Recombinant Mouse Calpain-5 (Capn5)

Code CSB-YP004498MO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP004498MO
MSDS
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Source E.coli
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Code CSB-EP004498MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP004498MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP004498MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Capn5
Uniprot No.
Alternative Names
Capn5; Ncl3Calpain-5; EC 3.4.22.-; New calpain 3; nCL-3
Species
Mus musculus (Mouse)
Expression Region
1-640
Target Protein Sequence
MFSCAKAYED QNYSALKRAC LRKKVLFEDP LFPATDDSLY YKGTPGPTVR WKRPKDICDD PRLFVDGISS HDLHQGQVGN CWFVAACSSL ASRESLWQKV IPDWKEQEWN PEKPDSYAGI FHFNFWRFGE WVDVIVDDRL PTVNNQLIYC HSNSKNEFWC ALVEKAYAKL AGCYQALDGG NTADALVDFT GGVSEPIDLT EGDLATDEAK RNQLFERVLK VHSRGGLISA SIKAVTAADM EARLACGLVK GHAYAVTDVR KVRLGHGLLA FFKSEKLDMI RLRNPWGERE WTGPWSDTSE EWQKVSKSER EKMGVTVQDD GEFWMTFEDM CRYFTDIIKC RLINTSYLSI HKTWEEARLH GAWTRHEDPQ QNRSGGCINH KDTFFQNPQY VFEVKKPEDE VLISIQQRPK RSTRREGKGE NLAIGFDIYK VEENRQYRMH SLQHKAASSI YINSRSVFLR TELPEGRYVI IPTTFEPGHT GEFLLRVFTD VPSNCRELRL DEPPRTCWSS LCGYPQQVAQ VHVLGAAGLK DSPTGANSYV IIKCEGEKVR SAVQRGTSTP EYNVKGIFYR KKLAQPITVQ VWNHRVLKDE FLGQVHLKTA PDDLQDLHTL HLQDRSSRQP SDLPGIVAVR VLCSASLTAV
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Calcium-regulated non-lysosomal thiol-protease.
Gene References into Functions
  1. mRNA in situ hybridization experiments were performed to identify the expression of CAPN5 in the mouse eye and brain PMID: 29157313
  2. CAPN5 mutation in hereditary uveitis: the R243L mutation increases calpain catalytic activity and triggers intraocular inflammation PMID: 25994508
  3. CAPN5 is found in punctate domains associated with promyelocytic leukemia (PML) protein within the nucleus. PMID: 24838245
  4. Despite the numerous expression sites of Capn5, the majority of Capn5-null mice were viable and fertile and appeared healthy PMID: 14749380
Protein Families
Peptidase C2 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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