Recombinant Mouse Dystrobrevin alpha (Dtna)

Code CSB-YP863501MO
MSDS
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Source Yeast
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Code CSB-EP863501MO
MSDS
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Source E.coli
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Code CSB-EP863501MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863501MO
MSDS
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Source Baculovirus
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Code CSB-MP863501MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dtna
Uniprot No.
Alternative Names
Dtna; DtnDystrobrevin alpha; DTN-A; Alpha-dystrobrevin
Species
Mus musculus (Mouse)
Expression Region
1-746
Target Protein Sequence
MIEDSGKRGN TMAERRQLFA EMRAQDLDRI RLSTYRTACK LRFVQKKCNL HLVDIWNVIE ALRENALNNL DPNIELNVAR LEAVLSTIFY QLNKRMPTTH QIHVEQSISL LLNFLLAAFD PEGHGKISVF AVKMALATLC GGKIMDKLRY IFSMISDSSG VMVYGRYDQF LREVLKLPTA VFEGPSFGYT EQSARSCFSQ QKKVTLNGFL DTLMSDPPPQ CLVWLPLLHR LANVENVFHP VECSYCHSES MMGFRYRCQQ CHNYQLCQDC FWRGHAGGSH SNQHQMKEYT SWKSPAKKLT NALSKSLSCA SSREPLHPMF PDQPEKPLNL AHIVPPRPVT SMNDTLFSHS VPSSGSPFIT RSSPPKDSEV EQNKMLARAA PAFLKGRGIQ YSLNVADRLA DEHVLIGLYV NMLRNDPPCM LESSNRLDEE HRLIARYAAR LAAESSSSQP TQQRSAPDIS FTIDANKQQR QLIAELENKN REILQEIQRL RVEHEQASQP TPEKAQQNPT LLAELRLLRQ RKDELEQRMS ALQESRRELM VQLEGLMKLL KEEELKQGTQ GASSPRSSPS HTISRPIPMP IRSASACPTP THTPQDSLTG VGGDVQEAFA QSSRRNLRSD LLVAADSITN TMSSLVKELN SEVASETEST VDSEFSRPQF EDLAPSPTSE KAFLAQIHSR KPGYIHGGAA STTHGDMVPE DGDPYTQPED GNYENESVRQ LENELQLEEY LKQKLQDEAY QVSLQG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in synapse maturation and required for normal muscle function.
Gene References into Functions
  1. A phosphorylation site on alpha-dystrobrevin-1 is essential for functional interactions with several proteins at the neuromuscular junction. PMID: 26769899
  2. Loss of alpha dystrobrevin is associated with perturbation in cell cycle progression and nuclei shape. PMID: 25636738
  3. The ErbB2 signaling maintains high efficacy of synaptic transmission by stabilizing the postsynaptic apparatus via phosphorylation of alpha-dystrobrevin1. PMID: 22184199
  4. This is the first demonstration in which assembly of an astroglial protein scaffold containing syntrophin and dystrophin in perivascular astrocytes is dependent on the presence of alpha-dystrobrevin. PMID: 20508543
  5. Fundamental functional differences between the alpha-dystrobrevins of mice and humans raises questions about the use of the mouse as a model animal for Duchenne muscular dystrophy. PMID: 19961569
  6. identified a new dystrobrevin-associated protein, DAMAGE; interacts with the N-terminal region of alpha-dystrobrevin PMID: 14623885
  7. kinetics of the dystrobrevin-Kif5A interaction showed a lower affinity of alpha compared to that of beta-dystrobrevin for binding to kinesin PMID: 16288919
  8. Complete deletion of Dtna isoforms does not reveal any new neuromuscular junction phenotype. PMID: 17933218
  9. Expression of palmitoylated alpha-dystrobrevin prevented the muscular dystrophy observed in the alpha-dystrobrevin-null mice, demonstrating that the altered form of alpha-dystrobrevin was functional. PMID: 18057022
  10. We propose that alpha-DB plays a role in a structure or regulation mechanism unique to MCH-expressing neurons. PMID: 18314094
  11. Mice deficient for alpha-dystrobrevin (adbn(-/-)) exhibit extensive myofiber degeneration and neuromuscular junction abnormalities PMID: 18596960
  12. This study suggests that alpha dystrobrevin is a significant component of the muscle intrinsic program that mediates the formation of complex AChR aggregates. PMID: 19224566

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Subcellular Location
Cytoplasm. Cell junction, synapse. Cell membrane. Note=In peripheral nerves, colocalizes with MAGEE1 in the Schwann cell membrane.
Protein Families
Dystrophin family, Dystrobrevin subfamily
Tissue Specificity
Expressed in skeletal muscle, heart, lung and brain. Sarcolemma and neuromuscular junction in skeletal muscle. Isoform 2 is restricted to the neuromuscular junction. Isoforms 5 and 6 are only expressed in muscle.
Database Links

KEGG: mmu:13527

UniGene: Mm.484047

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