Product Details

Purity

>85% (SDS-PAGE)

Target Names

Hap1

Uniprot No.

O35668

Alternative Names

Hap1; Huntingtin-associated protein 1; HAP-1

Species

Mus musculus (Mouse)

Expression Region

1-628

Target Protein Sequence

MRPKEQVQSG AGDGTGSGDP AAGTPTTQPA VGPAPEPSAE PKPAPAQGTG SGQKSGSRTK TGSFCRSMII GDSDAPWTRY VFQGPYGPRA TGLGTGKAEG IWKTPAAYIG RRPGVSGPER AAFIRELQEA LCPNPPPTKK ITEDDVKVML YLLEEKERDL NTAARIGQSL VKQNSVLMEE NNKLETMLGS AREEILHLRK QVNLRDDLLQ LYSDSDDDDD EEDEEDEEEG EEEEREGQRD QDQQHDHPYG APKPHPKAET AHRCPQLETL QQKLRLLEEE NDHLREEASH LDNLEDEEQM LILECVEQFS EASQQMAELS EVLVLRLEGY ERQQKEITQL QAEITKLQQR CQSYGAQTEK LQQMLASEKG IHSESLRAGS YMQDYGSRPR DRQEDGKSHR QRSSMPAGSV THYGYSVPLD ALPSFPETLA EELRTSLRKF ITDPAYFMER RDTHCREGRK KEQRAMPPPP AQDLKPPEDF EAPEELVPEE ELGAIEEVGT AEDGQAEENE QASEETEAWE EVEPEVDETT RMNVVVSALE ASGLGPSHLD MKYVLQQLSN WQDAHSKRQQ KQKVVPKDSP TPQQQTNMGG GILEQQPRVP TQDSQRLEED RATHSPSARE EEGPSGAT

Protein Length

Full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Originally identified as neuronal protein that specifically associates with HTT/huntingtin and the binding is enhanced by an expanded polyglutamine repeat within HTT possibly affecting HAP1 interaction properties. Both HTT and HAP1 are involved in intracellular trafficking and HAP1 is proposed to link HTT to motor proteins and/or transport cargos. Seems to play a role in vesicular transport within neurons and axons such as from early endosomes to late endocytic compartments and to promote neurite outgrowth. The vesicular transport function via association with microtubule-dependent transporters can be attenuated by association with mutant HTT. Involved in the axonal transport of BDNF and its activity-dependent secretion; the function seems to involve HTT, DCTN1 and a complex with SORT1. Involved in APP trafficking and seems to facilitate APP anterograde transport and membrane insertion thereby possibly reducing processing into amyloid beta. Involved in delivery of gamma-aminobutyric acid (GABA(A)) receptors to synapses; the function is dependent on kinesin motor protein KIF5 and is disrupted by HTT with expanded polyglutamine repeat. Involved in regulation of autophagosome motility by promoting efficient retrograde axonal transport. Seems to be involved in regulation of membrane receptor recycling and degradation, and respective signal transduction, including GABA(A) receptors, tyrosine kinase receptors, EGFR, IP3 receptor and androgen receptor. Among others suggested to be involved in control of feeding behavior (involving hypothalamic GABA(A) receptors), cerebellar and brainstem development (involving AHI1 and NTRK1/TrkA), postnatal neurogenesis (involving hypothalamic NTRK2/TrkB regulating the number of Npyr1-expressing cells), and ITPR1/InsP3R1-mediated Ca(2+) release (involving HTT and possibly the effect of mutant HTT). Via association with DCTN1/dynactin p150-glued and HTT/huntingtin involved in cytoplasmic retention of REST in neurons. May be involved in ciliogenesiss; however, reports are conflicting: PubMed:21985783 reports that Hap1 is required for ciliogenesis in primary cortical neurons and proposes that HTT interacts with PCM1 through HAP1; PubMed:23532844 reports that mice with disrupted Hap1 display normal cilium formation and function. Involved in regulation of exocytosis. Isoform A but not isoform B seems to be involved in formation of cytoplasmic inclusion bodies (STBs). In case of anomalous expression of TBP, can sequester a subset of TBP into STBs; sequestration is enhanced by an expanded polyglutamine repeat within TBP.

