Recombinant Mouse BarH-like 1 homeobox protein (Barhl1)

Code CSB-YP002558MO
MSDS
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Source Yeast
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Code CSB-EP002558MO
MSDS
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Source E.coli
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Code CSB-EP002558MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002558MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP002558MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Barhl1
Uniprot No.
Alternative Names
Barhl1; Bhx1; Mbh2BarH-like 1 homeobox protein; Bar-class homeodomain protein MBH2; BarH-related homeobox protein 1
Species
Mus musculus (Mouse)
Expression Region
1-327
Target Protein Sequence
MEGSNGFGID SILSHRAGSP ALPKGDPLLG DCRSPLELSP RSESSSDCSS PASPGRDCLE TSTSRPGAAS GPGLDSHLQP GQLSAPAQSR TVTSSFLIRD ILADCKPLAA CAPYSSSGQP AAPEPGGRLA AKAGEDFRDK LDKSVSSASS DSEYKVKEEG DREISSSRDS PPVRLKKPRK ARTAFTDHQL AQLERSFERQ KYLSVQDRME LAASLNLTDT QVKTWYQNRR TKWKRQTAVG LELLAEAGNY SALQRMFPSP YFYPQSLVSN LDPGAALYLY RGPSAPPPAL QRPLVPRILI HGLQGASEPP PPLPPLPGVL PRAAQPR
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Gene References into Functions
  1. Given the important role of Barhl1 in brain development, it was proposed that perturbations of thyroid hormone -mediated transcriptional control of Barhl1 may play a role in the impaired neurodevelopment induced by hypothyroidism. PMID: 22027146
  2. expression of Barhl1 decelerates tumor growth both in human and in murine medulloblastomas. PMID: 21602885
  3. These results suggest that Barhl1 plays an essential role in the migration and survival of cerebellar granule cells and precerebellar neurons and functionally link Barhl1 to the NT-3 signaling pathway during cerebellar development PMID: 15044550
  4. Barhl1 on the other hand was found to act as a transcriptional repressor with repressive activity not restricted to a particular domain of Barhl1. PMID: 16226339
  5. Result suggest that Barhl1 is exclusively transcribed in restricted CNS domains at differential transcription levels which suggest a highly regulated transcriptional mechanism. PMID: 16384683
  6. Barhl1 is crucially required for the survival but not for the specification of zonal layer neurons in the superior colliculus PMID: 16752387
  7. High-level, cell-specific Bargl1expression in the inner ear & CNS depends on both its 5' promoter & 3' enhancer sequences. 2 homeoprotein binding motifs can be occupied & activated by Barhl1. PMID: 18212062
  8. Math1 directly regulates the Mbh genes in CGCs. Furthermore, a dominant-negative form of the Mbh proteins disrupted proper PMID: 18723012

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Subcellular Location
Nucleus.
Protein Families
BAR homeobox family
Database Links
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