Recombinant Mouse BRCA1-A complex subunit RAP80 (Uimc1)

Code CSB-YP713181MO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP713181MO
MSDS
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Source E.coli
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Code CSB-EP713181MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP713181MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP713181MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uimc1
Uniprot No.
Alternative Names
Uimc1; Rap80; Rip110; Rxrip110; BRCA1-A complex subunit RAP80; Receptor-associated protein 80; Ubiquitin interaction motif-containing protein 1
Species
Mus musculus (Mouse)
Expression Region
1-727
Target Protein Sequence
MPRRKKKIKE ASESQNLEKK DLETSSCVSI KKKRRLEDLL IVISDSDGEE TKEENGLQKT KTKQSNRSKC LAKRKVAHMS EEEQFALALK MSEQEAREVN NQEEKEEELL RKAIAESLNS CWSSAASATR SRPLAAELSS HSHQENTKDS GTTEGVWQLV PPSLCKGSHV SQGNEAEQRK EPWDHNENTE EEPVSGSSGS WDQSSQPVFE NENVKCFDRC TGHLAEHTQC GKPQESTGSG YAFSKAVQGR GDTSRQCLPI PADTKGLQDT GGTVHYYWGI PFCPAGVDPN QYTNVILCQL EVYQKSLKMA QRQLVKKRGF GEPVLPRPPF LIQNECGQED QTSDKNEGIS EDMGDEAKEE RQESRASVWH SETKDFQKSP IKSLKQKLLL EEEPTTSRGQ SSQGLFVEET SEEGLKSSEG DNSVPTTQSI AALTSKRSLV LMPESSAEEI TVCPETQLSF LEPLDLNRED SPDSRELPIE VRMAVGDKQV ANREDCMKEN PPPAVSSSTR VSCPLCNQDF PPTKIEQHAM YCNGLMEQET VLTRRRREAK NKSDGRTAAQ PALDANRKEK CYLCKSLVPL GEYQCHVEAC LQLAKVDRED GIEGTRRPRV CAPVEGKQQQ RLKKSKDKGH SQGRLLSLLE QSEHRTTGVE KKPKYSEVRT FRMPSPEVEE ASCSREMQST LSQLNLNESP IKSFVPVSEA TNCLVDFKEQ FAFRSRTKSG RGRRRKS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Ubiquitin-binding protein. Specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
Gene References into Functions
  1. Data indicate that RAP80-deficiency promotes genomic instability and causes an increase in cancer risk consistent with the concept that RAP80 exhibits a tumor suppressor function. PMID: 22896338
  2. a model in which SUMO and Ub modification is coordinated to recruit Rap80 and BRCA1 to DNA damage sites. PMID: 22689573
  3. Mechanistically, loss of RAP80 suppresses recruitment of the BRCA1-A complex to DNA damage sites and abrogates the DNA damage repair process at DNA damage sites. PMID: 22539352
  4. Both ubiquitin-interacting motif 1(UIM1) and UIM2 of RAP80 recognize an Ile 44-centered hydrophobic patch on ubiquitin but neither UIM interacts with the Lys 63-linked isopeptide bond. [RAP80] PMID: 19536136
Subcellular Location
Nucleus.
Protein Families
RAP80 family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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