Recombinant Mouse BTB/POZ domain-containing protein 1 (Btbd1)

Code CSB-YP002840MO
MSDS
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Source Yeast
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Code CSB-EP002840MO
MSDS
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Source E.coli
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Code CSB-EP002840MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002840MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP002840MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Btbd1
Uniprot No.
Alternative Names
Btbd1; GsrpBTB/POZ domain-containing protein 1; Glucose signal-repressing protein
Species
Mus musculus (Mouse)
Expression Region
1-488
Target Protein Sequence
MASLGSAAAG EPATGAEAEP GPPAPPPPPP PPPAPSPSAL GPLLPLQREP LYNWQATKAS LKERFAFLFN SELLSDVRFV LGKGRGAAAA GGPQRIPAHR FVLAAGSAVF DAMFNGGMAT TSAEIELPDV EPAAFLALLR FLYSDEVQIG PETVMTTLYT AKKYAVPALE AHCVEFLTKH LRADNAFMLL TQARLFDEPQ LASLCLDTID KSTVDAISAE GFTDIDIDTL CAVLERDTLS IRESRLFGAI VRWAEAECQR QQLAVTFGNK QKVLGKALSL IRFPLMTIEE FAAGPAQSGI LSDREVVNLF LHFTVNPKPR VEYIDRPRCC LRGKECCINR FQQVESRWGY SGTSDRIRFT VNRRISVVGF GLYGSIHGPT DYQVNIQIIE YEKKQTLGQN DTGFSCDGTA NTFRVMFKEP IEILPNVCYT ACATLKGPDS HYGTKGLKKV VHETPAASKT VFLFFSSPGN NNGTSIEDGQ IPEIIFYT
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to regulate expression levels and/or subnuclear distribution of TOP1, via an unknown mechanism. May play a role in mesenchymal differentiation where it promotes myogenic differentiation and suppresses adipogenesis.
Gene References into Functions
  1. BTBD1 is essential for myogenic differentiation PMID: 15486563
  2. BTBD1 is a key protein of balance between adipogenesis and myogenesis. PMID: 17462629
Subcellular Location
Cytoplasm.
Tissue Specificity
Strongly expressed in heart and skeletal muscle. Weakly expressed in myoblast C2C12 cells, but strongly up-regulated upon their differentiation into myotubes.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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