Product Details

Purity

>85% (SDS-PAGE)

Target Names

Bloc1s1

Uniprot No.

O55102

Alternative Names

Bloc1s1; Gcn5l1Biogenesis of lysosome-related organelles complex 1 subunit 1; BLOC-1 subunit 1; GCN5-like protein 1

Species

Mus musculus (Mouse)

Expression Region

1-125

Target Protein Sequence

MLSRLLKEHQ AKQNERKELQ EKRRREAIAA ATCLTEALVD HLNVGVAQAY MNQRKLDHEV KTLQVQAAQF AKQTGQWIGM VENFNQALKE IGDVENWARS IELDMRTIAT ALEYVYKGQL QSAPS

Protein Length

Full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. The BORC complex is most probably associated with the cytosolic face of lysosomes, may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor.; May negatively regulate aerobic respiration through mitochondrial protein lysine-acetylation. May counteract the action of the deacetylase SIRT3 by acetylating and regulating proteins of the mitochondrial respiratory chain including ATP5F1A and NDUFA9.

Gene References into Functions

Data show that the mitochondrial enriched GCN5-like 1 protein (GCN5L1) controls hepatic glucose production by regulating FoxO1 protein levels. PMID: 28900165
Acetylation of cardiac mitochondrial fatty acid oxidation enzymes by GCN5L1 significantly upregulates their activity in diet-induced obese mice. PMID: 28526709
TDH, responsible for mitochondrial production of acetyl-CoA in mouse embryonic stem cells, and the acetyltransferase GCN5L1 cooperate to acetylate Lys501 in KBP, allowing its recognition by and degradation via Fbxo15, an F-box protein transcriptionally controlled by the pluripotency core factors and repressed following differentiation. PMID: 28319092
BLOS1 interacts with sorting nexin 2 and the ESCRT-I component TSG101 to mediate the sorting of EGFR into endosomal compartments PMID: 25183008
Here we show that genetic deletion of GCN5L1 has a direct positive effect on the expression and activity of Transcriptional Factor EB (TFEB), which acts as a master regulator of autophagy. PMID: 24356961
Mutations in the human genes encoding Snapin and the BLOS proteins could underlie novel forms of Hermansky-Pudlak syndrome as seen in mouse models PMID: 15102850
Defects in all the 5 known components of BLOC-1, including RP, cause severe Hermansky Pudlak syndrome in mice, suggesting that the subunits are nonredundant and that BLOC-1 plays a key role in organelle biogenesis. PMID: 15265785
These results indicate that the BLOC-1 and AP-3 protein complexes affect the targeting of SNARE and non-SNARE AP-3 cargoes and suggest a function of the BLOC-1 complex in membrane protein sorting. PMID: 16760431

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Subcellular Location

Mitochondrion intermembrane space. Mitochondrion matrix. Cytoplasm, cytosol. Lysosome membrane.

Protein Families

BLOC1S1 family

Database Links

KEGG: mmu:14533

STRING: 10090.ENSMUSP00000026405

UniGene: Mm.30118

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Recombinant Mouse Biogenesis of lysosome-related organelles complex 1 subunit 1 (Bloc1s1)

Product Details

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