Recombinant Mouse Insulin-2(Ins2)

Code CSB-YP360644MO
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Source Yeast
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Code CSB-EP360644MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP360644MO
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Source Baculovirus
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Code CSB-MP360644MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Ins2
Uniprot No. P01326
Alternative Names Ins2; Ins-2; Insulin-2 [Cleaved into: Insulin-2 B chain; Insulin-2 A chain]
Species Mus musculus (Mouse)
Expression Region 25-110
Target Protein Sequence FVKQHLCGSHLVEALYLVCGERGFFYTPMSRREVEDPQVAQLELGGGPGAGDLQTLALEVAQQKRGIVDQCCTSICSLYQLENYCN
Protein Length Cytoplasmic domain
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Gene References into Functions
  1. High INS2 expression is associated with weight gain and obesity. PMID: 29122848
  2. Data (including data from studies in knockout mice) suggest that Ins2 is involved in impaired nociception/diabetic neuropathy; here, mice heterozygous for mutant Ins2 exhibit (a) significant loss of intra-epidermal nerve fibers, (b) markedly reduced responsiveness to heat in dorsal root ganglion neurons, and (c) mostly unchanged function of cold-sensitive neurons; such mice become diabetic soon after weaning. PMID: 29875100
  3. These results suggest that PABP interacts with HuD in basal glucose conditions making translation inhibitory complex, however upon glucose stimulation this association is affected and PABP is acted upon by PDI resulting in stimulation of insulin translation. PMID: 29590218
  4. cTAGE5 deletion in pancreatic beta cells impairs proinsulin trafficking and insulin biogenesis in mice. PMID: 29133483
  5. report that EndMTs occur in the diabetic endothelium of Ins2Akita/wt mouse, and show that induction of sex determining region Y-box 2 (Sox2) is a mediator of excess BMP signaling that results in activation of EndMTs and increased vascular calcification PMID: 27936229
  6. Transplantation of transduced hematopoietic stem cells (HSCs) expressing proinsulin II prevents diabetes development. PMID: 26784909
  7. Wnt3a increased the expression of NeuroD1 and Ins2 in the hypothalamus. PMID: 26956881
  8. have characterized the distinctive sex-specific phenotypes exhibited by the ApoE(-/-):Ins2(+/Akita) mouse model and present evidence for the action of sex hormones on pancreatic beta-cell function PMID: 26597883
  9. Data indicate that insulin/incomplete Freund's adjuvant (IFA) does not prevent but induces diabetes in RIP-CD80GP transgenic mice. PMID: 24387268
  10. RORalpha is a transcriptional activator of insulin. PMID: 24583012
  11. Mice deficient in coinhibitory PD-L1 or PD-1 molecules (PD-L1(-/-) and PD-1(-/-) mice), were used to study induction of preproinsulin (ppins)-specific CD8 T-cell responses and experimental autoimmune diabetes. PMID: 23977133
  12. Akita mouse has a mutation in Ins2 and is a model of the effects of maternal and paternal hyperglycemia in wildtype offspring PMID: 23209676
  13. Cortical bone was affected in STZ but not Ins2(+/-) mice. PMID: 22886636
  14. proSAAS as a novel down-regulated target of Pax6 PMID: 23056534
  15. Leptin administration improves dyslipidemia and reduces atherosclerosis in type 1 diabetic Ins2(+/Akita):apoE(-/-) mice. PMID: 23099119
  16. Ins2(Akita) mouse is a good model for later-onset DR, modeling both early and some late disease signs. PMID: 23221078
  17. The Akita(ins2) type 1 diabetic model is protected against systolic failure due to increased NCX1 expression via a CXCR4/NF-kappaB pathway. PMID: 22610174
  18. Integrin alpha1KOAkitaKO Balb/c mouse represents a promising model presenting with most features of human diabetic nephropathy. PMID: 22297672
  19. these results indicate that CaMKIIdelta2 downregulates insulin gene expression by Ser142 phosphorylation of CREB and reducing binding of CREB to CBP. PMID: 22554507
  20. HuD controls insulin II translation PMID: 22387028
  21. Data show that TMED6 mRNA is highly and selectively expressed in pancreas,and knockdown of TMED6 gene expression in Min6 beta cells decreased insulin secretion. PMID: 22129529
  22. The results indicated that intraislet ghrelin does not play a major role in the regulation of insulin secretion in vivo. PMID: 22114024
  23. Data suggest that high glucose mediates the recruitment of p300, CBP, PCAF, and GCN5 to the insulin promoter and that all four histone acetyltransferase (HATs) are important for insulin gene expression. PMID: 21774670
