Recombinant Mouse Ensconsin (Map7)

Code CSB-YP013443MO
MSDS
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Source Yeast
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Code CSB-EP013443MO
MSDS
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Source E.coli
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Code CSB-EP013443MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013443MO
MSDS
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Source Baculovirus
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Code CSB-MP013443MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Map7
Uniprot No.
Alternative Names
Map7; Mtap7Ensconsin; Epithelial microtubule-associated protein of 115 kDa; E-MAP-115; Microtubule-associated protein 7; MAP-7
Species
Mus musculus (Mouse)
Expression Region
2-730
Target Protein Sequence
AEQGAGGDG HRGGDGATHS DPASDGYKVQ EKRTAPSRPT STVSGQTSNH SGNKPDPPPV LRVDDRQRLA RERREEREKQ LAARETVWLE REERARQHYE RHLEARKKKL EDQRLKEERR RAAVEEKRRQ RLEEDKERHE AVVRRTMERS QKPRQKSNRW SWGSPLHGSS SIHSGDPDRR SVSTMNLSKH VDPVISKRLS SSSATLLNSP DRARRLQLSP WESSVVSRLL TPTHSFLARS KSTAALSGDT ASCSPIIMPF KAAHSRNPVD RPKLFVTPPE GSARRRTIHG LASHKRERER EHVPFHVSPG ARRTLSPSNL KARSPAPARL WLPSKSMPHL PGTPRPASSL PPGSVRAASA QAPSSSPGNI RPFKREVKVE PEKKDPLPAV KSRVPLVKVE EVTVEEGTPV KPPEPAAPAS APIATPAPAP ATDPAPVPAP SSTVTVGVVP KTSAGTTDPE EATRLLAEKR RLAREQREKE ERERKEKEEL ERQKIEELAR RVAEERSRRE EEARRLEEEQ AREKEELALR LAEEERERWE REEVERVQKQ KEEEARAREE AERARQEREK HFQKEEQERL ERKKRLEEIM RRTRRTETAD KKTTEQRNGD IAKGVLTGEP EVPALPCMAS SGNGESAESP HGVALQQSEV TTESSPDLEK QPNENGMSIQ NENFEEVINL PVGSKASRLD VTNENPEIPL KPILAFNDEG TLGPLPQVDG VQTQQTAEVI
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Microtubule-stabilizing protein that may play an important role during reorganization of microtubules during polarization and differentiation of epithelial cells. Associates with microtubules in a dynamic manner. May play a role in the formation of intercellular contacts. Colocalization with TRPV4 results in the redistribution of TRPV4 toward the membrane and may link cytoskeletal microfilaments.
Gene References into Functions
  1. MAP7 is the TRPV4 C-terminal binding protein; alignment of a.a.798-809 of TRPV4 was important in this interaction; TRPV4 and MAP7 colocalized in lung and kidney; coexpression resulted in redistribution of TRPV4 and enhanced membrane expression of TRPV4 PMID: 14517216
  2. Complementation testing showed that one of these, Mtap7, is disrupted in mshi mice. PMID: 19329343
Subcellular Location
Cytoplasm, perinuclear region. Basolateral cell membrane. Cytoplasm, cytoskeleton. Note=Colocalized on microtubules. An intracellular redistribution is triggered during induction of keratinocyte terminal differentiation from microtubules with a perinuclear localization to cortical microtubules organized in spike-like bundles facing intercellular contacts.
Protein Families
MAP7 family
Tissue Specificity
Highly expressed in the epithelial cells of the kidney tubules and in the absorptive cells of the intestine, and widely distributed in the testis. Expression correlates with the differentiation of certain epithelial cell types: in the adult intestine, mor
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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