Recombinant Mouse DnaJ homolog subfamily B member 6 (Dnajb6)

Code CSB-YP007012MO
MSDS
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Source Yeast
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Code CSB-EP007012MO
MSDS
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Source E.coli
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Code CSB-EP007012MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007012MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP007012MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dnajb6
Uniprot No.
Alternative Names
Dnajb6; Hsj2; MrjDnaJ homolog subfamily B member 6; Heat shock protein J2; HSJ-2; MRJ; mDj4
Species
Mus musculus (Mouse)
Expression Region
2-365
Target Protein Sequence
VDYYEVLGV QRHASPEDIK KAYRKQALKW HPDKNPENKE EAERKFKQVA EAYEVLSDAK KRDIYDKYGK EGLNGGGGGG GIHFDSPFEF GFTFRNPDDV FREFFGGRDP FSFDFFEDPF DDFFGNRRGP RGNRSRGAGS FFSTFSGFPS FGSGFPAFDT GFTPFGSLGH GGLTSFSSTS FGGSGMGNFK SISTSTKIVN GKKITTKRIV ENGQERVEVE EDGQLKSLTI NGVADENALA EECQRRGQPT PALAPGPAPA PVRVPSQARP LAPTPAPTPA PTPAPAPAQT PAPSVSTRPQ KPPRPAPTAK LGSKSNWEDD EQDRQRVPGN WDAPMTSAGL KEGGKRKKQK QKEDLKKKKS TKGNH
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity.
Gene References into Functions
  1. Mrj(-/-) chorions at embryonic day 8.5 have expanded Rhox4b expression domains and do not form normal layers of gene expression suggesting that chorion patterning requires Mrj. PMID: 21972064
  2. Data show that Mrj(-/-) embryos exhibit neural tube defects independent of the placenta phenotype, including exencephaly and thin-walled neural tubes, and suggest that Mrj plays an important role in neural stem cell self-renewal. PMID: 19777589
  3. NFATc3 is negatively regulated by class II histone deacetylases through the DnaJ (heat shock protein-40) superfamily member Mrj PMID: 16260608
  4. Study demonstrates here that, in the developing placenta of the mouse, the absence of the Mrj co-chaperone prevents proteasome degradation of keratin 18 intermediate filaments, resulting in the formation of keratin inclusion bodies. PMID: 17409114
Subcellular Location
Cytoplasm, perinuclear region. Nucleus. Cytoplasm, myofibril, sarcomere, Z line.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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