly positions C-terminal specificity and N-terminal effector domains and facilitates stable binding to adjacent capsid hexamers in viral cores. PMID: 24979782
15. Taken together, it is likely that rhTRIM5alpha cytoplasmic bodies are involved in recruiting components of the ubiquitin-proteasome system to coordinate proteasomal destruction of a viral or cellular protein(s) during restriction of HIV-1. PMID: 23799296
16. Assisted evolution enables HIV-1 to overcome a high TRIM5alpha-imposed genetic barrier to rhesus macaque tropism. PMID: 24086139
17. direct evidence that TRIM5alpha exerts selective pressure on the cross-species transmission of SIV in primates. PMID: 23990789
18. PRYSPRY domain serves an unknown function, distinct from the binding of TRIM5alpharh to the HIV-1 core. PMID: 24314652
19. influence of the TRIM5 genotype on susceptibility to rectal S(H)IV infection and on plasma viremia PMID: 23461569
20. Virus-specific effects of TRIM5alpha(rh) RING domain functions on restriction of retroviruses. PMID: 23637418
21. These results suggest that the rhTRIM5alpha B30.2/SPRY domain is not only important for the recognition of the HIV-1 CA, but it is also important for its association with SUMO-1 or SUMO-1 modified proteins. PMID: 23369348
22. The recognition of the entire capsid surface is a general strategy for TRIM5alpha to restrict murine leukemia viruses. PMID: 23536686
23. TRIMCyp individuals were identified in Chinese M. mulatta originating from Yunnan, although multiple earlier studies failed to find CypA retrotransposition in this subspecies. TRIMe7-CypA was expressed in M. mulatta. PMID: 23233150
24. Our results do not support a role for SUMO-interacting motifs or SUMOylation in the antiviral properties of TRIM5alpha. PMID: 23084420
25. Combined cryo-electron microscopy and biochemical data show that the monomeric rhesus TRIM5alpha PRY/SPRY, but not the human TRIM5alpha PRY/SPRY domain, can bind to HIV-1 capsid protein assemblies without causing disruption of the capsid. PMID: 23091002
26. TRIM5alpha utilizes proteasome during HIV-1 virus restriction. PMID: 22624877
27. A high-resolution structure of a TRIM5alpha PRYSPRY domain, the PRYSPRY of the rhesus monkey TRIM5alpha that potently restricts HIV infection, is reported. PMID: 22847415
28. results suggest that TRIM5alpha(rh) blocks HIV-1 reverse transcription by inducing premature viral uncoating in target cells. PMID: 22114335
29. TRIM5alpha represses HIV-1 LTR promoter activity by negatively regulating TAK1/TAB1/TAB2/TAB3-complex-mediated NF-kappaB activation. PMID: 21918865
30. The authors observed a significant effect of TRIM5 genotype on mucosal simian immunodeficiency virus SIVsmE660 acquisition in that no virus transmission occurred in monkeys with only restrictive TRIM5 alleles. PMID: 21775457
31. Animals coexpressing the TRIM5(TFP) and TRIM5(CypA) alleles took significantly longer to become infected with SIVsmE660, but not SIVmac239, after repeated limiting-dose intrarectal challenge. PMID: 21734037
32. Overall, these results demonstrate a role for TRIM5alpha self-ubiquitylation in the ability of TRIM5alpha to restrict HIV-1. PMID: 21734049
33. The authors discovered the ability of human and rhesus TRIM5alpha to shuttle into and out of the nucleus. PMID: 21575157
34. The B-box 2, coiled-coil and linker 2 regions of TRIM5a form a core dimerization motif that exhibits a high level of alpha-helical content. PMID: 21205312
35. TRIM5 can suppress SIV replication in vivo, exerting selective pressure during the initial stages of cross-species transmission. PMID: 20808775
36. G116E substitution in the capsid protein of the HIV-1 recombinant NL-4/5S6/7SvifS virus rendered the virus resistant to cynomolgus monkey, but not human TRIM5 alpha-mediated restriction. PMID: 20609213
37. Mamu-A*012 status was associated with significantly more frequent death (P = 0.04) as was TRIM5 alleles 6-11 PMID: 20585621
38. Collectively, these results suggest that TRIM5alpha-dependent restriction of HIV-1 infection results from structural perturbation of the viral capsid leading to aberrant HIV-1 uncoating in target cells. PMID: 20410272
39. These results suggest that Hsp70 plays critical roles in the cellular management against the TRIM5alpha-induced cellular insults. PMID: 20053985
40. Results suggest that the TRIM5alpharh-mediated late restriction involves (i) interaction with HIV-1 Gag polyprotein, and (ii) an effector function(s) that depends upon the coiled-coil and linker 2 domains of TRIM5alpharh. PMID: 19951947
41. inhibits herpes simplex virus at the early stage of infection PMID: 20060996
42. These findings demonstrate a central role for TRIM5alpha in limiting the replication of an immunodeficiency virus infection in a primate host. PMID: 20107597
43. TRIM5alpha blocks HIV-1 production by actively degrading the viral Gag protein. [TRIM5alpha] PMID: 17435772
44. Some alterations in the TRIM5alpha B-box 2 domain apparently affect the orientation or conformation of the B30.2(SPRY) domain, influencing capsid recognition. PMID: 17543365
45. We observe that proteasome inhibition allows HIV-1 to remain stably sequestered into large rhTRIM5alpha cytoplasmic bodies, preventing the clearance of HIV-1 viral complexes from the cytoplasm and revealing an intermediate in the restriction process. PMID: 18250195
46. lack of activity against HIV-1 suggests that Mamu7 homozygous animals will be null for TRIM5-mediated restriction of HIV-1 PMID: 18287035
47. We present here the NMR structure of the TRIM5 B-box 2 domain and identify an unusual hydrophobic patch on the domain surface important for self association, capsid binding avidity, and retrovirus restriction. PMID: 19656869

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KEGG: mcc:574288

STRING: 9544.ENSMMUP00000000454

UniGene: Mmu.3751