Recombinant Mouse Dedicator of cytokinesis protein 5 (Dock5), partial

Code CSB-YP007101MO
MSDS
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Source Yeast
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Code CSB-EP007101MO
MSDS
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Source E.coli
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Code CSB-EP007101MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007101MO
MSDS
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Source Baculovirus
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Code CSB-MP007101MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dock5
Uniprot No.
Alternative Names
Dock5; Lr2; RlcDedicator of cytokinesis protein 5; Lens rupture protein 2; Rupture of lens cataract protein
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Guanine nucleotide exchange factor (GEF) for Rho and Rac. GEF proteins activate small GTPases by exchanging bound GDP for free GTP. Along with DOCK1, mediates CRK/CRKL regulation of epithelial and endothelial cell spreading and migration on type IV collagen.
Gene References into Functions
  1. Dock5 and tensin 3 cooperate for osteoclast activity, to ensure the correct organization of podosomes. PMID: 27505886
  2. Tensin3 is a versatile regulator of DOCK5 signaling and cell adhesion. (Review) PMID: 27748980
  3. together with DOCK2 contributes to chemotaxis, reactive oxygen species production, and extracellular trap formation PMID: 25339677
  4. Dock1 and Dock5 are not the important exchange factors regulating Rac activity during the establishment and maintenance of the glomerular barrier PMID: 24365888
  5. results thus identify DOCK5 as a key signaling adaptor that orchestrates remodeling of the microtubule network essential for mast cell degranulation. PMID: 24913231
  6. The atypical Rac1 exchange factor Dock5 is necessary for osteoclast function both in vitro and in vivo. PMID: 21542010
  7. Generated an mDock5-specific antibody, which detected denatured and nascent forms of mDock5. Determined tissue-specific expression patterns and subcellular localization of mDock5 using this antibody. PMID: 19995334
  8. Cataract causing lr2 gene is found in the CXSD mouse, which is a recombinant inbred strain of BALB/c and STS mice PMID: 15297702
  9. Dock5 is dispensable for normal mouse embryogenesis and contribution of Dock5 toward myofiber development. PMID: 18820033

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Involvement in disease
Defects in Dock5 are the cause of rupture of lens cataract. It affects both eyes and is inherited as an autosomal recessive trait. Homozygotes spontaneously develop opacity of the lens at 35-60 days of age. The initial pathological changes appear at about 35 days of age in the deep layer of the posterior cortex as irregular swelling, condensation, degeneration and fragmentation of the lens fibers, leading to rupture of the lens capsule at the posterior pole at 45-100 days of age. Following rupture, the lens nucleus becomes dislocated behind the lens or occasionally in the anterior chamber.
Subcellular Location
Cytoplasm. Cell membrane.
Protein Families
DOCK family
Tissue Specificity
Highly expressed in lens, where it predominantly localizes to anterior epithelial cells, and is weakly expressed in lens fiber (at protein level). Expressed in brain, eye, lung, spleen and kidney, but not in thymus or peripheral blood leukocytes.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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