Product Details

Purity

>85% (SDS-PAGE)

Target Names

Cln5

Uniprot No.

Q3UMW8

Alternative Names

Cln5Ceroid-lipofuscinosis neuronal protein 5 homolog; Protein CLN5) [Cleaved into: Ceroid-lipofuscinosis neuronal protein 5 homolog; secreted form]

Species

Mus musculus (Mouse)

Expression Region

34-341

Target Protein Sequence

QRWPVPY KRFSFRPKTD PYCQAKYTFC PTGSPIPVMK DNDVIEVLRL QAPIWEFKYG DLLGHFKLMH DAVGFRSTLT GKNYTIEWYE LFQLGNCTFP HLRPDKSAPF WCNQGAACFF EGIDDKHWKE NGTLSVVATI SGNTFNKVAE WVKQDNETGI YYETWTVRAG PGQGAQTWFE SYDCSNFVLR TYKKLAEFGT EFKKIETNYT KIFLYSGEPI YLGNETSIFG PKGNKTLALA IKKFYGPFRP YLSTKDFLMN FLKIFDTVII HRQFYLFYNF EYWFLPMKPP FVKITYEETP LPTRHTTFTD L

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Plays a role in influencing the retrograde trafficking of lysosomal sorting receptors SORT1 and IGF2R from the endosomes to the trans-Golgi network by controlling the recruitment of retromer complex to the endosomal membrane. Regulates the localization and activation of RAB7A which is required to recruit the retromer complex to the endosomal membrane.

Gene References into Functions

neurogenesis is increased in Cln5-deficient mice, which model the childhood neurodegenerative disorder caused by loss of Cln5. PMID: 28733362
Rssults suggested that CLN2, CLN3 and CLN5 genes may play an important role in early embryonal neurogenesis. PMID: 25303899
The simultaneous loss of both Cln1 and Cln5 genes might enhance the typical pathological phenotypes of these mice by disrupting or downregulating shared or convergent pathogenic pathways. PMID: 23065637
In Cln5-deficient mice, early and significant microglial activation is documented that is already evident at 3 months of age; it increases gradually with age and differs between neurons and glia, with the highest expression in microglia. PMID: 22182690
Study found that CLN5 interacts with several other NCL proteins namely, CLN1/PPT1, CLN2/TPP1, CLN3, CLN6 and CLN8. PMID: 19941651
Mouse Cln5 is a soluble lysosomal glycoprotein expressed throughout embryonic brain, increasing during development; prominent expression is observed in cerebellar Purkinje cells, cerebral neurons, hippocampal pyramidal cells, and hippocampal interneurons. PMID: 15207259
because the role of the functionally uncharacterized Cln5 protein remains unclear these studies may offer clues to the unraveling of its possible function(s) PMID: 18371231
In mouse cerebral cortex, microinjection of VEGF-A disrupted CLN-5 and OCLN and induced loss of barrier function PMID: 19174516
These data provide unexpected evidence for a distinctive sequence of neuron loss in the thalamocortical system of Cln5-/- mice, diametrically opposed to that seen in other forms of NCL. PMID: 19385065

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Subcellular Location

[Ceroid-lipofuscinosis neuronal protein 5 homolog, secreted form]: Lysosome.; [Ceroid-lipofuscinosis neuronal protein 5 homolog]: Membrane; Single-pass type II membrane protein.

Protein Families

CLN5 family

Tissue Specificity

Heart, kidney, liver, spleen, muscle and rectum (at protein level).

Database Links

KEGG: mmu:211286

STRING: 10090.ENSMUSP00000022721

UniGene: Mm.295289

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Recombinant Mouse Ceroid-lipofuscinosis neuronal protein 5 homolog (Cln5)

Product Details

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Target Background

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