Recombinant Mouse Geminin coiled-coil domain-containing protein 1 (Gmnc)

Code CSB-YP662387MO
MSDS
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Source Yeast
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Code CSB-EP662387MO
MSDS
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Source E.coli
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Code CSB-EP662387MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP662387MO
MSDS
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Source Baculovirus
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Code CSB-MP662387MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Gmnc
Uniprot No.
Alternative Names
Gmnc; Gemc1; Gm606Geminin coiled-coil domain-containing protein 1
Species
Mus musculus (Mouse)
Expression Region
1-333
Target Protein Sequence
MNSILPCQDQ YFVGGQSYNC PYSTTTSESG VDVSKETWVS FWAAGLLDNT ELQQAPQVLE SPSELSFPVL DSCSWEEAQL SSQLYRNKQL QDTLLQKEEE LARLHEENNH LRQYLNSTLV KRLEEKAKKL LSSDEFSKVF GKLRKEKRKP KEHRHSPAEI PQFKTAKRNL STEFSNCEEQ PGPHVDPWVL QTLGLKDLNT IDDTLPANYS ATTSHPRAVP SISSLFVHDA NGYANVPRDL HLDYVGQGTH GSHTTSIHQE DCHSFPRLSN PPVRVQTLPY HTADVSPNKT EMAFSTSLSP HCNVKTHSFH QGQAFVRRDE EGGWKFTWVP KQT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulator of DNA replication. Promotes initiation of chromosomal DNA replication by mediating TOPBP1- and CDK2-dependent recruitment of CDC45L onto replication origins.
Gene References into Functions
  1. The authors found that GEMC1-deficient mice are growth impaired, develop hydrocephaly with a high penetrance, and are infertile, due to defects in the formation of multiciliated cells in the brain, respiratory tract, and germline. PMID: 26933123
  2. GemC1 directly transactivates the promoters of MCIDAS and FOXJ1. PMID: 26882546
  3. Results suggest that Mcidas and GemC1 are key players in the generation of multiciliated ependymal cells of the adult neurogenic niche. PMID: 26395491
Subcellular Location
Nucleus.
Protein Families
GEMC1 family
Database Links
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