Recombinant Mouse Biotinidase (Btd)

Code CSB-YP002854MO
MSDS
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Source Yeast
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Code CSB-EP002854MO
MSDS
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Source E.coli
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Code CSB-EP002854MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002854MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP002854MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Btd
Uniprot No.
Alternative Names
BtdBiotinidase; Biotinase; EC 3.5.1.12
Species
Mus musculus (Mouse)
Expression Region
22-520
Target Protein Sequence
VSSASQEHR EAEYYVAAVY EHPSVLSPNP LELVSRQEAL ELMKQNLDVY EQQVMAAAQK GVQIIVFPED GIHGFNFTRT SIYPFLDFMP SPKLVRWNPC LEPFRFNDTE VLQRLSCMAI KGGMFLVANL GTKQPCLSSD PGCPQDGRYQ FNTNVVFSDN GTLVDRYRKH NLYFEAAFDT PANVDLITFD TPFAGKFGVF TCFDILFFDP AVRLLRDFEV KHIVYPTAWM NQLPLLAAIE IQKAFATAFG VNVLAANIHH PTLGMTGSGI HTPLKSFWYH DMDDPKGHLI IAQVATNPQG LTGTGNTTSE MDPSHRKFLK ILSGDPYCEK DAQEVHCDEA AKWNVNVPPT FHSEMMYDNF TLVPVWGTEG HLQVCSNSLC CHLLYERPTL SKELYALGVF DGLHTVHGTY YIQTCALVKC GGLGFDTCGQ EITEAEGLFD FHLWGNFSTL YIFPLFLTSG MTLDTPDQLG WENDHYFLRK RGLSSGLVTA ALYGRLYERK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalytic release of biotin from biocytin, the product of biotin-dependent carboxylases degradation.
Gene References into Functions
  1. Biotinidase knockout mice show cellular energy deficit and altered carbon metabolism gene expression similar to that of nutritional biotin deprivation. PMID: 24075304
  2. This study demonistrated that biotinidase deficiency mice showed demylination and axonal degeneration. PMID: 22579707
  3. results indicate low expression of biotinidase throughout the brain, but increased concentrations within the dorsal cochlear nucleus, ventral cochlear nucleus, and superior olivary complex as well as in the hair cells and spiral ganglion of the cochlea PMID: 12372635
Subcellular Location
Secreted, extracellular space.
Protein Families
Carbon-nitrogen hydrolase superfamily, BTD/VNN family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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