Recombinant Mouse E3 ubiquitin-protein ligase RNF125 (Rnf125)

Code CSB-YP884037MO
MSDS
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Source Yeast
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Code CSB-EP884037MO
MSDS
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Source E.coli
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Code CSB-EP884037MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP884037MO
MSDS
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Source Baculovirus
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Code CSB-MP884037MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Rnf125
Uniprot No.
Alternative Names
Rnf125; E3 ubiquitin-protein ligase RNF125; EC 2.3.2.27; RING finger protein 125
Species
Mus musculus (Mouse)
Expression Region
2-233
Target Protein Sequence
GSLLSSDSS KSAPASATPR TLERSGDSEL PITSFDCSVC LEVLHQPVRT RCGHVFCRSC IATSIKNNNK WTCPYCRAYL PSEGVPATDI AKRMKSEYQN CAECGTLVCL SDMRAHIRTC EKYIDKYGPL LELGDTTARC VCPFCQRELD EDCLLDHCII HHRSERRPVF CPLCHSRPDE SPSTFNGSLI RHLQVSHTLF YDDFIDFDII EEAIIRRVLD RSLLEYVNQS NTT
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins, such as DDX58/RIG-I, MAVS/IPS1, IFIH1/MDA5, JAK1 and p53/TP53. Acts as a negative regulator of type I interferon production by mediating ubiquitination of DDX58/RIG-I at 'Lys-181', leading to DDX58/RIG-I degradation. Mediates ubiquitination and subsequent degradation of p53/TP53. Mediates ubiquitination and subsequent degradation of JAK1. Acts as a positive regulator of T-cell activation.
Gene References into Functions
  1. study identifies new gene-type zinc finger protein 125 (RNF125) as a negative regulator of TRIM14 in the innate antiviral immune response PMID: 28476934
  2. Data indicate that brain from Japanese encephalitis virus (JEV)-infection showed a reciprocal correlation between microRNA miR-15b levels and the expression of ring finger protein 125 (RNF125). PMID: 26202983
  3. RNF125 is enhanced by IFN, these functions constitute a negative regulatory loop circuit for IFN production PMID: 17460044
Subcellular Location
Golgi apparatus membrane; Lipid-anchor.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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