  24. Raf-1 specifically and acutely regulates insulin 2 mRNA via negative action on Foxo1 PMID: 21817126
  25. The findings demonstrate that the perturbation of proinsulin homeostasis results in defects in the subsequent conversion process of proinsulin and is a contributor to the occurrence of disproportionate hyperproinsulinemia in diabetes. PMID: 21723250
  26. Exaggerated hypercholesterolemia and atherosclerosis in spontaneously diabetic Ins2(+/Akita):apoE(-/-) mice may be attributable to impaired lipoprotein clearance in the setting of diminished expression of LSR. PMID: 21447785
  27. The transcriptional programs in both endoneurial and neuronal compartments of the peripheral nerve are relatively resistant to the onset of hyperglycemia and hypoinsulinemia in Ins2 mice. PMID: 20520806
  28. Alternative splicing of insulin mRNA in mice could result in an additional level of regulation in insulin biosynthesis. PMID: 20153322
  29. These studies identify strong effects of genetic background to modify the renal phenotype associated with the Ins2(C96Y) mutation. PMID: 20042456
  30. Type 1 diabetic cardiomyopathy in the Akita (Ins2WT/C96Y) mouse model is characterized by lipotoxicity and diastolic dysfunction with preserved systolic function. PMID: 19801494
  31. The interval between Ins2 and Ascl2 is dispensable for imprinting centre function in the mouse model of Beckwith-Wiedemann Syndrome. PMID: 19684026
  32. mutated in iddm in mice PMID: 11981430
  33. findings suggest that the organelle dysfunction resulting from the intracellular accumulation of misfolded proinsulin 2 is primarily responsible for the defect of coexisting wild-type insulin secretion in Akita beta-cells PMID: 12540615
  34. Transcriptional up-regulation of the remaining functional insulin gene in Ins2-/- mice could potentially contribute to beta-cell adaptation. PMID: 12745665
  35. Wild-type preproinsulin-2-expressing pancreatic islets transplanted in preproinsulin-2-deficient mice elicit a mononuclear cell infiltration and insulin antibodies; graft infiltration is further increased by immunization with preproinsulin-2 peptides. PMID: 14688305
  36. A missense mutation of the insulin 2 gene (Cys96Tyr) disrupts 1 of the 2 interchain disulfide bonds resulting in accumulation of misfolded protein in pre-Golgi intermediates. PMID: 15033933
  37. platelet-derived growth factor-induced cell proliferation is inhibited by insulin PMID: 15525682
  38. Pancreatic beta-cells that glucose stimulates the recruitment of ribosome-associated proinsulin mRNA, located in the cytoplasm, to the ER, the site of proinsulin synthesis; this plays an important role in glucose-stimulated proinsulin synthesis. PMID: 15972000
  39. Proinsulin-2 gene expression by radioresistant thymic epithelial cells is involved in the induction of self-tolerance, and additional factors are required to induce islet abnormalities. PMID: 16785498
  40. Munich Ins2 mutant mice are a valuable model to study the mechanisms of beta-cell dysfunction and death during development of diabetes. PMID: 17303807
  41. diabetes causes fusion between Proins-P bone marrow-derived cells and hepatocytes PMID: 17360472
  42. Cre recombinase is controlled by a short fragment of the rat insulin II gene promoter PMID: 17533574
  43. The data underline a direct role for Aire in tissue-restricted antigens expression and suggest that modulation of Aire has a potential to control central tolerance and autoimmunity. PMID: 17599412
  44. misfolded proinsulin causes enlargement of pre-Golgi intermediates which indicates their involvement in protein quality control. PMID: 17647009
  45. Nonobese, insulin-deficient Ins2(Akita) mice develop type 2 diabetes phenotypes including peripheral and hepatic insulin resistance and cardiac remodeling. PMID: 17911348
  46. Insulin II is a direct target of RHOX5. PMID: 18184911
  47. Arterial superoxide generated from diverse sources may potentiate the contractions of carotid arteries in Ins2(Akita) diabetic mice. PMID: 18788099
  48. Mutant huntingtin disrupts intracellular transport and insulin secretion by interacting with tubulin beta 5. PMID: 19628478
  49. Ins2 expression by NOD bone marrow-derived cells did not delay diabetes development in NOD-Ins2-/- mice. PMID: 19874548

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Subcellular Location Secreted.
Protein Families Insulin family
Database Links

KEGG: mmu:16334

STRING: 10090.ENSMUSP00000000220

UniGene: Mm.4946

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