Recombinant Mouse Cystatin-8 (Cst8)

Code CSB-YP006096MO
MSDS
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Source Yeast
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Code CSB-EP006096MO
MSDS
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Source E.coli
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Code CSB-EP006096MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006096MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP006096MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Cst8
Uniprot No.
Alternative Names
Cst8; CresCystatin-8; Cystatin-related epididymal spermatogenic protein; Cystatin-related epididymal-specific protein
Species
Mus musculus (Mouse)
Expression Region
20-142
Target Protein Sequence
V GVDQSKNEVK AQNYFGSINI SNANVKQCVW FAMKEYNKES EDKYVFLVDK ILHAKLQITD RMEYQIDVQI SRSNCKKPLN NTENCIPQKK PELEKKMSCS FLVGALPWNG EFNLLSKECK DV
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Performs a specialized role during sperm development and maturation.
Gene References into Functions
  1. A 14-kDa CRES isoform resides in the outer dense fibers of the sperm tail. PMID: 23269664
  2. data on the antimicrobial activities of CRES protein suggest that it is a novel and innate antimicrobial protein which protecting the male reproductive tract against invading pathogens PMID: 23185254
  3. CRES may be a regulator of PC4 in sperm-egg interaction and fertilization. PMID: 22827436
  4. loss of CRES has a significant impact on the structure and functions of the testis and epididymis. However, the observed pathologies were prevalent in aged but not in younger adult mice, suggesting that the effects of the loss of CRES are cumulative PMID: 21051588
  5. These results suggest that mice lacking CRES may have altered local levels of cAMP/PKA activity, or that the CRES defect does not directly involve cAMP/PKA but other signaling pathways that regulate protein tyrosine phosphorylation and capacitation. PMID: 20811015
  6. CRES is a new cross-class inhibitor that may regulate PC2 of PC2-like proteases and suggests a role for CRES in the regulation of prohormone and proprotein processing. PMID: 12479114
  7. cystatin-related epididymal spermatogenic protein (CRES) is a competitive inhibitor of prohormone convertase 2 and may regulate proprotein processing within the reproductive and neuroendocrine systems PMID: 12586766
  8. Cres promoter contains the DNA elements necessary for germ cell and pituitary gland-specific expression but lacks critical sequences necessary for expression in the epididymis and ovary. PMID: 15713831
  9. studies suggest that Cres mRNA and protein are low at peak gonadotrope secretory activity, possibly as a means to allow proprotein processing events to occur that are integral to gonadotrope function PMID: 16837735
  10. The Cres gene displays a specific age-dependent expression pattern in mouse testis and epididymis on both the mRNA and protein level. PMID: 17486270

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Subcellular Location
Secreted.
Protein Families
Cystatin family
Tissue Specificity
Proximal caput region of the epididymis. Lower expression in the testis. Within the testis it is localized to the elongating spermatids, whereas within the epididymis it is exclusively synthesized by the proximal caput epithelium.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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