Recombinant Mouse Deoxyribonuclease-2-alpha (Dnase2)

Code CSB-YP007053MO
MSDS
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Source Yeast
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Code CSB-EP007053MO
MSDS
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Source E.coli
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Code CSB-EP007053MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007053MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP007053MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dnase2
Uniprot No.
Alternative Names
Dnase2; Dnase2a; Dnl2Deoxyribonuclease-2-alpha; EC 3.1.22.1; Acid DNase; Deoxyribonuclease II alpha; DNase II alpha; Lysosomal DNase II
Species
Mus musculus (Mouse)
Expression Region
20-353
Target Protein Sequence
L SCYGDSGQPV DWFVVYKLPA HSGSRDTPKG LTYKYMDQNS DGWQDGVGYI NSSEGAVGRS LQPLYRKNSS QLAFLLYNDQ PPKSSSARDS TGHGHTKGVL LLDQEGGFWL VHSVPRFPPP ASSGAYTWPP NAQTFGQTLL CVSLPFTQFA RIGKQLTYTY PLVYDHKLEG FFAQKLPDLE TVIKNQHVLH EPWNSSVILT SQAGATFQSF AKFGKFGDDL YSGWLAEALG TNLQVQFWQN SPGILPSNCS GAYQVLDVTQ TGFPGPSRLT FSATEDHSKW CVAPQGPWAC VGDMNRNKAE THRGGGTVCT QLPSFWKAFQ SLVKDWKPCI EGS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.
Gene References into Functions
  1. holocrine secretion is a DNase2-mediated form of programmed cell death and suggest that autophagy-dependent metabolism, DNA degradation PMID: 27771328
  2. Data show that Klf1 is expressed in central macrophagse of erythroblastic islands and that it binds and activates the promoter of Dnase2a. PMID: 21807894
Involvement in disease
Absence of Dnase2 is a cause of severe fetal anemia and of perinatal lethality due to malformation of the diaphragm.
Subcellular Location
Lysosome.
Protein Families
DNase II family
Tissue Specificity
Highly expressed in fetal liver macrophages.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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