Gene References into Functions

This study also validated the interaction between HAP1 and Sec23A using co-immunoprecipitation experiments with endogenous proteins. This manuscript also reports on the high confidence interacting partners of HAP1 identified by the non-biased approach used in this study, which interestingly, consists of predominantly trafficking-related proteins PMID: 28259758
HAP1 co-localizes with synapsin I in cortical neurons as discrete puncta PMID: 27315547
Early loss of Hap1 significantly reduces postnatal hippocampal neurogenesis, and leads to adult depressive-like behavior. PMID: 25875952
Hap1 interacts with Bcr on microtubules to regulate neuronal differentiation. PMID: 25671650
HAP1 regulates exocytosis by influencing the morphological docking of vesicles at the plasma membrane, the ability of vesicles to be released rapidly upon stimulation, and the early stages of fusion pore formation. PMID: 24366265
these studies identify htt and HAP1 as regulators of autophagosome transport in neurons PMID: 24453320
In the absence of HAP1, postnatal hypothalamic neurons exhibited reduced receptor tropomyosin-related kinase B (TRKB) levels. PMID: 24355921
Hap1-Tsc1 interaction regulates neuronal mTORC1 signaling and neuronal morphogenesis. PMID: 24227713
Mutant mice with Hap1 deficiency in pancreatic beta-cells had impaired glucose tolerance and decreased insulin release in response to intraperitoneally injected glucose. PMID: 21544547
Loss of Htt impaired the retrograde trafficking of PCM1 and thereby reduced primary cilia formation. PMID: 21985783
Results indicate that the Hap1-Ahi1 complex in the brainstem works as a sensor for insulin signals in feeding control. PMID: 21146532
Hap1 is critical for the transport of multiple proteins to the nerve terminals to maintain the integrity of neuronal processes PMID: 20304926
results demonstrate that HAP-1 plays an essential role in regulating postnatal feeding PMID: 11971876
HAP-1 is highly expressed in hypothalamus. Mice lacking HAP1 die after birth because of depressed feeding. The effect of mutant huntingtin on HAP1 and EGFR signaling may contribute to hypothalamic neurodegeneration and weight loss in Huntington disease PMID: 12890790
HAP1 facilitates potentiation of InsP3 receptor-1 mediated Ca2+ release. PMID: 15379999
may be involved in protective pathways that contribute to the resistance of interneurons to neurodegeneration in HD. PMID: 15548548
Results provide evidence linking hypothalamic Hap1 to GABA in the stimulation of feeding and suggest that this mechanism is involved in the feeding-inhibitory actions of insulin in the brain. PMID: 16604089
phosphorylation-dependent interaction of HAP1A with 14-3-3 regulates HAP1 function by influencing its association with kinesin light chain and trafficking in neuronal processes. PMID: 17166838
HAP1 can sequester a subset of TBP protein away from the nucleus; extranuclear TBP sequestration is quantitatively influenced by the TBP polyQ repeat. These results suggest HAP1 could provide protection from SCA17 neuropathology. PMID: 17868456
Hap1 interacts with Ahi1 to regulate cerebellar and brainstem development in mice. PMID: 18636121

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Subcellular Location

Cytoplasm. Cell junction, synapse, presynapse. Cytoplasm, cytoskeleton. Cell projection, dendritic spine. Cell projection, dendrite. Cell projection, axon. Lysosome. Endoplasmic reticulum. Mitochondrion. Nucleus. Cytoplasmic vesicle, autophagosome. Early endosome. Cell projection, growth cone.; [Isoform B]: Cytoplasm.; [Isoform A]: Cell projection, growth cone. Cell projection, neuron projection. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Cell junction, synapse, presynapse.

Database Links

KEGG: mmu:15114

STRING: 10090.ENSMUSP00000133356

UniGene: Mm.281700

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Recombinant Mouse Huntingtin-associated protein 1 (Hap1)

Product Details

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Target Background

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