Recombinant Mouse Chorion-specific transcription factor GCMb (Gcm2)

Code CSB-YP009324MO
MSDS
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Source Yeast
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Code CSB-EP009324MO
MSDS
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Source E.coli
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Code CSB-EP009324MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009324MO
MSDS
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Source Baculovirus
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Code CSB-MP009324MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Gcm2
Uniprot No.
Alternative Names
Gcm2; GcmbChorion-specific transcription factor GCMb; mGCMb; GCM motif protein 2; Glial cells missing homolog 2
Species
Mus musculus (Mouse)
Expression Region
1-504
Target Protein Sequence
MPADSTQDED AVLSYGMKLT WDINDPQMPQ EPTHFDHFRE WPDGYVRFIY SSQEKKAQRH LSGWAMRNTN NHNGHILKKS CLGVVVCARA CALKDGSHLQ LRPAICDKAR LKQQKKACPN CHSPLELVPC RGHSGYPVTN FWRLDGNAIF FQAKGVHDHP RPESKSETEG RRSALKRQMA SFYQPQKRRS EEPEARSTQD IRGHLNSTAA LEPTELFDMT ADTSFPIPGQ PSPSFPNSDV HRVTCDLPTF QGDIILPFQK YPNPSIYFPG PPWGYELASS GVTGSSPYST LYKDSSVVPD DPDWIPLNSL QYNVSSYGSY ERTLDFTARY HSWKPTHGKP SLEEKVDCEQ CQAVPTSPYY NLELPCRYLP VPAAGTQALQ TVITTTVAYQ AYQHPALKHS DSMQEVSSLA SCTYASENLP MPIYPPALDP QEGVIQAASP SGRAPLKVPG DCQAPRPTLD FPQEADPSGT DGADVWDVCL SGVGSVMGYL DRTGQPFSFD NEDF
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor that binds specific sequences on gene promoters and activate their transcription. Through the regulation of gene transcription, may play a role in parathyroid gland development.
Gene References into Functions
  1. results suggest that MafB regulates later steps of parathyroid development, that is, separation from the thymus and migration toward the thyroid. MafB also regulates the expression of PTH in cooperation with Gcm2 PMID: 21713993
  2. Gcm2 (glial cells missing 2) are involved in the epigenetic regulation of Hes5 transcription by DNA demethylation independently of DNA replication. PMID: 21765423
  3. role of Shh signalling in patterning the pharyngeal pouches and in restricting the expression of the parathyroid marker Gcm2 in the pharyngeal epithelium PMID: 21349263
  4. GCM domain as a new type of Zn-coordinating, sequence-specific DNA-binding domain. PMID: 12297286
  5. Gcm2 is required for the differentiation and survival of parathyroid precursor cells in the parathyroid/thymus primordia. PMID: 17382312

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Subcellular Location
Nucleus.
Database Links
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